Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,057,299 A→C 9.6% S17A (TCG→GCG) ‡ PP_2669 ← outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,057,2990AC 9.6% 117.1 / 5.6 42S17A (TCG→GCG) ‡PP_2669outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (17/21);  new base C (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

CAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAGGGAT  >  NC_002947/3057153‑3057442
                                                                                                                                                  |                                                                                                                                                 
caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGAca                                                                                                                                                 <  7:127325/149‑1 (MQ=255)
   gCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGTCGGGCGCCCTGGCGCTGGCcagca                                                                                                                                              <  6:28377/149‑1 (MQ=255)
            gcgcTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGt                                                                                                                                     <  1:185691/149‑1 (MQ=255)
                  gCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTaacg                                                                                                                                 >  4:181176/1‑145 (MQ=255)
                     cACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAg‑gg                                                                                                                           >  5:151932/1‑149 (MQ=255)
                       ccGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGcc                                                                                                                         >  3:207114/1‑149 (MQ=255)
                        cGGCAGGGTTTCGGTCACTTCCAGGGTATGCAGGTCGATAAGACTGAGGCTGATGTCCTTATCGATGGAGACGCAGGCGGTCGAAGCGATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCACGATGTGCTCAAGCAG‑GGccc                                                                                                                        >  8:201413/1‑149 (MQ=255)
                         ggCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCg                                                                                                                       <  4:133041/149‑1 (MQ=255)
                            aGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTg                                                                                                                    <  2:38040/149‑1 (MQ=255)
                                    ggTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAggg                                                                                                            >  4:231821/1‑149 (MQ=255)
                                    ggTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAgg                                                                                                             <  4:207114/148‑1 (MQ=255)
                                        aCTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcg                                                                                                        >  6:232795/1‑149 (MQ=255)
                                          ttCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcggc                                                                                                      >  4:20604/1‑149 (MQ=255)
                                            cccgCGTTTGCAGGGCGATCAGGCGGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGGGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGggcggc                                                                                                      <  4:255548/144‑1 (MQ=255)
                                             cAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAag                                                                                                                <  5:235945/136‑1 (MQ=255)
                                             cAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAag                                                                                                                >  6:235945/1‑136 (MQ=255)
                                                      cAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGAtt                                                                                         >  7:213935/1‑149 (MQ=255)
                                                        ggTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTcc                                                                                       <  5:232795/149‑1 (MQ=255)
                                                         gTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCt                                                                                      <  2:184409/149‑1 (MQ=255)
                                                            gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                    <  6:151932/148‑1 (MQ=255)
                                                            gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                    <  3:181176/148‑1 (MQ=255)
                                                              tCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                    <  8:180240/146‑1 (MQ=255)
                                                              tCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTa                                                                                    >  7:180240/1‑146 (MQ=255)
                                                              tCAGGCTGAGGCCGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTAttg                                                                                 <  3:172212/149‑1 (MQ=255)
                                                                                    ctcGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAgggg                                                            <  3:231821/148‑1 (MQ=255)
                                                                                       gTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGAGTCCTTATTGTTGGGGTTGTCGGGTCAGGGGTAGc                                                        >  6:231028/1‑149 (MQ=255)
                                                                                                ccAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCT‑GGGTAAAGCAG‑TGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                                              >  2:99774/1‑149 (MQ=255)
                                                                                                ccAGGCGCTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGGATGG‑CGGAGGGCTTATTGTTGTGGTAGGCGGGGCAGGGGTAGCTACACGTCa                                               >  2:87232/1‑149 (MQ=255)
                                                                                                 cAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                                              >  5:35732/1‑149 (MQ=255)
                                                                                                      ggggggaGCCATGGTGTGTCTGCTTGCCAGGGCCAGGGCGGCCGACATCACGGTGGGGGAAAGGCG‑GGCCCGGTGGAAGATGGGGCGGCGGATGGTCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCg                                          <  1:205401/143‑1 (MQ=255)
                                                                                                             gCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTc                                  <  8:213935/149‑1 (MQ=255)
                                                                                                                  ccccagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑ATAGTTCCTATGAGGTGTGGGTGTGGGGTGAGGGGTAGCGACACGTCACATCGGGCGTGTGATAGc                             >  1:222141/8‑149 (MQ=12)
                                                                                                                     cagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑c                                                                                              <  2:23820/77‑1 (MQ=17)
                                                                                                                     cagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑c                                                                                              >  1:23820/5‑81 (MQ=17)
                                                                                                                        aCTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGCCGCTTGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAgg                        <  1:87232/149‑1 (MQ=255)
                                                                                                                                ccAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCAtt               <  5:139688/149‑1 (MQ=255)
                                                                                                                                 cAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCAtt               >  2:75592/1‑148 (MQ=255)
                                                                                                                                  aGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTc             >  2:3550/1‑149 (MQ=255)
                                                                                                                                     gcCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGtt          <  3:20604/149‑1 (MQ=255)
                                                                                                                                     gcCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGtt          >  7:57587/1‑149 (MQ=255)
                                                                                                                                         gggCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAg      <  6:69431/149‑1 (MQ=255)
                                                                                                                                             gcCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAGGGAt  <  8:57587/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAG‑GGCCCGGTGGAAGAGGGGGCGGCGCATGG‑CGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTCCATTTCGTTGAAGGGAT  >  NC_002947/3057153‑3057442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑