Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,173,382 C→T 10.8% T296I (ACC→ATC)  PP_2785 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,173,3820CT10.8% 94.8 / 3.1 37T296I (ACC→ATC) PP_2785hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/14);  new base T (2/2);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

CAGACCGAAGAGGGCCTGATTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCAT  >  NC_002947/3173246‑3173517
                                                                                                                                        |                                                                                                                                       
cAGACCGAAGAGGGCCTGATTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACGAATCCTTACcg                                                                                                                             >  4:1580/1‑149 (MQ=255)
       aaGAGGGCCTGATTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTAc                                                                                                                      >  3:20317/1‑149 (MQ=255)
             gCCTGATTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGc                                                                                                                <  7:72052/149‑1 (MQ=255)
              ccTGATTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGc                                                                                                                >  8:234136/1‑148 (MQ=255)
                  aTTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGAc                                                                                                             <  1:142209/147‑1 (MQ=255)
                    tCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGa                                                                                                         >  4:208379/1‑149 (MQ=255)
                    tCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGa                                                                                                         >  3:27719/1‑149 (MQ=255)
                        cTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGc                                                                                                     <  3:104789/149‑1 (MQ=255)
                               ggCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGAc                                                                                               >  6:29231/1‑148 (MQ=255)
                                 cAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCaa                                                                                            <  3:208379/149‑1 (MQ=255)
                                  aaTTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAg                                                                                           >  3:150837/1‑149 (MQ=255)
                                     tCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAACTCGCCTACTTCGACAATTGGCGCGTCACCCATCCTTACCGCGCTAACGGCAGCGACCAGACGGCTATGACCAAGTCg                                                                                        >  6:194729/1‑149 (MQ=255)
                                          cgcTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCgctgct                                                                                   >  3:110370/1‑149 (MQ=255)
                                            cTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTgg                                                                                 <  4:150837/149‑1 (MQ=255)
                                                   aTGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGctgctg                                                                                  >  7:141481/1‑141 (MQ=255)
                                                   aTGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGctgctg                                                                                  <  8:141481/141‑1 (MQ=255)
                                                       gCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTAc                                                                      <  3:92582/149‑1 (MQ=255)
                                                       gCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTAAGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTAc                                                                      >  6:273559/1‑149 (MQ=255)
                                                         ggggCCGGTGGGGCCATCGATGCCGGCCACAGCATCGTGGTCCGCAGTGCAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCACGTCGCTGCTGGGCGTCGATTACct                                                                    <  2:90719/146‑1 (MQ=255)
                                                         ggTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACct                                                                    <  4:110370/149‑1 (MQ=255)
                                                            gCCGGTGTGGCCATCGATGCCGGCCAGAGCTTCGTGGTACGCAGTGAAGTCGGCTACTTCGACTATTGGCGCGTCACCAATCCTTACCGCGCATACGGCAGCGACCAGACGGCTTGGACGAAGTCGCTGCTGGCCGTCGAGTAGCTCtt                                                                 >  6:179624/1‑149 (MQ=255)
                                                                       cATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGc                                                      >  5:27898/1‑149 (MQ=255)
                                                                            aTGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGAtc                                                 <  4:20317/149‑1 (MQ=255)
                                                                                 ggCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGAc                                            >  8:225183/1‑149 (MQ=255)
                                                                                  gCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACg                                           >  7:127075/1‑149 (MQ=255)
                                                                                                 tACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAg                            <  5:29231/149‑1 (MQ=255)
                                                                                                   cGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCATCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCg                          >  8:154712/1‑149 (MQ=255)
                                                                                                   cGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCATCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCg                          >  8:158859/1‑149 (MQ=255)
                                                                                                          aaGTCGCCTACTTCGACAATTGGCGCGTCACCAATACTTAGCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGgctgct                    <  5:194729/148‑1 (MQ=255)
                                                                                                               gCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACt               <  7:234136/148‑1 (MQ=255)
                                                                                                                 cTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTgg             >  2:119142/1‑148 (MQ=255)
                                                                                                                   aCTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAg          >  2:190445/1‑149 (MQ=255)
                                                                                                                     ttCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGcc        >  2:154650/1‑149 (MQ=255)
                                                                                                                        gACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCgg     >  4:120928/1‑149 (MQ=255)
                                                                                                                         aCAATTGGCGCGTCATCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGc    <  7:158859/149‑1 (MQ=255)
                                                                                                                         aCAATTGGCGCGTCATCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGc    <  7:154712/149‑1 (MQ=255)
                                                                                                                           aaTTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCAt  <  6:27898/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CAGACCGAAGAGGGCCTGATTCGCCTTGAGCGGCAATTCCCGCGCTATTCCATGGGCGGTGCCGGTGTGGCCATCGATGCCGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCAT  >  NC_002947/3173246‑3173517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑