Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,664,759 G→A 6.6% A208T (GCA→ACA)  acnA‑II → aconitate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,664,7590GA 6.6% 179.7 / 2.1 60A208T (GCA→ACA) acnA‑IIaconitate hydratase
Reads supporting (aligned to +/- strand):  ref base G (31/25);  new base A (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCACCGACCTGG  >  NC_002947/2664613‑2664903
                                                                                                                                                  |                                                                                                                                                
tCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCa                                                                                                                                                >  8:135050/1‑149 (MQ=255)
tCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCa                                                                                                                                                >  2:194179/1‑149 (MQ=255)
    gAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTgg                                                                                                                                            <  6:37590/149‑1 (MQ=255)
    gAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTgg                                                                                                                                            <  1:264010/149‑1 (MQ=255)
    gAACGTCGACGGGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTgg                                                                                                                                            <  7:134427/149‑1 (MQ=255)
     aaCGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTggg                                                                                                                                           <  1:171353/149‑1 (MQ=255)
            aCGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATc                                                                                                                                    <  3:27535/149‑1 (MQ=255)
              gTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGc                                                                                                                                  >  2:112062/1‑149 (MQ=255)
                 aTCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCa                                                                                                                                <  1:194179/148‑1 (MQ=255)
                           gCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAggc                                                                                                                     <  3:160378/149‑1 (MQ=255)
                                        gCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATACACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGcc                                                                                                         >  3:63655/1‑148 (MQ=255)
                                           ccAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGaa                                                                                                      >  2:254691/1‑148 (MQ=255)
                                               aTCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGt                                                                                                 >  2:31952/1‑149 (MQ=255)
                                                   aCCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATg                                                                                             >  1:68989/1‑149 (MQ=255)
                                                    ccTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGc                                                                                            >  4:26755/1‑149 (MQ=255)
                                                    ccTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGc                                                                                            >  3:197016/1‑149 (MQ=255)
                                                        gagaAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTc                                                                                          <  1:112062/147‑1 (MQ=255)
                                                          gaagaTGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGcc                                                                                      <  1:31952/149‑1 (MQ=255)
                                                                gTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTg                                                                                                <  1:41345/133‑1 (MQ=255)
                                                                gTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTg                                                                                                >  2:41345/1‑133 (MQ=255)
                                                                   aCCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGcctcct                                                                             >  1:247811/1‑149 (MQ=255)
                                                                   aCCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGcctcct                                                                             >  3:242924/1‑149 (MQ=255)
                                                                    ccGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGc                                                                                                          <  3:15720/119‑1 (MQ=255)
                                                                    ccGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGc                                                                                                          >  4:15720/1‑119 (MQ=255)
                                                                     cGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTgg                                                                           <  5:209606/149‑1 (MQ=255)
                                                                     cGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATACACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTgg                                                                           <  4:63655/149‑1 (MQ=255)
                                                                            ccACAGCGACCGCGGTGTCGCCTACCCGGACCCCTGCGTCGGCACCAACCGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTc                                                                    >  2:195698/1‑149 (MQ=255)
                                                                               caGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGc                                                                 >  5:212862/1‑149 (MQ=255)
                                                                                  cGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGAACTGCGTGTGATCTCCATCTGCGTAGGCGGCCTGGAAGCCGAAAACGTCATGCTCGCCCGCGCCTCCTGCATCCGTCTGcag                                                               >  8:99729/1‑146 (MQ=255)
                                                                                     ccGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGaaa                                                           >  4:179126/1‑149 (MQ=255)
                                                                                     ccGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGaaa                                                           <  7:135050/149‑1 (MQ=255)
                                                                                          gtgtCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAAtcgtc                                                      >  2:246009/1‑149 (MQ=255)
                                                                                          gtgtCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGCGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAAtcgtc                                                      <  4:197016/149‑1 (MQ=255)
                                                                                             tCGCCTAACCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGc                                                   >  7:21761/1‑149 (MQ=255)
                                                                                               gCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTg                                                                                                               >  4:254799/1‑87 (MQ=255)
                                                                                               gCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTg                                                                                                               <  3:254799/87‑1 (MQ=255)
                                                                                                ccTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATACACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCgg                                                              >  3:36953/1‑135 (MQ=255)
                                                                                                ccTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATACACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCgg                                                              <  4:36953/135‑1 (MQ=255)
                                                                                                   aCCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTg                                                                                                                                             <  3:232410/53‑1 (MQ=255)
                                                                                                   aCCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTg                                                                                                                                             >  4:232410/1‑53 (MQ=255)
                                                                                                   aCCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAg                                             >  8:135786/1‑149 (MQ=255)
                                                                                                    cccGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAg                                             <  1:254691/148‑1 (MQ=255)
                                                                                                     ccGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGaaa                                                                                                     <  8:171085/91‑1 (MQ=255)
                                                                                                     ccGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGaaa                                                                                                     >  7:171085/1‑91 (MQ=255)
                                                                                                     ccGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCt                                           >  1:173608/1‑149 (MQ=255)
                                                                                                     ccGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCt                                           >  8:213143/1‑149 (MQ=255)
                                                                                                     ccGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCt                                           <  8:81203/149‑1 (MQ=255)
                                                                                                       ggACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGa                                         <  7:213143/149‑1 (MQ=255)
                                                                                                       ggACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGa                                         <  1:246009/149‑1 (MQ=255)
                                                                                                          cacCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTg                                      <  5:244030/149‑1 (MQ=255)
                                                                                                             cTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGc                                    >  6:251362/1‑148 (MQ=255)
                                                                                                                            cAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACa                    <  7:135786/149‑1 (MQ=255)
                                                                                                                             aGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACAt                   >  8:51294/1‑149 (MQ=255)
                                                                                                                               ccACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCa                 >  8:227702/1‑149 (MQ=255)
                                                                                                                                     gccgcATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCa           >  1:223375/1‑149 (MQ=255)
                                                                                                                                     gccgcATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCa           <  4:242924/149‑1 (MQ=255)
                                                                                                                                        gcATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCAcc         >  8:174980/1‑148 (MQ=255)
                                                                                                                                          aTGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCACCGAc      <  3:26755/149‑1 (MQ=255)
                                                                                                                                               gATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCACCGACCTgg  >  5:96787/1‑148 (MQ=255)
                                                                                                                                               gATGCACTGGGTGTGATCGCCATCGGCGTAGCCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGACTCCTGGATGCGTCTCCCGGAAATCGTCGGCGTCGAGCTGACTGCCAAGCTGGCCCCGAACATCACAGCCACCGCCCtcg  >  8:251585/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGCACCAGATCAACCTGGAGAAGATGTCACCGGTGGTCCACAGCGACCGCGGTGTCGCCTACCCGGACACCTGCGTCGGCACCGACAGCCACACGCCGCATGTCGATGCACTGGGTGTGATCGCCATCGGCGTAGGCGGCCTGGAAGCCGAAAACGTGATGCTCGGCCGCGCCTCCTGGATGCGTCTGCCGGAAATCGTCGGCGTCGAGCTGACTGGCAAGCTGGCCCCGAACATCACTGCCACCGACCTGG  >  NC_002947/2664613‑2664903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑