Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,008,885 C→T 11.4% W70* (TGG→TGA)  PP_2628 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,008,8850CT11.4% 87.2 / 4.1 35W70* (TGG→TGA) PP_2628ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (17/14);  new base T (2/2);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CCTGCCAGGACGCGAGCAGGTCGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCTTGCCTTTGCCGACT  >  NC_002947/3008758‑3009032
                                                                                                                               |                                                                                                                                                   
cctgccAGGACGCGAGCAGGTCGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGccc                                                                                                                                                    >  1:144473/1‑129 (MQ=255)
cctgccAGGACGCGAGCAGGTCGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGccc                                                                                                                                                    <  2:144473/129‑1 (MQ=255)
    ccAGGACGCGAGCAGGTCGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACg                                                                                                                             <  5:9658/148‑1 (MQ=255)
      aGGACGCGAGCAGGTCGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGggc                                                                                                                          >  4:222560/1‑149 (MQ=255)
                     cGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCtcg                                                                                                           >  7:183621/1‑149 (MQ=255)
                           ccTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGt                                                                                                     >  6:81490/1‑149 (MQ=255)
                                 ccAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCcaca                                                                                                                                                 <  4:230067/99‑1 (MQ=255)
                                 ccAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCcaca                                                                                                                                                 >  3:230067/1‑99 (MQ=255)
                                   aGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAggg                                                                                             <  2:65473/149‑1 (MQ=255)
                                        cgcgAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGAAAGGGGTCGa                                                                                        <  5:125517/149‑1 (MQ=255)
                                        cgcgAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCCGTCGTACCTCGTCGGGTGGGAAGGGGTc                                                                                          >  4:99860/1‑147 (MQ=255)
                                          cgAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGc                                                                                      <  5:81490/149‑1 (MQ=255)
                                           gAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCTCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGcc                                                                                     >  3:192313/1‑149 (MQ=255)
                                           gAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCTCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGcc                                                                                     >  3:195796/1‑149 (MQ=255)
                                           gAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGcc                                                                                     >  1:58679/1‑149 (MQ=255)
                                           gAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGcc                                                                                     <  3:222560/149‑1 (MQ=255)
                                                     gCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCaaa                                                                           <  2:58679/149‑1 (MQ=255)
                                                          gTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATAc                                                                      >  1:110326/1‑149 (MQ=255)
                                                                     ccccGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCTCACATGAAGCGCATCAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCtt                                                           <  4:192313/149‑1 (MQ=255)
                                                                     ccccGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCTCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCtt                                                           <  4:195796/149‑1 (MQ=255)
                                                                      cccGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTg                                                          >  4:200034/1‑149 (MQ=255)
                                                                       ccGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGt                                                         >  4:222684/1‑149 (MQ=255)
                                                                       ccGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGt                                                         <  8:178069/149‑1 (MQ=255)
                                                                       ccGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGt                                                         >  7:178069/1‑149 (MQ=255)
                                                                            aCTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCtcgtc                                                                                                         >  1:15709/1‑96 (MQ=255)
                                                                            aCTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCtcgtc                                                                                                         <  2:15709/96‑1 (MQ=255)
                                                                                    gcgccagcgccaGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACgg                                            >  8:71321/1‑149 (MQ=255)
                                                                                    gcgccagcgccaGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACgg                                            <  7:71321/149‑1 (MQ=255)
                                                                                            gccagGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTcttgct                                    >  4:230209/1‑149 (MQ=255)
                                                                                                            gggCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGcc                    >  5:178716/1‑149 (MQ=255)
                                                                                                              gcgcCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTcttgcttg                                  >  3:82666/1‑133 (MQ=255)
                                                                                                              gcgcCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTcttgcttg                                  <  4:82666/133‑1 (MQ=255)
                                                                                                                 ccgcGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGTCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTACGGAAAACGGTTCTTGATTGGCCAATGCCCTGCCTGCtt               >  2:93756/1‑149 (MQ=255)
                                                                                                                 ccgcGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCtt               <  3:230209/149‑1 (MQ=255)
                                                                                                                       gTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCTTGCCttt         <  8:183621/149‑1 (MQ=255)
                                                                                                                         aCAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCTTGCCTTTGc       >  6:227168/1‑149 (MQ=255)
                                                                                                                          cAAGCCCACATGAAGCGAAACCGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGGCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCTTGCCTTTGcc      <  1:93756/149‑1 (MQ=255)
                                                                                                                              cccACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCTTGGCTTTGcggccg  >  4:159024/1‑144 (MQ=255)
                                                                                                                               |                                                                                                                                                   
CCTGCCAGGACGCGAGCAGGTCGACCACCTGGCCCAGGAACGCGAATAGCCAGGCTTCGTATATCGACACCCCGGCACTGAGCAGCGCCAGCGCCAGGATGTAGCCACGGGCGCCGCGGGTACAAGCCCACATGAAGCGCAACAGGCCAACGGGCGGCGGTGGTACCTCGTCGGGTGGGAAGGGGTCGAGCCGACGTTCAAAGATACGCAGCATGCTTGTCTCCGGAAAACGGTTCTTGCTTGGCCAATGCCCTGCCGGCTTGCCTTTGCCGACT  >  NC_002947/3008758‑3009032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑