Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,057,283 A→G 8.1% L22P (CTG→CCG) ‡ PP_2669 ← outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,057,2830AG 8.1% 138.0 / 2.8 49L22P (CTG→CCG) ‡PP_2669outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base G (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

TGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTC  >  NC_002947/3057137‑3057431
                                                                                                                                                  |                                                                                                                                                      
tGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAgc                                                                                                                                                      <  8:178591/149‑1 (MQ=255)
       tgACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAggg                                                                                                                                               >  5:69431/1‑149 (MQ=255)
        gACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                 <  6:86868/146‑1 (MQ=255)
        gACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                 >  5:86868/1‑146 (MQ=255)
        gACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgc                                                                                                                                              >  2:185691/1‑149 (MQ=255)
                caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgcgc                                                                                                                                            >  4:238584/1‑143 (MQ=255)
                caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgcgc                                                                                                                                            <  3:238584/143‑1 (MQ=255)
                caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGAca                                                                                                                                      <  7:127325/149‑1 (MQ=255)
                   gCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGTCGGGCGCCCTGGCGCTGGCcagca                                                                                                                                   <  6:28377/149‑1 (MQ=255)
                            gcgcTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGt                                                                                                                          <  1:185691/149‑1 (MQ=255)
                                  gCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTaacg                                                                                                                      >  4:181176/1‑145 (MQ=255)
                                     cACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAg‑gg                                                                                                                >  5:151932/1‑149 (MQ=255)
                                       ccGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGcc                                                                                                              >  3:207114/1‑149 (MQ=255)
                                        cGGCAGGGTTTCGGTCACTTCCAGGGTATGCAGGTCGATAAGACTGAGGCTGATGTCCTTATCGATGGAGACGCAGGCGGTCGAAGCGATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCACGATGTGCTCAAGCAG‑GGccc                                                                                                             >  8:201413/1‑149 (MQ=255)
                                         ggCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCg                                                                                                            <  4:133041/149‑1 (MQ=255)
                                          gCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                 <  2:103720/112‑1 (MQ=255)
                                          gCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                 >  1:103720/1‑112 (MQ=255)
                                            aGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTg                                                                                                         <  2:38040/149‑1 (MQ=255)
                                                   cGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgc                                                                                                                                              >  6:232013/1‑106 (MQ=255)
                                                   cGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgc                                                                                                                                              <  5:232013/106‑1 (MQ=255)
                                                    ggTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAggg                                                                                                 >  4:231821/1‑149 (MQ=255)
                                                    ggTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAgg                                                                                                  <  4:207114/148‑1 (MQ=255)
                                                        aCTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcg                                                                                             >  6:232795/1‑149 (MQ=255)
                                                          ttCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcggc                                                                                           >  4:20604/1‑149 (MQ=255)
                                                            cccgCGTTTGCAGGGCGATCAGGCGGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGGGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcggc                                                                                           <  4:255548/144‑1 (MQ=255)
                                                             cAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAag                                                                                                     >  6:235945/1‑136 (MQ=255)
                                                             cAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAag                                                                                                     <  5:235945/136‑1 (MQ=255)
                                                                      cAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGAtt                                                                              >  7:213935/1‑149 (MQ=255)
                                                                        ggTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTcc                                                                            <  5:232795/149‑1 (MQ=255)
                                                                         gTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAgg                                                                                                                                                >  7:220410/1‑82 (MQ=255)
                                                                         gTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCt                                                                           <  2:184409/149‑1 (MQ=255)
                                                                            gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                         <  6:151932/148‑1 (MQ=255)
                                                                            gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                         <  3:181176/148‑1 (MQ=255)
                                                                              tCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                         <  8:180240/146‑1 (MQ=255)
                                                                              tCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                         >  7:180240/1‑146 (MQ=255)
                                                                              tCAGGCTGAGGCCGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTAttg                                                                      <  3:172212/149‑1 (MQ=255)
                                                                                                    ctcGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAgggg                                                 <  3:231821/148‑1 (MQ=255)
                                                                                                       gTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGAGTCCTTATTGTTGGGGTTGTCGGGTCAGGGGTAGc                                             >  6:231028/1‑149 (MQ=255)
                                                                                                                ccAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCT‑GGGTAAAGCAG‑TGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                                   >  2:99774/1‑149 (MQ=255)
                                                                                                                ccAGGCGCTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGGATGG‑CGGAGGGCTTATTGTTGTGGTAGGCGGGGCAGGGGTAGCTACACGTCa                                    >  2:87232/1‑149 (MQ=255)
                                                                                                                 cAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                                   >  5:35732/1‑149 (MQ=255)
                                                                                                                      ggggggaGCCATGGTGTGTCTGCTTGCCAGGGCCAGGGCGGCCGACATCACGGTGGGGGAAAGGCG‑GGCCCGGTGGAAGATGGGGCGGCGGATGGTCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCg                               <  1:205401/143‑1 (MQ=255)
                                                                                                                             gCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTc                       <  8:213935/149‑1 (MQ=255)
                                                                                                                                  ccccagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑ATAGTTCCTATGAGGTGTGGGTGTGGGGTGAGGGGTAGCGACACGTCACATCGGGCGTGTGATAGc                  >  1:222141/8‑149 (MQ=12)
                                                                                                                                     cagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑c                                                                                   >  1:23820/5‑81 (MQ=17)
                                                                                                                                     cagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑c                                                                                   <  2:23820/77‑1 (MQ=17)
                                                                                                                                        aCTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGCCGCTTGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAgg             <  1:87232/149‑1 (MQ=255)
                                                                                                                                                ccAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCAtt    <  5:139688/149‑1 (MQ=255)
                                                                                                                                                 cAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCAtt    >  2:75592/1‑148 (MQ=255)
                                                                                                                                                  aGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTc  >  2:3550/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTC  >  NC_002947/3057137‑3057431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑