Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,266,116 G→C 10.9% intergenic (+33/+26) PP_2863 → / ← PP_2864 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,266,1160GC10.9% 127.9 / 7.9 46intergenic (+33/+26)PP_2863/PP_2864membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/18);  new base C (2/3);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGAC  >  NC_002947/3265978‑3266261
                                                                                                                                            |                                                                                                                                                 
ccGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagag                                                                                                                                         <  5:215029/149‑4 (MQ=38)
ccGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagag                                                                                                                                         >  6:215029/1‑146 (MQ=38)
  gCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagaggc                                                                                                                                       <  3:13874/149‑6 (MQ=38)
     aGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCgg                                                                                                                                    <  5:241236/149‑1 (MQ=255)
       gTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                                  <  7:186843/149‑1 (MQ=255)
        tttCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                                  >  5:115203/1‑148 (MQ=255)
             ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                            >  5:133874/1‑149 (MQ=255)
             ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                            >  4:32064/1‑149 (MQ=255)
             ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                            >  6:30557/1‑149 (MQ=255)
                      gtgtGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTCCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATg                                                                                                                   <  1:85437/149‑1 (MQ=255)
                                   ttGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGTCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCt                                                                                                      <  4:128180/149‑1 (MQ=255)
                                      tgctgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCGTTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCAGGAACAGGTCGATCACATGACCAACCCCACCTg                                                                                                     >  1:77264/1‑149 (MQ=255)
                                         tgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCaa                                                                                                  <  1:214610/147‑1 (MQ=255)
                                         tgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCaa                                                                                                  >  2:214610/1‑147 (MQ=255)
                                           ctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCg                                                                                               <  3:32064/148‑1 (MQ=255)
                                           ctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCg                                                                                               <  5:30557/148‑1 (MQ=255)
                                               aCCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCtt                                                                                            >  3:172137/1‑147 (MQ=255)
                                               aCCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCtt                                                                                            <  4:172137/147‑1 (MQ=255)
                                                    gtgtTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAAc                                                                                     >  6:275821/1‑149 (MQ=255)
                                                    gtgtTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAAc                                                                                     >  6:138532/1‑149 (MQ=255)
                                                       ttCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGa                                                                                  >  6:204627/1‑149 (MQ=255)
                                                             gCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCAc                                                                             >  1:248024/1‑148 (MQ=255)
                                                                 aCTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCa                                                                        <  6:133874/149‑1 (MQ=255)
                                                                   tGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAg                                                                       >  4:204654/1‑148 (MQ=255)
                                                                     gCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAg                                                                       <  3:204654/146‑1 (MQ=255)
                                                                     gCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGc                                                                      <  2:77264/147‑1 (MQ=255)
                                                                      cTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTgg                                                                   >  1:117079/1‑149 (MQ=255)
                                                                                     cGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAac                                                    >  4:4925/1‑149 (MQ=255)
                                                                                            tGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTca                                              >  4:164774/1‑148 (MQ=255)
                                                                                             gCCCC‑‑TTGCTTGAAGTGCAGTTGTACCGGCCTCTACGCCCGTAAAGCCGCGAAAGCCCCGCAACACGTCGATCACAAGACCAACCCCACCACCCACCCCATCGGCAACCGACGCACTACCACCAGCACCGACCGCTCCAACACCTcaca                                            >  6:115186/1‑149 (MQ=255)
                                                                                                 c‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGt                                        >  4:60229/1‑149 (MQ=255)
                                                                                                    ttGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGc                                                                                              <  7:96352/94‑1 (MQ=255)
                                                                                                    ttGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGc                                                                                              >  8:96352/1‑94 (MQ=255)
                                                                                                        ttGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtcatc                                   <  5:204627/149‑1 (MQ=255)
                                                                                                              tgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGccc                             >  8:105668/1‑149 (MQ=255)
                                                                                                                  gTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCg                                                           <  7:213075/115‑1 (MQ=255)
                                                                                                                  gTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCg                                                           >  8:213075/1‑115 (MQ=255)
                                                                                                                   ttGTGCCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATgg                        >  8:161594/1‑149 (MQ=255)
                                                                                                                   ctatctcGGCCCTTTCGCGGCTTTACCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATgg                        <  3:243763/143‑1 (MQ=255)
                                                                                                                     gTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCAGACAGTTCATCACGCCCCATggg                       <  5:115186/148‑1 (MQ=255)
                                                                                                                        ccGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTa                    <  2:117079/148‑1 (MQ=255)
                                                                                                                         cGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAgg                  >  3:159482/1‑149 (MQ=255)
                                                                                                                           gccctttCGCGGCTTTACCCGCGAAGAGGCCGGTACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGCCGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGggc                >  7:183744/6‑149 (MQ=38)
                                                                                                                             ctctTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGggcgg              >  3:226853/1‑149 (MQ=255)
                                                                                                                                    cgGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATg       <  5:138532/149‑1 (MQ=255)
                                                                                                                                          aaGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGAc  <  3:164774/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
CCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCC‑‑TTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGAC  >  NC_002947/3265978‑3266261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑