| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,198,910 | A→T | 17.2% | intergenic (‑254/+23) | ethA ← / ← PP_2806 | FAD‑containing monooxygenase EthA/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,198,910 | 0 | A | T | 17.2% | 64.1 / 6.4 | 29 | intergenic (‑254/+23) | ethA/PP_2806 | FAD‑containing monooxygenase EthA/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (15/9); new base T (3/2); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAA > NC_002947/3198763‑3199040 | cttcTATTTCAGGATTATTTGCACCTGTTACTTCGCCGATCAACGCTCAGACTGGGTACGTCTGTTAGATATCGTCATCGGAACGCTCGACGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACgcag < 6:233/146‑1 (MQ=255) atTTGCACGTGTTACTTCGCCGTCCTTCCATCTGTCTGGGGCTGTCTGGGAGATCTCGGCCTCCGACCGTGCGACGGGGGGGGCTGCGGCGTTCCGCGCCGGTGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtgt < 8:45442/148‑1 (MQ=255) ttGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTggg > 7:141050/1‑149 (MQ=255) cTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCt > 3:63312/1‑147 (MQ=255) tGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAg > 7:18706/1‑149 (MQ=255) cGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGt > 3:9456/1‑142 (MQ=255) aGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑c > 6:151667/1‑149 (MQ=255) tCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTgg > 2:195813/1‑148 (MQ=255) cATCGGCACGTTCGACGGGGGGCGCGGCGGCCTGCCGCGCCGGCGGGGGCGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGc < 8:80501/149‑1 (MQ=255) cGTGCGACGGGGGGCGCGGCGGCTTTCTGAGCCGGAGGGGGCGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGc < 8:55079/149‑1 (MQ=255) gTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCg > 1:49807/1‑149 (MQ=255) ttcgtcggggggggGTGCGGCTTTCCGAGCGGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt < 4:63312/143‑1 (MQ=255) gAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGTGGTCTCGGTCTCGGCCTCCAGGCGTGCa > 2:87733/1‑149 (MQ=255) acggggggCGCGGCGGCGTTCCGAGCCGGAGGGGCAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCAc < 8:141050/147‑1 (MQ=255) aGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACg > 6:82982/1‑149 (MQ=255) gggCGCGGCGGCGTCCCCCGCCGGCCGGGGCGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACCTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTc < 1:195813/149‑1 (MQ=255) ctccGGCTCTGAAAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTGAGTAACTGGCCACCAGCAACTTGA‑CTGGCTGGCAGGCGGTCACGGTCTCGGCGACCAGGCGTGCACGGTCGCCGt > 6:42436/4‑148 (MQ=255) cGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCATGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAg > 8:218144/1‑148 (MQ=255) gggaggggaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAgg < 2:49807/149‑1 (MQ=255) ggggaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGt < 5:82982/149‑1 (MQ=255) ggggaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGt > 7:63579/1‑149 (MQ=255) gaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCg > 3:91818/1‑148 (MQ=255) attCGCCGTGCTGCGTCGCTGCACGGCG‑ATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGa > 2:226611/3‑133 (MQ=39) attCGCCGTGCTGCGTCGCTGCACGGCG‑ATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGa < 1:226611/131‑1 (MQ=39) aTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGaa < 5:151667/149‑1 (MQ=255) ccGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCa > 5:3512/1‑104 (MQ=255) ccGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCa < 6:3512/104‑1 (MQ=255) ccGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGaa > 1:134116/1‑145 (MQ=255) ccGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAgg > 4:68798/1‑75 (MQ=255) ccGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGaa > 2:225726/1‑145 (MQ=255) | CTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGA‑CTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAA > NC_002947/3198763‑3199040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |