| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,826,825 | G→C | 12.5% | A80P (GCA→CCA) | PP_2479 → | cytochrome c family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,826,825 | 0 | G | C | 12.5% | 154.0 / 13.2 | 56 | A80P (GCA→CCA) | PP_2479 | cytochrome c family protein |
| Reads supporting (aligned to +/- strand): ref base G (22/27); new base C (5/2); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
GGGCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGC > NC_002947/2826677‑2826968 | gggCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTg > 8:72189/1‑149 (MQ=255)gggCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTg > 1:10366/1‑149 (MQ=255)gggCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTg < 6:268305/149‑1 (MQ=255) ggCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGc < 4:82179/149‑1 (MQ=255) cGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCAt < 2:10366/149‑1 (MQ=255) tACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGAc < 3:260550/149‑1 (MQ=255) tACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGAc < 6:261092/149‑1 (MQ=255) tGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt > 1:68403/1‑149 (MQ=255) gcgcgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGTCATCGGTCCATGCACCGATGCCg < 6:249049/149‑1 (MQ=255) cgcgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGa < 2:99962/149‑1 (MQ=255) gcgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAg > 6:247408/1‑149 (MQ=255) ccAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTc < 2:68403/149‑1 (MQ=255) tgtgGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTgcgc > 2:125/1‑149 (MQ=255) cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGAc > 7:165257/1‑123 (MQ=255) cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGAc < 8:165257/123‑1 (MQ=255) cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCgg > 5:61109/1‑149 (MQ=255) tACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt < 4:261770/118‑1 (MQ=255) gTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTgg < 6:61109/149‑1 (MQ=255) cTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGcc > 7:20637/1‑147 (MQ=255) cTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGcc < 8:20637/147‑1 (MQ=255) ggCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGa > 4:209048/1‑149 (MQ=255) ggTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACgg > 6:30877/1‑149 (MQ=255) cccTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCa > 3:155096/1‑149 (MQ=255) ccTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCac < 4:155096/149‑1 (MQ=255) cGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCacta < 1:125/147‑1 (MQ=255) gtggtgTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCg < 5:247408/149‑1 (MQ=255) tggtgTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCgg > 6:170637/1‑149 (MQ=255) tgtTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGt > 3:10346/1‑149 (MQ=255) ttGAGTTCAAGCTGCCGTTCGGCTAGCTGTTTTCGCCCAAGATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCTATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGttt < 3:216872/149‑1 (MQ=255) gAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc > 2:38452/1‑149 (MQ=255) gTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTccc < 2:86839/148‑1 (MQ=255) ttCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc > 1:186710/1‑146 (MQ=255) ttCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc < 2:186710/146‑1 (MQ=255) ttCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCta < 1:38452/149‑1 (MQ=255) cGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTAc < 5:30877/149‑1 (MQ=255) gCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCac > 4:15550/1‑113 (MQ=255) ttttCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTcgcg > 1:57985/1‑149 (MQ=255) cGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGa < 1:134183/148‑1 (MQ=255) cccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc < 1:268062/116‑1 (MQ=255) cccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc > 2:268062/1‑116 (MQ=255) ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa > 4:110312/1‑148 (MQ=255) aCATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATc > 5:71962/1‑149 (MQ=255) ttACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTc > 5:85915/1‑149 (MQ=255) ttACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGCCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTc < 5:170637/149‑1 (MQ=255) cccGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTcgc > 3:71571/1‑131 (MQ=255) cccGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTcgc < 4:71571/131‑1 (MQ=255) cccGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGa > 8:35659/1‑149 (MQ=255) gACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCaa < 3:209048/149‑1 (MQ=255) cccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAgg < 6:85915/149‑1 (MQ=255) ccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTGCTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAggg < 2:56525/149‑1 (MQ=255) aaCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGGCATCCTCGCGATCAAGGGTTAtc < 8:59088/149‑1 (MQ=255) aaCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtc < 6:71962/149‑1 (MQ=255) ctcgtcATCGGTCCATGCACCGATGCCGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct > 2:95823/6‑149 (MQ=255) ctcgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct > 5:196818/6‑149 (MQ=255) tcgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGAt > 1:19607/5‑122 (MQ=255) tcgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGAt < 2:19607/118‑1 (MQ=255) gtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa > 5:12718/3‑149 (MQ=255) gtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa > 5:51916/3‑149 (MQ=255) acGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATAGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTCTACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGc > 6:129555/2‑149 (MQ=255) | GGGCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGC > NC_002947/2826677‑2826968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |