Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,878,338 T→C 10.8% H223R (CAC→CGC)  PP_2533 ← D‑isomer specific 2‑hydroxyacid dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,878,3380TC10.8% 96.0 / 2.8 37H223R (CAC→CGC) PP_2533D‑isomer specific 2‑hydroxyacid dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (18/15);  new base C (2/2);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TGGATGGGCCGGGTCGAGTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCTTTGGCTGCGG  >  NC_002947/2878190‑2878470
                                                                                                                                                    |                                                                                                                                    
tGGATGGGCCGGGTCGAGTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCAtgt                                                                                                                                      <  4:110824/149‑1 (MQ=255)
  gATGGGCCGGGTCGAGTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCAtgtg                                                                                                                                     <  6:271799/148‑1 (MQ=255)
   aTGGGCCGGGTCGAGTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTgg                                                                                                                                    >  5:56819/1‑148 (MQ=255)
    tGGGCCGGGTCGAGTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGa                                                                                                                                  >  5:125386/1‑149 (MQ=255)
                 gTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAg                                                                                                                     <  3:203833/149‑1 (MQ=255)
                 gTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAg                                                                                                                     <  6:56819/149‑1 (MQ=255)
                              cTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGc                                                                                                        <  6:125386/149‑1 (MQ=255)
                               tCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCg                                                                                                       >  4:208710/1‑149 (MQ=255)
                                cATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGt                                                                                                                   <  4:243876/136‑1 (MQ=255)
                                cATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGt                                                                                                                   >  3:243876/1‑136 (MQ=255)
                                  tcaaCATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGAGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGGCGTCGAACGTCACCCCATTGCgtgt                                                                                                    <  6:51596/147‑1 (MQ=255)
                                       aTCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGtcgtcg                                                                                                                        >  8:165574/1‑124 (MQ=255)
                                       aTCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGtcgtcg                                                                                                                        <  7:165574/124‑1 (MQ=255)
                                        tCCACCTCCGCGTAGCCCGGCCTCCCGGCATCCAATGCCGGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATc                                                                                              <  6:195008/149‑1 (MQ=255)
                                          cACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATcc                                                                                             >  4:206361/1‑148 (MQ=255)
                                             cgccgcGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGaaa                                                                                         <  3:208710/149‑1 (MQ=255)
                                                     aGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAgg                                                                                 >  8:141726/1‑149 (MQ=255)
                                                           gccgccCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATc                                                                                              >  1:104931/1‑130 (MQ=255)
                                                           gccgccCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATc                                                                                              <  2:104931/130‑1 (MQ=255)
                                                             cgccCGGCATCCAATGCCTGCAGGAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTGATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCt                                                                         <  7:108666/149‑1 (MQ=255)
                                                                 cGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGAc                                                                      >  1:229480/1‑148 (MQ=255)
                                                                          aTGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCAATGGCGTGTCTGATCCGAAAGGCGCACGTTCAGGCTGACAATATCCGcc                                                            >  7:70322/1‑149 (MQ=255)
                                                                              cTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCgg                                                        >  5:276759/1‑149 (MQ=255)
                                                                                gCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGc                                                       >  3:105041/1‑148 (MQ=255)
                                                                                                       aGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAggcggc                               <  4:105041/149‑1 (MQ=255)
                                                                                                             gcgcgGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAat                                                                   <  4:235552/107‑1 (MQ=255)
                                                                                                             gcgcgGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAat                                                                   >  3:235552/1‑107 (MQ=255)
                                                                                                             gcgcgGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCagcagc                         <  2:229480/149‑1 (MQ=255)
                                                                                                                cgGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGtt                      <  8:70322/149‑2 (MQ=255)
                                                                                                                     gACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCGTGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCAt                 >  4:64532/1‑149 (MQ=255)
                                                                                                                      aCGTTCACCAGCAAGGCGTCGGGCTTCATGCGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATc                >  2:23544/1‑149 (MQ=255)
                                                                                                                      aCGTTCACCAGCAAGGCGTCGGGCTTCATGCGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATc                >  2:19343/1‑149 (MQ=255)
                                                                                                                        gTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCgg              >  7:218025/1‑149 (MQ=255)
                                                                                                                            aCCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCttt          >  4:64423/1‑149 (MQ=255)
                                                                                                                            aCCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCtt           <  7:141726/148‑1 (MQ=255)
                                                                                                                            aCCAGCAAGGCGTCGGGCTTCATGCGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCttt          <  1:23544/149‑1 (MQ=255)
                                                                                                                            aCCAGCAAGGCGTCGGGCTTCATGCGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCttt          <  1:19343/149‑1 (MQ=255)
                                                                                                                                    ggCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCTTTGGCTgcgg  >  6:173397/1‑149 (MQ=255)
                                                                                                                                    ggCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCTTTGGCTgcgg  >  7:62359/1‑149 (MQ=255)
                                                                                                                                                  tgtgGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAatat                                                                 <  4:155995/72‑1 (MQ=255)
                                                                                                                                                  tgtgGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAatat                                                                 >  3:155995/1‑72 (MQ=255)
                                                                                                                                                    |                                                                                                                                    
TGGATGGGCCGGGTCGAGTAACGGCTCTTCCTCATAAACATCCACCGCCGCGTAGCCCGGCCGCCCGGCATCCAATGCCTGCAGTAATGCGCCTGGTGCGATCAGTTCCGCGCGGCTGACGTTCACCAGCAAGGCGTCGGGCTTCATGTGGGACAGGTCGTCGAAGGTCACCCCATGGCGTGTCTGATCCGAAAGGCGCAGGTTCAGGCTGACAATATCCGCCTCGGCAAAAAACGCCTCACGCGAGGCGGCAGCAGCGTGCCCATCGGCTTTGGCTGCGG  >  NC_002947/2878190‑2878470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑