Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,235,001 G→A 9.5% V32V (GTG→GTA) ‡ PP_2834 → D‑galactarate dehydratase/altronate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,235,0010GA 9.5% 116.4 / 2.8 42V32V (GTG→GTA) ‡PP_2834D‑galactarate dehydratase/altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base A (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

CAGTGGCTACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACG  >  NC_002947/3234855‑3235137
                                                                                                                                                  |                                                                                                                                        
cAGTGGCTACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTgcg                                                                                                                                        <  3:200750/149‑1 (MQ=255)
cAGTGGCTACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTgc                                                                                                                                         <  4:211567/148‑1 (MQ=255)
      cTACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGa                                                                                                                                  <  5:44254/149‑1 (MQ=255)
       tACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGCACGCCTggc                                                                                                                                  >  6:174640/1‑147 (MQ=255)
       tACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGCACGCCTggc                                                                                                                                  >  6:170709/1‑147 (MQ=255)
         cAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGCACGCCTggc                                                                                                                                  <  5:174640/146‑2 (MQ=255)
         cAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGCACGCCTggc                                                                                                                                  <  5:170709/146‑2 (MQ=255)
           gCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGa                                                                                                                                  <  6:275720/144‑1 (MQ=255)
           gCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGa                                                                                                                                  >  5:275720/1‑144 (MQ=255)
            ccATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGcc                                                                                                                              >  5:83882/1‑147 (MQ=255)
             cATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAg                                                                                                                           <  1:236858/149‑1 (MQ=255)
              aTAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAgg                                                                                                                          <  8:148185/149‑1 (MQ=255)
                 aCAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGa                                                                                                                       <  8:85061/149‑1 (MQ=255)
                           aGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTg                                                                                                                                    >  4:120398/1‑126 (MQ=255)
                           aGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTg                                                                                                                                    <  3:120398/126‑1 (MQ=255)
                              cGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCa                                                                                                          <  7:80754/149‑1 (MQ=255)
                                   cGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCa                                                                                                     <  2:217262/149‑1 (MQ=255)
                                              gTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGc                                                                                          >  1:64481/1‑149 (MQ=255)
                                                       aaCTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTc                                                                                 >  8:30665/1‑149 (MQ=255)
                                                       aaCTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTc                                                                                 >  2:46432/1‑149 (MQ=255)
                                                                ccAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAg                                                                        >  4:128147/1‑149 (MQ=255)
                                                                ccAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAg                                                                        >  4:231283/1‑149 (MQ=255)
                                                                 cAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTc                                                                                 >  3:67509/1‑139 (MQ=255)
                                                                 cAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTc                                                                                 <  4:67509/139‑1 (MQ=255)
                                                                  aaaTCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGcc                                                                      >  3:27356/1‑149 (MQ=255)
                                                                       cGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCtgtg                                                                  <  7:30665/148‑1 (MQ=255)
                                                                              cccGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAgg                                                          <  3:128147/149‑1 (MQ=255)
                                                                                     tCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCg                                                   >  8:133131/1‑149 (MQ=255)
                                                                                       aTCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGtt                                                 >  7:121115/1‑149 (MQ=255)
                                                                                            ccTCAACTCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGcc                                            <  6:83882/149‑1 (MQ=255)
                                                                                                              cgtcgtCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCg                          <  2:64481/149‑1 (MQ=255)
                                                                                                               gtcgtcATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCg                          >  3:202086/1‑148 (MQ=255)
                                                                                                                     aTCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGc                   <  7:133131/149‑1 (MQ=255)
                                                                                                                      tCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCg                  >  1:146229/1‑149 (MQ=255)
                                                                                                                         cccgccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTcc               >  7:127772/1‑149 (MQ=255)
                                                                                                                           cgccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGa                        <  4:92328/138‑1 (MQ=255)
                                                                                                                           cgccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGa                        >  3:92328/1‑138 (MQ=255)
                                                                                                                             ccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTgg                                                                 <  2:179963/95‑1 (MQ=255)
                                                                                                                             ccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTgg                                                                 >  1:179963/1‑95 (MQ=255)
                                                                                                                                ggCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGa        >  5:81582/1‑149 (MQ=255)
                                                                                                                                  cccTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATc      <  1:107972/149‑1 (MQ=255)
                                                                                                                                   ccTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATc      >  8:177936/1‑148 (MQ=255)
                                                                                                                                      gctgcCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACg  >  8:221759/1‑149 (MQ=255)
                                                                                                                                       ctgcCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGAc   <  8:46698/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
CAGTGGCTACAGCCATAACAAGAAGTCAGCCGCTCCGCCCTGAGAGGTCCCATGCAACTGATTGCCAAATCCGGTGACCCCGCCGTCATCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACG  >  NC_002947/3234855‑3235137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑