Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,236,450 G→A 10.2% intergenic (+3/‑57) PP_2834 → / → PP_2835 D‑galactarate dehydratase/altronate dehydratase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,236,4500GA10.2% 103.8 / 2.9 39intergenic (+3/‑57)PP_2834/PP_2835D‑galactarate dehydratase/altronate dehydratase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (17/18);  new base A (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

CTCCACCAGCATCGAGGAGCGCGGCGCATTCATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACGTCGCCCAGGTAGTGACCC  >  NC_002947/3236309‑3236585
                                                                                                                                             |                                                                                                                                       
cTCCACCAGCATCGTGGAGCGCGGCGCATTCATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTc                                                                                                                                  <  7:213084/149‑1 (MQ=255)
          aTCGAGGAGCGCGGCGCATTCATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCAtt                                                                                                                        <  8:125277/149‑1 (MQ=255)
                     cggcgCATTCATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAAc                                                                                                             <  5:221305/149‑1 (MQ=255)
                           aTTCATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCaa                                                                                                        >  6:53383/1‑148 (MQ=255)
                              cATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGAt                                                                                                    >  8:483/1‑149 (MQ=255)
                                  ttCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGAtt                                                                                                   >  3:212195/1‑146 (MQ=255)
                                  ttCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGAtt                                                                                                   <  4:212195/146‑1 (MQ=255)
                                    cGAGCAGATGCTGCGCATTGCCTCCTGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCa                                                                                                 <  4:83769/146‑1 (MQ=255)
                                     gAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGAAATGATTCAAAc                                                                                              >  2:271388/1‑148 (MQ=255)
                                          gATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACt                                                                                        <  5:53383/149‑1 (MQ=255)
                                          gATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCAACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACt                                                                                        >  2:125795/1‑149 (MQ=255)
                                                 cgcATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACa                                                                                   <  7:483/147‑1 (MQ=255)
                                                   cATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAAc                                                                                 >  2:145532/1‑147 (MQ=255)
                                                   cATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAAc                                                                                 <  1:145532/147‑1 (MQ=255)
                                                          tCCGGGGAACTCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCAGTCCCATTTGCCACGCAACTGCAATCATTAAAACCACACTGTACAACGTGCAAgcg                                                                        >  4:185877/1‑149 (MQ=255)
                                                                    gcagcaAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGt                                                              >  4:128617/1‑149 (MQ=255)
                                                                     cagcaAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGtt                                                             >  5:92224/1‑149 (MQ=255)
                                                                         aaGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTc                                                         <  1:271388/149‑1 (MQ=255)
                                                                           gagCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTc                                                         >  1:12435/1‑147 (MQ=255)
                                                                                      aCGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCa                                            >  5:246042/1‑149 (MQ=255)
                                                                                        ggCTACGGGAAAAACGAGTTCGTCCCTTGGAAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATg                                          <  3:185877/149‑1 (MQ=255)
                                                                                         gCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGa                                         <  3:128617/149‑1 (MQ=255)
                                                                                          cTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGa                                         >  7:201404/1‑148 (MQ=255)
                                                                                           tACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGa                                         >  3:114476/1‑147 (MQ=255)
                                                                                              gggCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGcc                                    >  6:120370/1‑149 (MQ=255)
                                                                                                gCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCgg                                  <  5:120370/149‑1 (MQ=255)
                                                                                                 cAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGAGCGACCGCTCCCGTCCCATTTGCGACGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGa                                 <  2:218901/149‑1 (MQ=255)
                                                                                                  aaaaCGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAg                                >  3:65155/1‑149 (MQ=255)
                                                                                                       gAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGAc                           <  6:92224/149‑1 (MQ=255)
                                                                                                        aGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCAACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTCCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACg                          >  6:99135/1‑149 (MQ=255)
                                                                                                             gTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCaa                                                   <  6:24/119‑1 (MQ=255)
                                                                                                             gTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCaa                                                   >  5:24/1‑119 (MQ=255)
                                                                                                             gTCCCGTGGCAGATCGGCGCCGTGACCTGATCAACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGAc                     <  1:125795/149‑1 (MQ=255)
                                                                                                                  gTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACTTCg                 <  7:242327/148‑1 (MQ=255)
                                                                                                                  gTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACGTCGc                <  8:201404/149‑1 (MQ=255)
                                                                                                                  gTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACCTCg                 >  8:242327/1‑148 (MQ=255)
                                                                                                                     gCAGATCGGCGCCGTGACCTGATCAACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTCCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACGTCGccc              <  5:99135/148‑1 (MQ=255)
                                                                                                                       aGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACGTCGCCCAgg           >  6:2368/1‑149 (MQ=255)
                                                                                                                                ccGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACGTCGCCCAGGTAGTGAccc  <  2:12435/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                       
CTCCACCAGCATCGAGGAGCGCGGCGCATTCATCTTCGAGCAGATGCTGCGCATTGCCTCCGGGGAACGCAGCAAGAGCGAACAGCACGGCTACGGGCAAAACGAGTTCGTCCCGTGGCAGATCGGCGCCGTGACCTGATCGACCGCTCCCGTCCCATTTGCGAGGCAACTGCAATGATTCAAACCACACTGTACAACGTGCAAGCGCTTACCACGTTTGTCGAGCAACTGTTCATGAAAGCCGGAGCCGACGTGGACGTCGCCCAGGTAGTGACCC  >  NC_002947/3236309‑3236585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑