Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 305,155 C→A 9.4% intergenic (+13/+16) hslR → / ← PP_0251 heat shock protein Hsp15/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947305,1550CA 9.4% 160.3 / 4.5 52intergenic (+13/+16)hslR/PP_0251heat shock protein Hsp15/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base A (2/3);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGCCAG  >  NC_002947/305010‑305302
                                                                                                                                                 |                                                                                                                                                   
cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTgcg                                                                                                                                                    >  4:20527/1‑147 (MQ=255)
           tgCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCattcaa                                                                                                                                       <  7:125956/149‑6 (MQ=255)
             cGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATc                                                                                                                                     >  3:52998/1‑149 (MQ=255)
               tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATc                                                                                                                                     >  5:170570/1‑147 (MQ=255)
               tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTa                                                                                                                                   <  8:42158/149‑1 (MQ=255)
               tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTa                                                                                                                                   <  2:246883/149‑1 (MQ=255)
               tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCattcaatc                                                                                                                                     >  6:251953/1‑140 (MQ=255)
               tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCattcaatc                                                                                                                                     <  5:251953/147‑8 (MQ=255)
                cGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAg                                                                                                                                  <  5:222951/149‑1 (MQ=255)
                       aaCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAaca                                                                                                                           <  4:138057/149‑1 (MQ=255)
                       aaCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAaca                                                                                                                           >  4:116618/1‑149 (MQ=255)
                                cTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTc                                                                                                                  <  3:57338/149‑1 (MQ=255)
                                   aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTc                                                                                                               <  5:86595/149‑1 (MQ=255)
                                   aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTc                                                                                                               <  5:262362/149‑1 (MQ=255)
                                     aTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc                                                                                                              >  6:111395/1‑148 (MQ=255)
                                        cGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGAc                                                                                                           <  3:20527/148‑1 (MQ=255)
                                            aGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACg                                                                                                      <  4:248644/149‑1 (MQ=255)
                                             ggCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCTACACCGCCATTCGTCCGACCAACgg                                                                                                     >  5:209143/1‑149 (MQ=255)
                                             ggCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACgg                                                                                                     <  2:205089/149‑1 (MQ=255)
                                              gCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGt                                                                                                    <  5:222298/149‑1 (MQ=255)
                                                cGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTaa                                                                                                  >  4:44218/1‑149 (MQ=255)
                                                 gtggCCATGGTCGGTCCCACCGATGGCCGGCCGACCACGCAGCAGCGGCGGCACATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAAc                                                                                                 <  7:233730/147‑1 (MQ=255)
                                                         gggCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAcag                                                                                         >  7:234993/1‑149 (MQ=255)
                                                                 ccaccGATGGCCGGCCGACCAAGAAGCAGCGGCGCCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGa                                                                                 <  7:152010/149‑1 (MQ=255)
                                                                  caccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa                                                                                >  1:129268/1‑149 (MQ=255)
                                                                   accGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGAGCAGCCCCAAAAAATCTAGCTCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaac                                                                               <  6:46445/149‑1 (MQ=255)
                                                                       aTGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACg                                                                           >  1:126470/1‑149 (MQ=255)
                                                                            cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAca                                                                                          <  6:110978/129‑1 (MQ=255)
                                                                            cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAca                                                                                          >  5:110978/1‑129 (MQ=255)
                                                                                 gACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTc                                                                   >  1:173621/1‑147 (MQ=255)
                                                                                  aCCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAg                                                                >  4:86274/1‑149 (MQ=255)
                                                                                   ccAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAGAAACGGCGCTGTCCAgc                                                               >  8:232677/1‑149 (MQ=255)
                                                                                   ccAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgc                                                               <  3:44218/149‑1 (MQ=255)
                                                                                    cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcg                                                              >  4:203158/1‑149 (MQ=255)
                                                                                      agaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCa                                                            >  5:224843/1‑149 (MQ=255)
                                                                                       gaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCAt                                                           <  3:203158/149‑1 (MQ=255)
                                                                                          gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCaa                                                        <  3:116618/149‑1 (MQ=255)
                                                                                          gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCaa                                                        >  6:251706/1‑149 (MQ=255)
                                                                                           cagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc                                                       <  7:225784/149‑1 (MQ=255)
                                                                                              cggcggCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGcc                                                    >  1:47837/1‑149 (MQ=255)
                                                                                                        ttCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCtt                                          <  2:173621/149‑1 (MQ=255)
                                                                                                            ccAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgc                                      >  2:162851/1‑149 (MQ=255)
                                                                                                                 tGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc                                   >  8:43355/1‑147 (MQ=255)
                                                                                                                       gAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcggc                            <  5:111395/148‑1 (MQ=255)
                                                                                                                       gAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcggc                            <  3:86274/148‑1 (MQ=255)
                                                                                                                         aGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCATCACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCTCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgt                          <  6:170570/148‑1 (MQ=255)
                                                                                                                                    aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGc                                                     <  1:82502/110‑1 (MQ=255)
                                                                                                                                    aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGc                                                     >  2:82502/1‑110 (MQ=255)
                                                                                                                                    aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc                                   >  7:86071/1‑128 (MQ=255)
                                                                                                                                    aaTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc                                   <  8:86071/128‑1 (MQ=255)
                                                                                                                                       ggggCTGCGCAGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccagcc           >  2:151201/1‑149 (MQ=255)
                                                                                                                                         ggCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagt                     <  2:162212/137‑1 (MQ=255)
                                                                                                                                         ggCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagt                     >  1:162212/1‑137 (MQ=255)
                                                                                                                                            tgctgcGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcg      >  1:61712/1‑149 (MQ=255)
                                                                                                                                              ctgcGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcgc     <  6:224843/148‑1 (MQ=255)
                                                                                                                                                 cgcAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGCCAg  >  5:167728/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGCCAG  >  NC_002947/305010‑305302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑