Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,747,196 C→T 10.2% Q191Q (CAG→CAA)  PP_2402 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,747,1960CT10.2% 102.4 / 2.8 39Q191Q (CAG→CAA) PP_2402sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (21/14);  new base T (2/2);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

GATCGGGGTTTGCAGGTCGTGGGAAATGGCCGCCAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGCTACCGGGACCGGTGCTG  >  NC_002947/2747049‑2747344
                                                                                                                                                   |                                                                                                                                                    
gATCGGGGTTTGCAGGTCGTGGGAAATGGCCGCCAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCt                                                                                                                                                     >  6:271522/1‑149 (MQ=255)
            cAGGTCGTGGGAAATGGCCGCCAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGACACGGCAGCGTAACGCACCTCGCGTGGTCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTc                                                                                                                                           >  8:58012/1‑147 (MQ=255)
             aGGTCGTGGGAAATGGCCGCCAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAg                                                                                                                                        <  5:2151/149‑1 (MQ=255)
                             ccgccAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTgg                                                                                                                        >  7:187226/1‑149 (MQ=255)
                             ccgccAGCAACTGCATGCGGTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTgg                                                                                                                        <  7:101610/149‑1 (MQ=255)
                              cgccAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTggg                                                                                                                       <  3:72010/149‑1 (MQ=255)
                              cgccAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTggg                                                                                                                       >  7:210578/1‑149 (MQ=255)
                               gccAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGc                                                                                                                      <  6:172679/149‑1 (MQ=255)
                               gccAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGc                                                                                                                      <  5:271522/149‑1 (MQ=255)
                                          cATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGt                                                                                                             <  8:210578/147‑1 (MQ=255)
                                           aTGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAg                                                                                                          >  7:231117/1‑149 (MQ=255)
                                                   cTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGa                                                                                                  >  3:8038/1‑149 (MQ=255)
                                                   cTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGa                                                                                                  >  7:64283/1‑149 (MQ=255)
                                                                 caATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTAcc                                                                                    <  5:60382/149‑1 (MQ=255)
                                                                           cTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCgcca                                                                                >  4:128599/1‑143 (MQ=255)
                                                                           cTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCgcca                                                                                <  3:128599/143‑1 (MQ=255)
                                                                              cAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTa                                                                        >  6:58473/1‑148 (MQ=255)
                                                                                 ggCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTAcaca                                                                    <  7:44216/149‑1 (MQ=255)
                                                                                   cGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACacagc                                                                  <  4:8038/149‑1 (MQ=255)
                                                                                    gTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGTTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCa                                                                                            <  5:234098/122‑1 (MQ=255)
                                                                                    gTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGTTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCa                                                                                            <  1:260704/122‑1 (MQ=255)
                                                                                    gTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGTTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCa                                                                                            >  2:260704/1‑122 (MQ=255)
                                                                                    gTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGTTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCa                                                                                            >  6:234098/1‑122 (MQ=255)
                                                                                             cACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACacag                                                                   <  6:71878/138‑1 (MQ=255)
                                                                                             cACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACacag                                                                   >  5:71878/1‑138 (MQ=255)
                                                                                              aCGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAacagcacagc                                                       >  7:68877/1‑149 (MQ=255)
                                                                                                     cGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTg                                                 >  8:141132/1‑148 (MQ=255)
                                                                                                      gTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGAACACAGCAACAGCAcatccaccaga                                                >  8:226943/1‑143 (MQ=255)
                                                                                                      gTAACGCACCTCGCGTGGGCCGCTTTCATCAAGTTGAACGCCGGGCTGGTCCGGGTCTAGGTTGTCCACTGCCTGGGCCAGACTGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGa                                                <  7:58012/148‑1 (MQ=255)
                                                                                                         aCGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATca                                            <  5:58473/149‑1 (MQ=255)
                                                                                                               cTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCATGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAgcagca                                      >  4:97568/1‑149 (MQ=255)
                                                                                                                tCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGcac                                     >  2:254547/1‑149 (MQ=255)
                                                                                                                             tttCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGcaaccaa                        >  5:110445/1‑149 (MQ=255)
                                                                                                                                 aTCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGCCGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCCCCACGGGCAACCAACggg                    >  3:137294/1‑149 (MQ=255)
                                                                                                                                  tCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGc                   >  3:114957/1‑149 (MQ=255)
                                                                                                                                   ccAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCCGCACCACGGGcacccaccagggc                  >  7:39257/1‑142 (MQ=255)
                                                                                                                                     aGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGCTAc                >  6:213859/1‑149 (MQ=255)
                                                                                                                                                 ggCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGCTACCGGGACCGGTGc    >  3:140887/1‑149 (MQ=255)
                                                                                                                                                  gCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGCTACCGGGACCGGTGCt   <  4:140887/149‑1 (MQ=255)
                                                                                                                                                   cTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGCTACCGGGACCGGTGCTg  <  3:97568/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GATCGGGGTTTGCAGGTCGTGGGAAATGGCCGCCAGCAACTGCATGCGTTCCTTGAGGTGGGCAGCAATGCGCGCCTGCAGGGCGTTGAAGGCCACGGCAGCGTAACGCACCTCGCGTGGGCCGCTTTCATCCAGTTGAACGCCGGGCTGGTCCGGGTCGAGGTTGTCCACTGCCTGGGCCAGACGGGTTAGCGGGCCGATGGCCAGGCGTACCGCCAGCCAGGTACACAGCAACAGCACAGCCAGCTGAATCAGCAGCACCACGGGCAACCAACGGGCTACCGGGACCGGTGCTG  >  NC_002947/2747049‑2747344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑