Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,129,240 G→A 9.7% E163K (GAA→AAA)  dmoA‑I → dimethyl‑sulfide monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,129,2400GA 9.7% 108.9 / 3.1 41E163K (GAA→AAA) dmoA‑Idimethyl‑sulfide monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base A (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

GTTCGCCCGGCGCCTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCA  >  NC_002947/3129095‑3129388
                                                                                                                                                 |                                                                                                                                                    
gTTCGCCCGGCGCCTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAAt                                                                                                                                                   <  4:34135/149‑1 (MQ=255)
      ccGGCGCCTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCtt                                                                                                                                              >  4:249956/1‑148 (MQ=255)
       cGGCGCCTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGACTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTg                                                                                                                                             >  3:130121/1‑148 (MQ=255)
           gcCTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTg                                                                                                                                        <  8:200420/149‑1 (MQ=255)
             cTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGct                                                                                                                                      <  3:249956/149‑1 (MQ=255)
                tCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGctct                                                                                                                                    <  2:25783/148‑1 (MQ=255)
                   aCCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTAc                                                                                                                                  >  1:120314/1‑147 (MQ=255)
                               cTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATa                                                                                                                                                  >  8:122628/1‑119 (MQ=255)
                               cTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATa                                                                                                                                                  <  7:122628/119‑1 (MQ=255)
                                          gcgcATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTggg                                                                                                            >  3:2465/1‑146 (MQ=255)
                                               tCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTTCTCTACAAGCTGTTCGAAggg                                                                                                                   <  5:266144/134‑1 (MQ=255)
                                               tCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAggg                                                                                                                   >  6:266144/1‑134 (MQ=255)
                                                      gAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAg                                                                                                                               <  3:223009/115‑1 (MQ=255)
                                                      gAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAg                                                                                                                               >  4:223009/1‑115 (MQ=255)
                                                           tCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCgg                                                                                        <  4:130121/149‑1 (MQ=255)
                                                           tCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCgg                                                                                        <  4:2465/149‑1 (MQ=255)
                                                              ttACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGAt                                                                                     >  1:256011/1‑149 (MQ=255)
                                                               tACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACAAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATc                                                                                    >  7:17950/1‑149 (MQ=255)
                                                               tACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACAAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATc                                                                                    >  7:21950/1‑149 (MQ=255)
                                                                   tcctACCTCGACAGCGGCGCGCGCAACCTCGAGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATcgc                                                                                  >  2:87364/1‑147 (MQ=255)
                                                                     ctACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAgc                                                                              >  3:176004/1‑149 (MQ=255)
                                                                     ctACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAgc                                                                              >  7:114786/1‑149 (MQ=255)
                                                                      tACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCCGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGCTCGCGAgcg                                                                             >  7:104745/1‑149 (MQ=255)
                                                                       aCCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAgcgc                                                                            >  4:241175/1‑149 (MQ=255)
                                                                       aCCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAgcgc                                                                            >  1:146644/1‑149 (MQ=255)
                                                                         cTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACAAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCgg                                                                          <  8:17950/149‑1 (MQ=255)
                                                                         cTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACAAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCgg                                                                          <  8:21950/149‑1 (MQ=255)
                                                                                           aaCCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTGGAAGGGAGCTGGGAAGAGGGAGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGaa                                                        >  2:259498/1‑149 (MQ=255)
                                                                                           aaCCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGaa                                                        <  2:256011/149‑1 (MQ=255)
                                                                                                       aaGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCAt                                              >  7:23377/1‑147 (MQ=255)
                                                                                                            gcTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATcc                                       >  1:262659/1‑149 (MQ=255)
                                                                                                                     ccACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAg                               >  2:140985/1‑148 (MQ=255)
                                                                                                                     ccACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAg                               <  1:140985/148‑1 (MQ=255)
                                                                                                                     ccACGATGCGCGCTACGACTACGCCGACGAATACCTTGAAGTTCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAgg                              <  8:104745/149‑1 (MQ=255)
                                                                                                                         gATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGTGATCGCGATCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCATGGCaa                          <  5:188497/149‑1 (MQ=255)
                                                                                                                         gATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGc                            >  6:191850/1‑147 (MQ=255)
                                                                                                                             cgcgcTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCa                       <  2:120314/148‑1 (MQ=255)
                                                                                                                                gcTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACttt                   <  3:241175/149‑1 (MQ=255)
                                                                                                                                    cGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAg                <  4:176004/148‑1 (MQ=255)
                                                                                                                                     gACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGt              <  8:114786/149‑1 (MQ=255)
                                                                                                                                                cGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGcatcc   <  5:191850/149‑1 (MQ=255)
                                                                                                                                                 gAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGcatcca  >  2:189161/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTTCGCCCGGCGCCTGTCGACCCTGGATCACCTGACCAAGGGGCGCATCGGCTGGAACATCGTTACCTCCTACCTCGACAGCGGCGCGCGCAACCTCGGGCAGAAGGCGCTGAGCGACCACGATGCGCGCTACGACTACGCGGACGAATACCTTGAAGTGCTCTACAAGCTGTTCGAAGGGAGCTGGGAAGAGGGCGCTGTGCTGCGGGATCGCGAGCGCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCA  >  NC_002947/3129095‑3129388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑