| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,941,698 | G→T | 7.0% | intergenic (+18/+28) | PP_2574 → / ← PP_2575 | LysR family transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,941,698 | 0 | G | T | 7.0% | 180.2 / 4.0 | 57 | intergenic (+18/+28) | PP_2574/PP_2575 | LysR family transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (23/30); new base T (2/2); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
TCGACATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCGCGCCGGTCCTGGCGCCGGTCCTGACGCTGGCGCAT > NC_002947/2941553‑2941845 | tCGACATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCgg > 2:155321/1‑147 (MQ=255) gACATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCa < 1:155321/147‑1 (MQ=255) gACATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAg > 1:210363/1‑148 (MQ=255) ccGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAg < 1:236452/141‑1 (MQ=255) ccGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAg > 2:236452/1‑141 (MQ=255) gTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCAACCCGGCAGGAGTAGCAtggcgtgg > 8:196595/1‑149 (MQ=255) tCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCt > 1:29093/1‑148 (MQ=255) ggggCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAg > 2:231326/1‑149 (MQ=255) ggctggctGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAggg < 2:29093/149‑1 (MQ=255) ggctggctGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAggg < 8:119108/149‑1 (MQ=255) ggctgCGGCGTGGTTGAAGCGAGTCCAGCTGACCACACCGGCCCAGTTGGTCGTGGAGTTTTGTCTGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACACCGGCAGGAGTAGCATGGCGTGTCGATTGCG‑CAtgggat > 7:85899/1‑146 (MQ=255) gctgCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGa > 2:72479/1‑149 (MQ=255) cgTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTa < 5:34665/149‑1 (MQ=255) tGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGc < 3:98534/149‑1 (MQ=255) ttGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCcgc > 5:234349/1‑149 (MQ=255) tGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCcgcg > 6:150131/1‑149 (MQ=255) aCCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACGTgg < 8:194573/124‑1 (MQ=255) aCCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTgg > 7:194573/1‑124 (MQ=255) aCCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCCGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGc > 4:22817/1‑149 (MQ=255) ccAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCt < 6:100335/149‑1 (MQ=255) aaaCCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGt < 1:72479/149‑1 (MQ=255) aaaCCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCCGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGt < 3:22817/149‑1 (MQ=255) aCCGGCGCAGTTGTTCGGGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAg < 7:219382/149‑1 (MQ=255) cAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTgg < 2:210363/149‑1 (MQ=255) aGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCTACTCCTGCCGGGGTGGCAtg < 3:127585/82‑1 (MQ=25) aGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCTACTCCTGCCGGGGTGGCAtg > 4:127585/1‑82 (MQ=25) aGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTgg > 1:47264/1‑148 (MQ=255) aGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGc < 8:50253/149‑1 (MQ=255) ttgttCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCTCCAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCa < 1:206194/149‑1 (MQ=255) ttCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATgg < 7:80312/148‑1 (MQ=255) cGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTgg < 3:212949/141‑1 (MQ=255) cGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTgg > 4:212949/1‑141 (MQ=255) ggAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAggg < 6:235415/91‑1 (MQ=255) ggAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAggg > 5:235415/1‑91 (MQ=255) gAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTggg > 2:26416/1‑149 (MQ=255) gTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGtt > 4:146429/1‑149 (MQ=255) ttttGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAg < 4:135399/127‑1 (MQ=255) ttttGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAg > 3:135399/1‑127 (MQ=255) ttGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGtttt < 7:196595/148‑1 (MQ=255) tGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTg < 2:47264/148‑1 (MQ=255) tGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTg < 3:146429/148‑1 (MQ=255) tGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGAAGGCACTGGCATGGGCCTGGGTTTTgg < 1:231326/149‑1 (MQ=255) tCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGcc < 5:150131/149‑1 (MQ=255) gggAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTc < 6:234349/149‑1 (MQ=255) aaGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTc > 8:88949/1‑149 (MQ=255) gCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGtttt < 7:107631/132‑1 (MQ=255) gCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGtttt > 8:107631/1‑132 (MQ=255) tGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCgcgccggtcctg > 6:118647/1‑149 (MQ=255) aGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCgcgccggtcctggcgccgg > 4:254524/1‑149 (MQ=255) aGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGCTTTGGCCTCGTCCTGGTCgcgccggtcctggcgccg < 7:88949/148‑1 (MQ=255) gCCATGCCACCCCGGCAGGAGTTGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCgcgccggtcctggcgccggtcctg < 5:215321/149‑1 (MQ=255) ccATGCTACTCCTGCCGGGGTGGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTg < 1:54425/110‑1 (MQ=38) ccATGCTACTCCTGCCGGGGTGGCATGGCGTGGCGATTGCT‑AAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCCCTGTAGGCACTGGCATGGGCCAGGCTTTTg > 2:54425/1‑110 (MQ=25) cATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCgcgccggtcctggcgccg < 2:31286/141‑1 (MQ=255) cATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCgcgccggtcctggcgccg > 1:31286/1‑141 (MQ=255) ccccGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCGCGCCGGTCCTGGCGCCGGTCCTGACGCTGgcg < 2:160161/149‑1 (MQ=255) ccGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCGCGCCGGTCCTGGCGCCGGTCCTGACGCTGGCGCa > 5:256911/1‑149 (MQ=255) cGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCGCGCCGGTCCTGGCGCCGGTCCTGACGCTGGCGCAt < 1:26416/149‑1 (MQ=255) | TCGACATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATGCCACCCCGGCAGGAGTAGCATGGCGTGGCGATTGCT‑CAGGGGTTGACCTGGTAGCCGCGCCCTTGCAGGCAACCGCTGTAGGCACTGGCATGGGCCTGGGTTTTGGCCTCGTCCTGGTCGCGCCGGTCCTGGCGCCGGTCCTGACGCTGGCGCAT > NC_002947/2941553‑2941845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |