Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,057,290 G→T 8.9% L20M (CTG→ATG) ‡ PP_2669 ← outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,057,2900GT 8.9% 137.8 / 4.2 45L20M (CTG→ATG) ‡PP_2669outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

TGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAG  >  NC_002947/3057144‑3057438
                                                                                                                                                  |                                                                                                                                                      
tgACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAggg                                                                                                                                                      >  5:69431/1‑149 (MQ=255)
 gACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                        <  6:86868/146‑1 (MQ=255)
 gACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                        >  5:86868/1‑146 (MQ=255)
 gACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgc                                                                                                                                                     >  2:185691/1‑149 (MQ=255)
         caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgcgc                                                                                                                                                   >  4:238584/1‑143 (MQ=255)
         caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgcgc                                                                                                                                                   <  3:238584/143‑1 (MQ=255)
         caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGAca                                                                                                                                             <  7:127325/149‑1 (MQ=255)
            gCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGTCGGGCGCCCTGGCGCTGGCcagca                                                                                                                                          <  6:28377/149‑1 (MQ=255)
                     gcgcTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGt                                                                                                                                 <  1:185691/149‑1 (MQ=255)
                           gCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTaacg                                                                                                                             >  4:181176/1‑145 (MQ=255)
                              cACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAg‑gg                                                                                                                       >  5:151932/1‑149 (MQ=255)
                                ccGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGcc                                                                                                                     >  3:207114/1‑149 (MQ=255)
                                 cGGCAGGGTTTCGGTCACTTCCAGGGTATGCAGGTCGATAAGACTGAGGCTGATGTCCTTATCGATGGAGACGCAGGCGGTCGAAGCGATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCACGATGTGCTCAAGCAG‑GGccc                                                                                                                    >  8:201413/1‑149 (MQ=255)
                                  ggCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCg                                                                                                                   <  4:133041/149‑1 (MQ=255)
                                   gCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                        >  1:103720/1‑112 (MQ=255)
                                   gCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAg                                                                                                                                                        <  2:103720/112‑1 (MQ=255)
                                     aGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTg                                                                                                                <  2:38040/149‑1 (MQ=255)
                                            cGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgc                                                                                                                                                     >  6:232013/1‑106 (MQ=255)
                                            cGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGgc                                                                                                                                                     <  5:232013/106‑1 (MQ=255)
                                             ggTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAggg                                                                                                        >  4:231821/1‑149 (MQ=255)
                                             ggTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAgg                                                                                                         <  4:207114/148‑1 (MQ=255)
                                                 aCTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcg                                                                                                    >  6:232795/1‑149 (MQ=255)
                                                   ttCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcggc                                                                                                  >  4:20604/1‑149 (MQ=255)
                                                     cccgCGTTTGCAGGGCGATCAGGCGGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGGGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcggc                                                                                                  <  4:255548/144‑1 (MQ=255)
                                                      cAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAag                                                                                                            >  6:235945/1‑136 (MQ=255)
                                                      cAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAag                                                                                                            <  5:235945/136‑1 (MQ=255)
                                                               cAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGAtt                                                                                     >  7:213935/1‑149 (MQ=255)
                                                                 ggTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTcc                                                                                   <  5:232795/149‑1 (MQ=255)
                                                                  gTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAgg                                                                                                                                                       >  7:220410/1‑82 (MQ=255)
                                                                  gTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCt                                                                                  <  2:184409/149‑1 (MQ=255)
                                                                     gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                <  3:181176/148‑1 (MQ=255)
                                                                     gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                <  6:151932/148‑1 (MQ=255)
                                                                       tCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                <  8:180240/146‑1 (MQ=255)
                                                                       tCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                >  7:180240/1‑146 (MQ=255)
                                                                       tCAGGCTGAGGCCGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTAttg                                                                             <  3:172212/149‑1 (MQ=255)
                                                                                             ctcGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAgggg                                                        <  3:231821/148‑1 (MQ=255)
                                                                                                gTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGAGTCCTTATTGTTGGGGTTGTCGGGTCAGGGGTAGc                                                    >  6:231028/1‑149 (MQ=255)
                                                                                                         ccAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCT‑GGGTAAAGCAG‑TGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                                          >  2:99774/1‑149 (MQ=255)
                                                                                                         ccAGGCGCTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGGATGG‑CGGAGGGCTTATTGTTGTGGTAGGCGGGGCAGGGGTAGCTACACGTCa                                           >  2:87232/1‑149 (MQ=255)
                                                                                                          cAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                                          >  5:35732/1‑149 (MQ=255)
                                                                                                               ggggggaGCCATGGTGTGTCTGCTTGCCAGGGCCAGGGCGGCCGACATCACGGTGGGGGAAAGGCG‑GGCCCGGTGGAAGATGGGGCGGCGGATGGTCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCg                                      <  1:205401/143‑1 (MQ=255)
                                                                                                                      gCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTc                              <  8:213935/149‑1 (MQ=255)
                                                                                                                           ccccagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑ATAGTTCCTATGAGGTGTGGGTGTGGGGTGAGGGGTAGCGACACGTCACATCGGGCGTGTGATAGc                         >  1:222141/8‑149 (MQ=12)
                                                                                                                              cagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑c                                                                                          >  1:23820/5‑81 (MQ=17)
                                                                                                                              cagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑c                                                                                          <  2:23820/77‑1 (MQ=17)
                                                                                                                                 aCTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGCCGCTTGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAgg                    <  1:87232/149‑1 (MQ=255)
                                                                                                                                         ccAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCAtt           <  5:139688/149‑1 (MQ=255)
                                                                                                                                          cAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCAtt           >  2:75592/1‑148 (MQ=255)
                                                                                                                                           aGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTc         >  2:3550/1‑149 (MQ=255)
                                                                                                                                              gcCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGtt      <  3:20604/149‑1 (MQ=255)
                                                                                                                                              gcCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGtt      >  7:57587/1‑149 (MQ=255)
                                                                                                                                                  gggCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAg  <  6:69431/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAG  >  NC_002947/3057144‑3057438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑