Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,011,111 T→A 6.9% W58R (TGG→AGG) ‡ bcsG → endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,011,1110TA 6.9% 171.8 / 2.6 58W58R (TGG→AGG) ‡bcsGendoglucanase
Reads supporting (aligned to +/- strand):  ref base T (27/26);  new base A (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTATCTGCTCGAGCTGCTGGGCCG  >  NC_002947/3010966‑3011259
                                                                                                                                                 |                                                                                                                                                     
ggTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGc                                                                                                                                                   >  3:47352/1‑149 (MQ=255)
       gCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTa                                                                                                                                             <  2:8065/148‑1 (MQ=255)
       gCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTAt                                                                                                                                            >  4:74125/1‑149 (MQ=255)
       gCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTAt                                                                                                                                            >  1:251394/1‑149 (MQ=255)
                 cTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCaa                                                                                                                                  <  3:74125/149‑1 (MQ=255)
                   ggACTGGGAGGGTGGAACCTCTCTTTTTTTGCAAAGGTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCCGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAAct                                                                                                                                <  8:253998/149‑1 (MQ=255)
                         ggAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGc                                                                                                                          >  6:198772/1‑149 (MQ=255)
                         ggAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGc                                                                                                                          >  2:187823/1‑149 (MQ=255)
                          gAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTAGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCg                                                                                                                         >  8:57541/1‑149 (MQ=255)
                            gggTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGt                                                                                                                       <  7:73333/149‑1 (MQ=255)
                            gggTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGt                                                                                                                       >  7:232817/1‑149 (MQ=255)
                              gTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGc                                                                                                                     >  8:242179/1‑149 (MQ=255)
                               tGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGcc                                                                                                                    <  4:47352/149‑1 (MQ=255)
                                ggAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTTTTCGATGCGTTTCTGCTTGTCCCTTTGCCGTGCAAGG‑TGCTGCGTATTGACCGGCAACTGATGGAGGTGca                                                                                                                    >  8:111932/1‑147 (MQ=255)
                                    ccTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCg                                                                                                               <  2:251394/149‑1 (MQ=255)
                                       ctATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCaa                                                                                                                                  >  8:196410/1‑127 (MQ=255)
                                       ctATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCaa                                                                                                                                  <  7:196410/127‑1 (MQ=255)
                                        tATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTAt                                                                                                           >  5:102283/1‑149 (MQ=255)
                                          tttttttGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTCGGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTa                                                                                                            <  1:187823/147‑1 (MQ=255)
                                                  cAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCtttt                                                                                                  <  6:16934/148‑1 (MQ=255)
                                                  cAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCtttt                                                                                                  <  5:198772/148‑1 (MQ=255)
                                                       tttCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAATA‑CGCAGCCACTTGCCCGGCaaagg                                                                                                                               >  3:260500/1‑111 (MQ=37)
                                                       tttCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAATA‑CGCAGCCACTTGCCCGGCaaagg                                                                                                                               <  4:260500/114‑4 (MQ=37)
                                                         tCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAg                                                                                           <  5:273623/148‑1 (MQ=255)
                                                              tgGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACAcc                                                                                     >  5:141120/1‑149 (MQ=255)
                                                                gTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTg                                                                                   >  4:120452/1‑149 (MQ=255)
                                                                     gcTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACctgcctgc                                                                              >  8:7067/1‑149 (MQ=255)
                                                                          gCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCAccc                                                                         <  8:232817/149‑1 (MQ=255)
                                                                               cTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAg                                                                    <  7:57541/149‑1 (MQ=255)
                                                                                tCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCg                                                                                                               <  8:169620/105‑1 (MQ=255)
                                                                                tCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCg                                                                                                               >  7:169620/1‑105 (MQ=255)
                                                                                     ttCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAATA‑CGCAGCCACTTGCCCGGCaaa                                                                                                                                 >  1:123346/1‑81 (MQ=21)
                                                                                     ttCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAATA‑CGCAGCCACTTGCCCGGCaaa                                                                                                                                 <  2:123346/82‑2 (MQ=21)
                                                                                     ttCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCAc                                                                           >  1:33834/1‑136 (MQ=255)
                                                                                     ttCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCAc                                                                           <  2:33834/136‑1 (MQ=255)
                                                                                     ttCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCct                                                              >  5:29369/1‑149 (MQ=255)
                                                                                       ccAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCct                                                              <  5:114535/147‑1 (MQ=255)
                                                                                        cAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGc                                                                              >  1:33749/1‑130 (MQ=255)
                                                                                        cAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGc                                                                              <  2:33749/130‑1 (MQ=255)
                                                                                        cAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCctgc                                                            <  7:7067/148‑1 (MQ=255)
                                                                                                   gAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCgg                                                >  1:171388/1‑149 (MQ=255)
                                                                                                   gAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCgg                                                >  7:130100/1‑149 (MQ=255)
                                                                                                      ccTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCggggg                                             >  4:30786/1‑149 (MQ=255)
                                                                                                       cTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCt                                                                                >  7:104119/1‑113 (MQ=255)
                                                                                                       cTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCt                                                                                <  8:104119/113‑1 (MQ=255)
                                                                                                               cGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGAc                                     <  3:120452/148‑1 (MQ=255)
                                                                                                               cGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGAc                                     <  6:102283/148‑1 (MQ=255)
                                                                                                               cGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACt                                    >  1:138662/1‑149 (MQ=255)
                                                                                                                  tGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCt                                                                                <  4:179203/102‑1 (MQ=255)
                                                                                                                  tGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTtct                                 >  6:214025/1‑149 (MQ=255)
                                                                                                                   gCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCTTATTGCCCGGCAACTGCTGGCGGGGCCAATCGTTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTtctc                                <  7:111932/149‑1 (MQ=255)
                                                                                                                     gTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTTTCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGt                              >  6:149495/1‑149 (MQ=255)
                                                                                                                           gCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAAACGGTATCGCGCTTTTCTACCAGCACACCTCGCTCCCACCCTACAGCCGCCTGCTGGCGCAGCCGGGGCTGCTGGACTTCTCGTGGGAc                         >  6:85182/1‑148 (MQ=255)
                                                                                                                               gTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTAt                      <  6:141120/147‑1 (MQ=255)
                                                                                                                               gTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTAt                      <  5:214025/147‑1 (MQ=255)
                                                                                                                               gTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTATCt                    >  5:12466/1‑149 (MQ=255)
                                                                                                                                   cTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACt                        >  1:108200/1‑141 (MQ=255)
                                                                                                                                           ggCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTATCTGCTCGAgctgct        <  8:130100/149‑1 (MQ=255)
                                                                                                                                                 t‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTATCTGCTCGAGCTGCTGGGCCg  >  2:51277/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGT‑GGCTGCGTATTGCCCGGCAACTGCTGGCGGTGCCAATCGGTATCGCGCTTTTCTACCAGGACACCTGGCTGCCACCCTTCAGCCGCCTGCTGGCGCAGCCGGGGGTGCTGGACTTCTCGTGGGACTATCTGCTCGAGCTGCTGGGCCG  >  NC_002947/3010966‑3011259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑