Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,981,152 T→C 9.3% K405E (AAG→GAG)  PP_2607 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,981,1520TC 9.3% 116.0 / 2.6 43K405E (AAG→GAG) PP_2607hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/21);  new base C (2/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGCATCGCTGTTGAACTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAGCGCCGCCCGGTTTGG  >  NC_002947/2981015‑2981294
                                                                                                                                         |                                                                                                                                              
gCCGGCATCGCTGTTGAACTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCgcgc                                                                                                                                       >  1:49213/1‑147 (MQ=255)
          cTGTTGAACTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTCGGGCCGCGCCAGGCCAAGccc                                                                                                                           >  5:54710/1‑149 (MQ=255)
          cTGTTGAACTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTCGGGCCGCGCCAGGCCAAGccc                                                                                                                           >  5:58585/1‑149 (MQ=255)
               gAACTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAg                                                                                                                       <  7:55565/148‑1 (MQ=255)
                  cTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGt                                                                                                                   >  5:221852/1‑149 (MQ=255)
                     tgAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCa                                                                                                                >  1:4955/1‑149 (MQ=255)
                         aGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGc                                                                                                            <  2:49213/149‑1 (MQ=255)
                            gtTTGCTCAATCTGCTGTGTATCGCCGCAGATGGCCAGTGCGAGTGGGTGGGATGTGGTGGCCCGGTAGAGGTAGCCTGCGTGGATCAACGCCTGGTTGTTGACGGGCTTTGGCCGCGCCAGGCGAAGCCCGTAGCGGTTCATTGCCt                                                                                                          <  4:112326/148‑1 (MQ=255)
                             tttGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTc                                                                                                         <  3:87207/148‑1 (MQ=255)
                                 cgcAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCAt                                                                                                    <  2:87909/149‑1 (MQ=255)
                                  gcAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATc                                                                                                   >  3:64084/1‑149 (MQ=255)
                                     aaCAGCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTCGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCa                                                                                                <  6:54710/149‑1 (MQ=255)
                                     aaCACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTCGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCa                                                                                                <  6:58585/149‑1 (MQ=255)
                                          cTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGAc                                                                                             >  1:155510/1‑147 (MQ=255)
                                            gtgGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGAccagc                                                                                         >  3:11202/1‑149 (MQ=255)
                                                 tCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGCccagccag                                                                                      >  2:176263/1‑147 (MQ=255)
                                                  cGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCcagcag                                                                                   >  2:191609/1‑149 (MQ=255)
                                                        cGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGc                                                                              >  4:91821/1‑148 (MQ=255)
                                                                agggccagTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGGTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCttt                                                                     <  4:64084/149‑1 (MQ=255)
                                                                agggccagTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGCTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCttt                                                                     <  3:124430/149‑1 (MQ=255)
                                                                  ggccagTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTgg                                                                   >  2:179023/1‑149 (MQ=255)
                                                                   gccagTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGc                                                                  <  7:146283/149‑1 (MQ=255)
                                                                            tGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTgg                                                                   <  8:82225/139‑1 (MQ=255)
                                                                            tGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTgg                                                                   >  7:82225/1‑139 (MQ=255)
                                                                              cGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATtgtgt                                                       <  1:179023/149‑1 (MQ=255)
                                                                                aTGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGc                                                     <  4:11202/149‑1 (MQ=255)
                                                                                         cccGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAgtgg                                            <  2:4955/149‑1 (MQ=255)
                                                                                                    tAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTc                                 <  3:91821/149‑1 (MQ=255)
                                                                                                        cTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGAc                             <  1:176263/149‑1 (MQ=255)
                                                                                                            gTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATtgtgt                                                       <  7:111402/119‑1 (MQ=255)
                                                                                                            gTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATtgtgt                                                       >  8:111402/1‑119 (MQ=255)
                                                                                                            gTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGa                              >  6:219336/1‑144 (MQ=255)
                                                                                                            gTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGa                              <  5:219336/144‑1 (MQ=255)
                                                                                                                    aCGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTa                                       >  7:100542/1‑127 (MQ=255)
                                                                                                                    aCGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTa                                       <  8:100542/127‑1 (MQ=255)
                                                                                                                    aCGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAg                 <  2:155510/149‑1 (MQ=255)
                                                                                                                     cGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAgc                >  7:120489/1‑149 (MQ=255)
                                                                                                                         tGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAGcgccg            >  7:250539/1‑149 (MQ=255)
                                                                                                                                 gACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAGCGCCGCCCGGttt    >  3:18361/1‑149 (MQ=255)
                                                                                                                                  aCGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAATTGGTGGTAGGAGTCGGACTCGTTGACGAAGCGCCGCCCGGTTTg   <  1:191609/149‑1 (MQ=255)
                                                                                                                                   cGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAGCGCCGCCCGGTTTgg  <  6:221852/149‑1 (MQ=255)
                                                                                                                                       cTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAgcg               <  7:153092/132‑1 (MQ=255)
                                                                                                                                       cTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAgcg               >  8:153092/1‑132 (MQ=255)
                                                                                                                                         |                                                                                                                                              
GCCGGCATCGCTGTTGAACTGTGAAAGTGTTTGCGCAAACACCTGTGGATCGACGCCGATGGCCAGGGCCAGTGCCTGCGATGTGGTGGCCCGGTACAGGTAGCCTGCGTCGATCAACGCCTGGTTGTTGACGGGCTTGGGCCGCGCCAGGCCAAGCCCGTAGCGGTTCATTGCCTCGGCATCGCAGACCAGCCAGCAGCTGGCAATGCCTTTGGCGAACATTGTGTGCACGAAGTGGTGGTAGGAGTCGGACTCGTTGACGAAGCGCCGCCCGGTTTGG  >  NC_002947/2981015‑2981294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑