Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,086 G→C 10.5% G471A (GGG→GCG) ‡ czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,0860GC10.5% 106.0 / 4.0 38G471A (GGG→GCG) ‡czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (19/15);  new base C (2/2);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CCCATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGAT  >  NC_002947/2754939‑2755230
                                                                                                                                                    |                                                                                                                                                 
cccATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCggg                                                                                                                                                  <  5:47940/149‑1 (MQ=255)
                 cACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGCaaagatcg                                                                                                                                 <  1:142005/149‑7 (MQ=25)
                           tGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATg                                                                                                                      >  3:134825/1‑149 (MQ=255)
                            gCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑g                                                                                                                                     <  6:222553/133‑1 (MQ=255)
                            gCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑g                                                                                                                                     >  5:222553/1‑133 (MQ=255)
                              tGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGcc                                                                                                                   >  5:172677/1‑149 (MQ=255)
                              tGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGcc                                                                                                                   >  7:132790/1‑149 (MQ=255)
                                   cgcgGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTAc                                                                                                              >  1:240259/1‑149 (MQ=255)
                                       gcgAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTg                                                                                                          <  6:165688/149‑1 (MQ=255)
                                                     gAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCGTTACCGTGGTCATGGCCCTGtt                                                                                            >  5:241546/1‑149 (MQ=255)
                                                     gAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGACTTTACCGTGGTCATGGCCCTGtt                                                                                            >  8:247208/1‑149 (MQ=255)
                                                      aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GAt                                                                                                                                   >  6:202100/1‑109 (MQ=255)
                                                      aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GAt                                                                                                                                   <  5:202100/109‑1 (MQ=255)
                                                         tgtTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgc                                                                                        <  6:172677/149‑1 (MQ=255)
                                                         tgtTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgc                                                                                        <  8:132790/149‑1 (MQ=255)
                                                                cgatgaacgcgAAGCACGCCATCCGCTGATATAACTTCAGTAGATCATCATGGAGGTCTACCTGCAGATATTTGAACT‑GCCCGGGGTCTAGGGAAA‑GATGTTACAACATATGGCCTTTACAGTCATCATGGCCCTGTTGGGCGCgagga                                                                                 >  4:241971/8‑149 (MQ=255)
                                                                 gCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatgat                                                                                <  4:134825/149‑1 (MQ=255)
                                                                      ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGCa                                                                                                                                        <  4:92982/89‑1 (MQ=18)
                                                                      ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGCa                                                                                                                                        >  3:92982/1‑89 (MQ=18)
                                                                      ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAACGATGTTCCACCCTATGGCCTTTACCGTGGTCATgg                                                                                                   <  1:176779/126‑1 (MQ=255)
                                                                      ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATgg                                                                                                   >  2:176779/1‑125 (MQ=255)
                                                                      ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCttt                                                                           >  5:189971/1‑149 (MQ=255)
                                                                       cgcgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTc                                                                          >  4:212836/1‑149 (MQ=255)
                                                                           aaGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTc                                                                      <  6:259289/149‑1 (MQ=255)
                                                                                                   ggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGcc                                              <  2:240259/149‑1 (MQ=255)
                                                                                                   ggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCACTGATGGGCGCTATGATACTTTCGGTAACGTTCGTTCAGGCAGACATTGcc                                              >  4:199679/1‑149 (MQ=21)
                                                                                                          gATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTc                                        <  7:247208/148‑1 (MQ=255)
                                                                                                            tcatcaTGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGt                                      >  4:229349/1‑148 (MQ=255)
                                                                                                                     gggtgTCCCTGCCGATCTGTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAg                            <  6:189971/148‑1 (MQ=255)
                                                                                                                            ccTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCAtt                                                 <  3:3448/121‑1 (MQ=255)
                                                                                                                            ccTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCAtt                                                 >  4:3448/1‑121 (MQ=255)
                                                                                                                            ccTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAag                      <  3:212836/148‑1 (MQ=255)
                                                                                                                                 cGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGGTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGACCTGTTCGTCACCGGCAAGGTCAaggaagag                >  7:59914/1‑149 (MQ=255)
                                                                                                                                  gATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagag                >  5:109508/1‑148 (MQ=255)
                                                                                                                                   aTCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagagg               >  3:180352/1‑148 (MQ=255)
                                                                                                                                     tcttGCCCTCGACCCCGGTC‑AGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCACGGTCAaggaagaggaag            >  1:122073/3‑149 (MQ=255)
                                                                                                                                             t‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGt     >  1:100176/1‑148 (MQ=255)
                                                                                                                                                agcGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCTCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCTCCGGCAAGGTCAAGGAGGAGGCAGGCCTGGTGAt  <  3:241971/147‑1 (MQ=255)
                                                                                                                                                aCCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCCTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGAt  <  6:241546/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCCATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGAT  >  NC_002947/2754939‑2755230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑