Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 305,154 G→C 7.5% intergenic (+12/+17) hslR → / ← PP_0251 heat shock protein Hsp15/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947305,1540GC 7.5% 164.1 / 2.4 52intergenic (+12/+17)hslR/PP_0251heat shock protein Hsp15/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (22/26);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACCGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGC  >  NC_002947/305006‑305299
                                                                                                                                                    |                                                                                                                                                 
aaaCCGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGctgctg                                                                                                                                                   <  4:38200/149‑1 (MQ=255)
    cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTgcg                                                                                                                                                 >  4:20527/1‑147 (MQ=255)
               tgCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCattcaa                                                                                                                                    <  7:125956/149‑6 (MQ=255)
                 cGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATc                                                                                                                                  >  3:52998/1‑149 (MQ=255)
                   tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATc                                                                                                                                  >  5:170570/1‑147 (MQ=255)
                   tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTa                                                                                                                                <  8:42158/149‑1 (MQ=255)
                   tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTa                                                                                                                                <  2:246883/149‑1 (MQ=255)
                   tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCattcaatc                                                                                                                                  <  5:251953/147‑8 (MQ=255)
                   tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCattcaatc                                                                                                                                  >  6:251953/1‑140 (MQ=255)
                    cGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAg                                                                                                                               <  5:222951/149‑1 (MQ=255)
                           aaCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAaca                                                                                                                        >  4:116618/1‑149 (MQ=255)
                           aaCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAaca                                                                                                                        <  4:138057/149‑1 (MQ=255)
                                    cTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTc                                                                                                               <  3:57338/149‑1 (MQ=255)
                                       aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTc                                                                                                            <  5:86595/149‑1 (MQ=255)
                                       aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTc                                                                                                            <  5:262362/149‑1 (MQ=255)
                                         aTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc                                                                                                           >  6:111395/1‑148 (MQ=255)
                                            cGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGAc                                                                                                        <  3:20527/148‑1 (MQ=255)
                                                aGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACg                                                                                                   <  4:248644/149‑1 (MQ=255)
                                                 ggCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCTACACCGCCATTCGTCCGACCAACgg                                                                                                  >  5:209143/1‑149 (MQ=255)
                                                 ggCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACgg                                                                                                  <  2:205089/149‑1 (MQ=255)
                                                  gCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGt                                                                                                 <  5:222298/149‑1 (MQ=255)
                                                    cGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTaa                                                                                               >  4:44218/1‑149 (MQ=255)
                                                     gtggCCATGGTCGGTCCCACCGATGGCCGGCCGACCACGCAGCAGCGGCGGCACATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAAc                                                                                              <  7:233730/147‑1 (MQ=255)
                                                             gggCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAcag                                                                                      >  7:234993/1‑149 (MQ=255)
                                                                     ccaccGATGGCCGGCCGACCAAGAAGCAGCGGCGCCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGa                                                                              <  7:152010/149‑1 (MQ=255)
                                                                      caccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa                                                                             >  1:129268/1‑149 (MQ=255)
                                                                       accGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGAGCAGCCCCAAAAAATCTAGCTCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaac                                                                            <  6:46445/149‑1 (MQ=255)
                                                                           aTGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACg                                                                        >  1:126470/1‑149 (MQ=255)
                                                                                cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAca                                                                                       <  6:110978/129‑1 (MQ=255)
                                                                                cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAca                                                                                       >  5:110978/1‑129 (MQ=255)
                                                                                     gACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTc                                                                >  1:173621/1‑147 (MQ=255)
                                                                                      aCCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAg                                                             >  4:86274/1‑149 (MQ=255)
                                                                                       ccAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAGAAACGGCGCTGTCCAgc                                                            >  8:232677/1‑149 (MQ=255)
                                                                                       ccAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgc                                                            <  3:44218/149‑1 (MQ=255)
                                                                                        cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcg                                                           >  4:203158/1‑149 (MQ=255)
                                                                                          agaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCa                                                         >  5:224843/1‑149 (MQ=255)
                                                                                           gaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCAt                                                        <  3:203158/149‑1 (MQ=255)
                                                                                              gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCaa                                                     <  3:116618/149‑1 (MQ=255)
                                                                                              gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCaa                                                     >  6:251706/1‑149 (MQ=255)
                                                                                               cagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc                                                    <  7:225784/149‑1 (MQ=255)
                                                                                                  cggcggCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGcc                                                 >  1:47837/1‑149 (MQ=255)
                                                                                                            ttCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCtt                                       <  2:173621/149‑1 (MQ=255)
                                                                                                                ccAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgc                                   >  2:162851/1‑149 (MQ=255)
                                                                                                                     tGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc                                >  8:43355/1‑147 (MQ=255)
                                                                                                                           gAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcggc                         <  5:111395/148‑1 (MQ=255)
                                                                                                                           gAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcggc                         <  3:86274/148‑1 (MQ=255)
                                                                                                                             aGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCATCACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCTCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgt                       <  6:170570/148‑1 (MQ=255)
                                                                                                                                        aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGc                                                  <  1:82502/110‑1 (MQ=255)
                                                                                                                                        aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGc                                                  >  2:82502/1‑110 (MQ=255)
                                                                                                                                        aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc                                >  7:86071/1‑128 (MQ=255)
                                                                                                                                        aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc                                <  8:86071/128‑1 (MQ=255)
                                                                                                                                           ggggCTGCGCAGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccagcc        >  2:151201/1‑149 (MQ=255)
                                                                                                                                             ggCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagt                  <  2:162212/137‑1 (MQ=255)
                                                                                                                                             ggCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagt                  >  1:162212/1‑137 (MQ=255)
                                                                                                                                                tgctgcGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcg   >  1:61712/1‑149 (MQ=255)
                                                                                                                                                  ctgcGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcgc  <  6:224843/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AAACCGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGC  >  NC_002947/305006‑305299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑