Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 290,815 C→T 7.2% intergenic (+95/‑125) PP_0235 → / → ssuE peroxidase/NAD(P)H‑dependent FMN reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947290,8150CT 7.2% 157.2 / 2.3 55intergenic (+95/‑125)PP_0235/ssuEperoxidase/NAD(P)H‑dependent FMN reductase subunit
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

GCGGTCAAACCCTATCTGCGCCTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCTGGTCGTCTCAATCGG  >  NC_002947/290676‑290959
                                                                                                                                           |                                                                                                                                                
gCGGTCAAACCCTATCTGCGCCTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCagc                                                                                                                                          >  6:188256/1‑148 (MQ=255)
        aCCCTATCTGCGCCTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGcc                                                                                                                                 <  4:91574/149‑1 (MQ=255)
           cTATCTGCGCCTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTc                                                                                                                              >  7:5370/1‑149 (MQ=255)
             aTCTGCGCCTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCTTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGt                                                                                                                            <  7:93824/149‑1 (MQ=255)
                     cTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATa                                                                                                                       >  5:227704/1‑146 (MQ=255)
                        aCCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGAc                                                                                                                  <  5:106186/148‑1 (MQ=255)
                           ccTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGAt                                                                                                               >  5:230413/1‑148 (MQ=255)
                             tCAACCAAATAGTTAATGGATTCCTCGTTGCATTGCTCTTAGAAAAGCAGGGATTTTCGGGCCGTTACGACGGAACTTTTTTATGCCTGCAAAAAACCTATGGCACCGGGCGAGCCCCCGAACAGGCCCTCGGGTATAAAAAAGATaaa                                                                                                            >  4:253121/1‑148 (MQ=255)
                               aaCCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATg                                                                                                           <  6:227704/148‑1 (MQ=255)
                                 cccAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGaa                                                                                                        >  7:190097/1‑149 (MQ=255)
                                   cAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAAt                                                                                                       >  5:150333/1‑148 (MQ=255)
                                     aTCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATaaa                                                                                                    <  2:108449/149‑1 (MQ=255)
                                      tCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAAc                                                                                                   <  8:190097/149‑1 (MQ=255)
                                         ttAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGGCCTCGTGGATAAAGACGATAATGGAATagattcg                                                                                                >  3:73117/1‑143 (MQ=255)
                                         ttAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGaa                                                                                                        <  4:73117/141‑1 (MQ=255)
                                           aaTGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAAt                                                                                               <  6:150333/148‑1 (MQ=255)
                                                aTTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGaaaa                                                                                          >  1:182869/1‑148 (MQ=255)
                                                 ttCCTCGTTGATTTGCTTTTAGCCAAGCAGGTATTTTCTGGCGGTTTCGACGGCGTTTTTTTATTCCTGCAAAAAACCTGTGTTATCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGaaaaaa                                                                                        <  3:253121/149‑1 (MQ=255)
                                                     tCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTATTTTATGACTGCATAAAAGCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATTAAGACTATAATGGAATAATCAAATGAAAAAat                                                                                       >  8:121160/1‑146 (MQ=255)
                                                           cATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCt                                                                              <  6:230413/149‑1 (MQ=255)
                                                             ttGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTc                                                                               >  5:213336/1‑146 (MQ=255)
                                                                ctctTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGata                                                                         >  4:228498/1‑149 (MQ=255)
                                                                ctctTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGat                                                                          <  8:5370/148‑1 (MQ=255)
                                                                   ttAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGatat                                                                        <  3:228498/147‑1 (MQ=255)
                                                                     aGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCAGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATg                                                                     <  5:188256/148‑1 (MQ=255)
                                                                          aGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGa                                                                                             <  4:13059/119‑1 (MQ=255)
                                                                          aGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGa                                                                                             >  3:13059/1‑119 (MQ=255)
                                                                            cAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCt                                                             >  6:210853/1‑149 (MQ=255)
                                                                             aGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTg                                                            >  6:83366/1‑149 (MQ=255)
                                                                             aGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTg                                                            >  2:271033/1‑149 (MQ=255)
                                                                               ggATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGt                                                           <  1:271033/148‑1 (MQ=255)
                                                                                gATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGTGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGtt                                                          >  1:159074/1‑148 (MQ=255)
                                                                                gATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGTGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGtt                                                          >  3:31305/1‑148 (MQ=255)
                                                                                   tttCGGGCGGTTTCTACGGCCCTTTTTTATGCCTTCAAAAAACCTGTGGGAGCTGGCGCGCCCGCGAAGAGGCCCTCGTGTATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAAt                                                       <  1:241531/148‑1 (MQ=255)
                                                                                   tttCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTTGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAAt                                                       <  2:207268/148‑1 (MQ=255)
                                                                                   tttCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTGGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAAt                                                       >  1:207268/1‑148 (MQ=255)
                                                                                       gggCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGTGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCAc                                                  <  2:159074/149‑1 (MQ=255)
                                                                                       gggCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGTGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCAc                                                  <  4:31305/149‑1 (MQ=255)
                                                                                                 gACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTa                                                                             <  5:162053/112‑1 (MQ=255)
                                                                                                 gACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTa                                                                             >  6:162053/1‑112 (MQ=255)
                                                                                                       ccTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTc                                               >  6:14595/1‑136 (MQ=255)
                                                                                                       ccTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTc                                               <  5:14595/136‑1 (MQ=255)
                                                                                                       ccTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACaa                                  <  5:83366/149‑1 (MQ=255)
                                                                                                         ttttttATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTa                                                                             <  1:35178/104‑1 (MQ=255)
                                                                                                         ttttttATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTa                                                                             >  2:35178/1‑104 (MQ=255)
                                                                                                         ttttttATGCCTGCAAAAAACCTGT‑GGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCaa                                            <  1:101540/136‑1 (MQ=255)
                                                                                                         ttttttATGCCTGCAAAAAACCTGT‑GGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAAAGAAAAAATATGATTTATAGATATATGTAGCAGCTGTTAATGTAACATACaa                                            >  2:101540/1‑136 (MQ=255)
                                                                                                                   cTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCgcagc                       >  4:256827/1‑148 (MQ=255)
                                                                                                                        aaaaaCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCt                 >  3:21620/1‑149 (MQ=255)
                                                                                                                         aaaaCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATTGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCTg                <  4:21620/149‑1 (MQ=255)
                                                                                                                         aaaaCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCTg                <  6:213336/149‑1 (MQ=255)
                                                                                                                          aaaCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCACCAACAGAACACAAGGAAGCGCAGCAATGCTgg               <  5:210853/149‑1 (MQ=255)
                                                                                                                              cTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCTGgtcgt           >  6:249533/1‑149 (MQ=255)
                                                                                                                                     aGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGCCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCTGGTCGTCTCAATc    >  1:2729/1‑149 (MQ=255)
                                                                                                                                       cGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCCCCTCCAACAGAACACAAGGAAGCGCAGCAATGCTGGTCGTCTCAATCgg  <  1:144147/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GCGGTCAAACCCTATCTGCGCCTGACCCCTCAACCCAATCGTTAATGGATTCCTCGTTGCATTGCTCTTAGCCAAGCAGGGATTTTCGGGCCGTTTCGACGGCCCTTTTTTATGCCTGCAAAAAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCCTCGTGGATAAAGACGATAATGGAATAAACAAATGAAAAAATATGATTTCTAGATATATGTAGCAGCTGTTAATGTCACCTCCAACAGAACACAAGGAAGCGCAGCAATGCTGGTCGTCTCAATCGG  >  NC_002947/290676‑290959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑