Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,280,328 T→G 9.8% E101A (GAA→GCA)  PP_2880 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,280,3280TG 9.8% 107.8 / 4.2 41E101A (GAA→GCA) PP_2880TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base G (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GATCAGCACCCAGGCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTACCACCTCGGTAAACAGGTCGTCCTTG  >  NC_002947/3280183‑3280471
                                                                                                                                                 |                                                                                                                                               
gATCAGCACCCAGGCCAGCTGCTCATACTCCAGGTTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACAGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCgg                                                                                                                                              >  4:68212/1‑149 (MQ=255)
 aTCAGCACCCAGGCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCg                                                                                                                                               <  8:182604/147‑1 (MQ=255)
    aGCACCCAGGCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTGCgcaatg                                                                                                                                          >  8:40088/1‑144 (MQ=255)
       aCCCAGGCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACACAGCGGCGGCAACGGGCATCCACCTTCGGCATTgcg                                                                                                                                       >  3:149722/1‑149 (MQ=255)
            ggCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGt                                                                                                                                  <  5:244115/149‑1 (MQ=255)
             gCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTg                                                                                                                                 <  5:182497/149‑1 (MQ=255)
                 gctgctCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTGCGCAATGCCGAAGGTGGatg                                                                                                                             <  7:40088/149‑3 (MQ=255)
                   tgctCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTGCGCAATGCCGAAGGTGGatg                                                                                                                             <  6:67033/147‑3 (MQ=255)
                   tgctCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTGCGCAATGCCGAAGGTGGatg                                                                                                                             >  5:67033/1‑145 (MQ=255)
                     ctCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGc                                                                                                                         >  5:149972/1‑149 (MQ=255)
                         tcctccTGGGTTGGCCTCCTGGCCCGGGGCAGTTCAACAAGCCAGTGCTAAGCCATTTCTCGCACCGGCGTGTCGGTCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAg                                                                                                                     <  1:84374/149‑1 (MQ=255)
                            tccAGGGTTGAGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACAGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTa                                                                                                                  >  2:267343/1‑149 (MQ=255)
                                          ccAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCAAACCCAGCGGCGGAAACGGGCATCCACCTTCGGAATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGc                                                                                                    >  7:184650/1‑149 (MQ=255)
                                                      cAGTTCTACCAGCAAGTGCTCAGGCACTTCACGCACCGGCGTGTCGGCCGGTGCGATTTCGACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGtt                                                                                        <  8:184650/149‑1 (MQ=255)
                                                              ccagccagTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGtt                                                                                 <  2:163715/148‑1 (MQ=255)
                                                               cagccagTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACtgctgc                                                                                                           >  3:83409/1‑121 (MQ=255)
                                                               cagccagTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACtgctgc                                                                                                           <  4:83409/121‑1 (MQ=255)
                                                                  ccagTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACtgctgc                                                                                                           >  7:190167/1‑118 (MQ=255)
                                                                  ccagTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACtgctgc                                                                                                           <  8:190167/118‑1 (MQ=255)
                                                                         tCAGCCACTTCACGCACCGGCGTGTCGGCCCGCGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCt                                                                     >  7:164059/1‑149 (MQ=255)
                                                                           aGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCGACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTg                                                                    <  8:82147/148‑1 (MQ=255)
                                                                             ccACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGtt                                                                                        >  4:88817/1‑126 (MQ=255)
                                                                             ccACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGtt                                                                                        <  3:88817/126‑1 (MQ=255)
                                                                                   cACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGt                                                           >  8:210147/1‑149 (MQ=255)
                                                                                     cGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTTGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGc                                                         <  4:149722/149‑1 (MQ=255)
                                                                                          cGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAggg                                                    <  4:237559/149‑1 (MQ=255)
                                                                                          cGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAggg                                                    >  3:237559/1‑149 (MQ=255)
                                                                                            gCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAggggg                                                  <  3:68212/149‑1 (MQ=255)
                                                                                               tgtCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTg                                               <  6:149972/149‑1 (MQ=255)
                                                                                                 tCGGCCCGGGCGATTTACACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGAGTGCCAGGGGGCTGtt                                             >  2:119405/1‑149 (MQ=255)
                                                                                                                 ccACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCg                              >  6:144462/1‑148 (MQ=255)
                                                                                                                   acagCCAGCGGCGGCAACGGGCATGCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTac                           <  5:40840/149‑1 (MQ=255)
                                                                                                                   acacCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTac                           <  5:144462/149‑1 (MQ=255)
                                                                                                                       ccAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTaccacc                       >  6:144283/1‑149 (MQ=255)
                                                                                                                       ccAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTaccac                        >  4:107044/1‑148 (MQ=255)
                                                                                                                         aGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTACCACCTc                     <  7:210147/149‑1 (MQ=255)
                                                                                                                             gcggcAACGGGCATCCACCTACGGCATTGCGCAGGTGCACCAGGCTCAGGTACTGCTGCAGGCAGAGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTACCACCTCGGt                  >  6:98861/1‑148 (MQ=255)
                                                                                                                                  aaCGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTACCACCTCGGTAAACAg            >  2:210240/1‑149 (MQ=255)
                                                                                                                                            cACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTACCACCTCGGTAAACAGGTCGTCCTTg  <  8:164059/149‑1 (MQ=255)
                                                                                                                                             aCCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAg                                                                                             <  6:270184/57‑1 (MQ=255)
                                                                                                                                             aCCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAg                                                                                             >  5:270184/1‑57 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GATCAGCACCCAGGCCAGCTGCTCATCCTCCAGGGTTGTGCTCCAGGCCCGGTGCAGTTCTACCAGCCAGTGCTCAGCCACTTCACGCACCGGCGTGTCGGCCCGGGCGATTTCCACACCCAGCGGCGGCAACGGGCATCCACCTTCGGCATTGCGCAGGTGGACCAGGCTCAGGTACTGCTGCAGGCAGCGGCCCAGGCGTTCACGGTTGGCTCCCTTGCTGGCCAGGCGTGCCAGGGGGCTGTTGCTCAGCTCCTGGCGTACCACCTCGGTAAACAGGTCGTCCTTG  >  NC_002947/3280183‑3280471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑