Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,304,764 C→G 11.6% intergenic (+17/+15) cysS → / ← PP_2906 cysteine‑‑tRNA ligase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,304,7640CG11.6% 115.2 / 7.0 43intergenic (+17/+15)cysS/PP_2906cysteine‑‑tRNA ligase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (21/17);  new base G (2/3);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

CCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGCCG  >  NC_002947/3304618‑3304911
                                                                                                                                                  |                                                                                                                                                   
ccAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGAGTACCTGGCGTCTGGCTGAATGAAGTAACTGGGGCTGCACCg                                                                                                                                                   >  5:131233/1‑149 (MQ=255)
 cgcacgcgcCTTCAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCg                                                                                                                                                   >  4:103437/5‑148 (MQ=255)
  gcacgcgcCTTCAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCa                                                                                                                                                 <  3:103437/146‑1 (MQ=255)
         ccTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCAg                                                                                                                                          <  4:137793/149‑1 (MQ=255)
          cTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCAcc                                                                                                                                                    <  8:21986/138‑1 (MQ=255)
          cTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCAcc                                                                                                                                                    >  7:21986/1‑138 (MQ=255)
              aGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCtttt                                                                                                                                     <  7:66812/149‑1 (MQ=255)
                            aaGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                           >  4:237955/1‑129 (MQ=255)
                            aaGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                           <  3:237955/129‑1 (MQ=255)
                            aaGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGAccc                                                                                                                       >  2:37568/1‑149 (MQ=255)
                            aaGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGAccc                                                                                                                       <  4:134644/149‑1 (MQ=255)
                            aaGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGAccc                                                                                                                       <  2:74819/149‑1 (MQ=255)
                                 cTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGCCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAg                                                                                                                  >  5:13315/1‑149 (MQ=255)
                                           tCTTACCCCATTCGCGATAAGCTGACCCCCAAGGGTGTTGTGGGGGAGGGCAGCAAGGGGACGACAACCTGGCGTGTGGCTGACTGAAGTAACTGGGGCTGCACCGCCGCCCCAGCGTTTTTTTTCTTATACCCCCAACCGGGCAggg                                                                                                         >  2:211266/1‑146 (MQ=255)
                                              gACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                           <  2:252631/111‑1 (MQ=255)
                                              gACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                           >  1:252631/1‑111 (MQ=255)
                                                        gcgATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTgg                                                                                           >  7:169901/1‑149 (MQ=255)
                                                         cgATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGc                                                                                          <  1:37568/149‑1 (MQ=255)
                                                              aGCTGACCCCCATGGGTGTAGTGCTGGAGGACAGCAAGCGCACGACTACCTGGCGTCTGGATGACTGAAGCAACTGCGGCTGCACCGCAGCACCAGCTTATAATTGCTTAGACCCGCAAGCGCCCACTGCAATACCAGCCTGCCcccac                                                                                     >  1:128883/1‑149 (MQ=255)
                                                                         aTGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCtt                                                                          <  1:211266/149‑1 (MQ=255)
                                                                            ggTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTcc                                                                        >  8:73621/1‑148 (MQ=255)
                                                                            ggTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTcc                                                                        <  7:73621/148‑1 (MQ=255)
                                                                              tgtAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTcccc                                                                      >  1:243236/1‑148 (MQ=255)
                                                                                tAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCtt                                                                          <  2:37970/142‑1 (MQ=255)
                                                                                tAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCtt                                                                          >  1:37970/1‑142 (MQ=255)
                                                                                     cTGGAGGACTGCAAGGGCACGTCTTCCTGGCGTCTGGCTGACTGAAGCAACTGGGGCTGCACCGCAGCGCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTGGCTTCCACCAAtctc                                                               <  2:128883/148‑1 (MQ=255)
                                                                                            aCAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCg                                                        >  8:67046/1‑148 (MQ=255)
                                                                                             cAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGa                                                       >  3:323/1‑148 (MQ=255)
                                                                                                cAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAAc                                                    >  3:98419/1‑148 (MQ=255)
                                                                                                  aGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCAt                                                 >  6:211932/1‑149 (MQ=255)
                                                                                                   gggCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATg                                                <  7:98292/149‑1 (MQ=255)
                                                                                                   gggCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATg                                                >  2:17253/1‑149 (MQ=255)
                                                                                                            tACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTcgac                                       <  7:67046/149‑1 (MQ=255)
                                                                                                                         gCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCTAGCCCGAAACCATGCAGGt                                           <  3:61311/132‑1 (MQ=255)
                                                                                                                         gCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCTAGCCCGAAACCATGCAGGt                                           <  3:57676/132‑1 (MQ=255)
                                                                                                                         gCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCa                            <  6:13315/147‑1 (MQ=255)
                                                                                                                         gCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCAAGCCCGAAACCATGCAGGt                                           >  4:61311/1‑132 (MQ=255)
                                                                                                                         gCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACAAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCAAGCCCGAAAACATGCAGGt                                           >  4:57676/1‑132 (MQ=255)
                                                                                                                             actgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCTTGCAGGTCGACGATCGCCGCCACGAt                        <  4:98419/147‑1 (MQ=255)
                                                                                                                              ctgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCg                      <  4:323/148‑1 (MQ=255)
                                                                                                                               tgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGa                     >  5:2533/1‑148 (MQ=255)
                                                                                                                               tgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGa                     >  3:62905/1‑148 (MQ=255)
                                                                                                                                               gCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGc    >  2:87577/1‑149 (MQ=255)
                                                                                                                                                cACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCACTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGc    <  8:169901/148‑1 (MQ=255)
                                                                                                                                                 aCCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGCCg  >  6:52092/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGCCG  >  NC_002947/3304618‑3304911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑