Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,237,312 C→A 9.5% P269Q (CCG→CAG)  PP_2835 → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,237,3120CA 9.5% 123.7 / 4.5 42P269Q (CCG→CAG) PP_2835putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (20/18);  new base A (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

TGCCGCTCGGCGGCCAGGCCTTTGGCCACAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTCGACAGGCCTTGGAGCA  >  NC_002947/3237168‑3237459
                                                                                                                                                |                                                                                                                                                   
tGCCGCTCGGCGGCCAGGCCTTTGGCCACAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCgcgc                                                                                                                                                 >  8:5903/1‑149 (MQ=255)
           ggcCAGGCCTTTGGCCGCAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTcggcgg                                                                                                                                      <  7:218763/149‑1 (MQ=255)
                 gCCTTTGGCCACAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTg                                                                                                                                  >  4:140457/1‑147 (MQ=255)
                      tGGCCACAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCg                                                                                                                            <  4:116145/148‑1 (MQ=255)
                        gCCACAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTc                                                                                                                         >  7:167794/1‑149 (MQ=255)
                           acaAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTc                                                                                                                      >  1:93912/1‑149 (MQ=255)
                             aaaGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTcga                                                                                                                    <  7:45609/149‑1 (MQ=255)
                               aGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTcgac                                                                                                                   >  2:8872/1‑148 (MQ=255)
                                 gTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCa                                                                                                                                                <  4:205497/117‑1 (MQ=255)
                                 gTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCa                                                                                                                                                >  3:205497/1‑117 (MQ=255)
                                      gCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCa                                                                                                            <  7:104431/148‑1 (MQ=255)
                                      gCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAg                                                                                                           >  4:88114/1‑149 (MQ=255)
                                                  ttGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTggggga                                                                                               >  2:59676/1‑148 (MQ=255)
                                                       cGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTgg                                                                                          <  2:93912/149‑1 (MQ=255)
                                                        gAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGaaa                                                                                                               <  2:262461/127‑1 (MQ=255)
                                                        gAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGaaa                                                                                                               >  1:262461/1‑127 (MQ=255)
                                                          aGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTc                                                                                       <  4:192278/149‑1 (MQ=255)
                                                                 aCCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCaa                                                                                 <  3:140457/148‑1 (MQ=255)
                                                                   cTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGc                                                                                                                             >  1:204454/1‑102 (MQ=255)
                                                                   cTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGc                                                                                                                             <  2:204454/102‑1 (MQ=255)
                                                                        ggCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCT‑GGGGGATTGGTTCTGGCAAGccgcccgc                                                                        <  1:59676/149‑1 (MQ=255)
                                                                                  ccgTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCg                                                               <  5:34250/149‑1 (MQ=255)
                                                                                     tGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAAc                                                            <  8:167794/149‑1 (MQ=255)
                                                                                        gcgcAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTCCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGcc                                                         >  5:177256/1‑149 (MQ=255)
                                                                                          gcAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCg                                                       >  7:155290/1‑149 (MQ=255)
                                                                                            aaGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGt                                                     <  3:9800/149‑1 (MQ=255)
                                                                                                aTGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGc                                                 >  4:229655/1‑149 (MQ=255)
                                                                                                         aTCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCggg                                        <  3:229655/149‑1 (MQ=255)
                                                                                                               ggggCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCGTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGcc                                  >  4:207587/1‑148 (MQ=255)
                                                                                                                gggCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCa                                  <  1:8872/148‑1 (MQ=255)
                                                                                                                       tCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGc                          >  2:202481/1‑149 (MQ=255)
                                                                                                                       tCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGc                          >  5:244321/1‑149 (MQ=255)
                                                                                                                       tCGACCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGTGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGc                          >  8:87368/1‑149 (MQ=255)
                                                                                                                             catGCGCGGGTCAATCACCAGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCgg                                         >  3:3584/3‑128 (MQ=255)
                                                                                                                             catGCGCGGGTCAATCACCAGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCgg                                         >  1:175673/3‑128 (MQ=255)
                                                                                                                             catGCGCGGGTCAATCACCAGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCgg                                         <  2:175673/126‑1 (MQ=255)
                                                                                                                             catGCGCGGGTCAATCACCAGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCgg                                         <  4:3584/126‑1 (MQ=255)
                                                                                                                               tGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTc                  >  2:190412/1‑149 (MQ=255)
                                                                                                                                 cgcTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGTGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTCGa                <  7:87368/149‑1 (MQ=255)
                                                                                                                                    tGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGc                          <  6:89957/136‑1 (MQ=255)
                                                                                                                                    tGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGc                          >  5:89957/1‑136 (MQ=255)
                                                                                                                                             aCCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTCGACAGGCCTTGGAg    >  8:134935/1‑149 (MQ=255)
                                                                                                                                              cccGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGTAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTCGACAGGCCTTGGAg    >  2:212447/1‑148 (MQ=255)
                                                                                                                                               ccGCGCATGTGCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTCGACAGGCCTTGGAGCa  <  8:250491/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGCCGCTCGGCGGCCAGGCCTTTGGCCACAAAGGTTTTGCCCTTGCGCTGTTGGTCGAAGCCCTCACCTCGGGGCTGGCCGGCCGTGGGCGCAAGGATGCACCGGATCAGTGGGGCGCCTCGGCCACTGCGCTGGTGATTGACCCGCGCATGTTCGGCGGCCTGGAGGCGTTCCTCGACGAAACCAGCTTCCTGGGGGGATTGGTTCTGGCAAGCCGCCCGCTAGACCCCGAACGCCCGGTACGCCTGCCCGGGCAAGCAGGCCTTGCCCTGCGTCGACAGGCCTTGGAGCA  >  NC_002947/3237168‑3237459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑