Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,342,964 G→A 9.0% Q15* (CAG→TAG)  yefM ← YoeB‑YefM toxin‑antitoxin system antitoxin/DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,342,9640GA 9.0% 120.1 / 2.9 45Q15* (CAG→TAG) yefMYoeB‑YefM toxin‑antitoxin system antitoxin/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base A (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

AGCGCGAAGCGTTTTTGGACGATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGAAGCCTGTCGACCCGCACGGCG  >  NC_002947/3342817‑3343108
                                                                                                                                                   |                                                                                                                                                
aGCGCGAAGCGTTTTTGGACGATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGc                                                                                                                                                 <  2:85176/149‑1 (MQ=255)
 gcgcGAAGCGTTTTTGGACGATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCt                                                                                                                                                >  5:53987/1‑149 (MQ=255)
                 gACGATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGc                                                                                                                                >  4:52424/1‑149 (MQ=255)
                    gATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTACTTCAGTTCGGCGCGAGCCTg                                                                                                                             >  4:87384/1‑149 (MQ=255)
                    gATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTACTTCAGTTCGGCGCGAGCCTg                                                                                                                             >  2:202163/1‑149 (MQ=255)
                     aTCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTgg                                                                                                                            <  1:107392/149‑1 (MQ=255)
                           aCAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCCTCAGTTCGGCGCGAGCCTGGCTaaaa                                                                                                                      <  3:101581/149‑1 (MQ=255)
                           aCAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTACTTCAGTTCGGCGCGAGCCTGGCTaaaa                                                                                                                      <  3:87384/149‑1 (MQ=255)
                           aCAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTACTTCAGTTCGGCGCGAGCCTGGCTaaaa                                                                                                                      <  1:202163/149‑1 (MQ=255)
                               gTGAATGGTCTCGTTCATCCCGTTGTAGTGCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCa                                                                                                                  <  5:72624/149‑1 (MQ=255)
                                 gAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAga                                                                                                                    >  6:8168/1‑144 (MQ=255)
                                 gAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAga                                                                                                                    <  5:8168/145‑2 (MQ=255)
                                      gTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTGCGGGTTCGCCACGCTGTAGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTAGGCGCGAGCCTGGCTAAAAGTCAGTACGTg                                                                                                           >  2:218584/1‑149 (MQ=255)
                                         tcGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGt                                                                                                                <  6:177142/141‑1 (MQ=255)
                                         tcGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGt                                                                                                                >  5:177142/1‑141 (MQ=255)
                                           gTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCAt                                                                                                        <  2:267548/147‑1 (MQ=255)
                                            gatgtCCCGTTGTAGTCCTCCAGAGACATGATCACTACGGTTTCGCCTCGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAAGATCATCCATTGTATGCTTCAGTTCGGCGCTAGCCTGGCTAAAAGTCAGTACGTGCATTTg                                                                                                     <  5:63745/145‑1 (MQ=255)
                                                           tcctccAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGct                                                                                      >  4:19625/1‑149 (MQ=255)
                                                             ctccAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGctct                                                                                    <  3:52424/149‑1 (MQ=255)
                                                             ctccAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCGCCAGGctct                                                                                    >  2:257032/1‑149 (MQ=255)
                                                              tccAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTg                                                                                   >  5:207455/1‑149 (MQ=255)
                                                              tccAGAGACAACAACACTACGCGTTCGCCACCCTGACGCTTGATTACGGCGGGCTCATGGTCCCGGCAAAAATCATCCATTGTCTGCTTCAGTTCGGCGCGACCCTGGCTAAAAGTCAGTACGTGCATTTGGCCATCCTCCAGCctcag                                                                                   >  6:266415/1‑147 (MQ=255)
                                                               ccAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCt                                                                                                                                                >  3:176607/1‑87 (MQ=255)
                                                               ccAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCt                                                                                                                                                <  4:176607/87‑1 (MQ=255)
                                                                    gaCATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCa                                                                                                 <  8:130900/129‑1 (MQ=255)
                                                                    gaCATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCa                                                                                                 >  7:130900/1‑129 (MQ=255)
                                                                          atcaCTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAgg                                                                        <  3:19625/148‑1 (MQ=255)
                                                                                          cACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTaa                                                                          >  5:221575/1‑130 (MQ=255)
                                                                                          cACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTaa                                                                          <  6:221575/130‑1 (MQ=255)
                                                                                               tGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAg                                                  >  3:190618/1‑149 (MQ=255)
                                                                                                   gcgTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGa                                              <  6:53987/149‑1 (MQ=255)
                                                                                                        aTTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTACAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCAACAGGTGACGcc                                          >  7:252207/1‑148 (MQ=255)
                                                                                                             ggcggGCTCATGGTCCCGGCAAACATCATCCCTTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGt                                    <  6:182917/149‑1 (MQ=255)
                                                                                                              gcggGCTCATGGTCCCGGCAAACTTCATCCATTGTCTTCTTCAGTTCGGCGCGAGCCTGTCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGtt                                   <  5:266415/149‑1 (MQ=255)
                                                                                                                  gCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCGCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTaa                               <  1:257032/149‑1 (MQ=255)
                                                                                                                    tCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAg                              >  8:174989/1‑148 (MQ=255)
                                                                                                                      aTGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGa                             <  6:207455/147‑1 (MQ=255)
                                                                                                                          tCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGa                       <  7:174989/149‑1 (MQ=255)
                                                                                                                             cGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGaa                      <  3:195981/147‑1 (MQ=255)
                                                                                                                             cGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGaa                      >  4:195981/1‑147 (MQ=255)
                                                                                                                             cGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGAAGc                    >  4:48341/1‑149 (MQ=255)
                                                                                                                                cAAACATCAACCAGTGTGCGCTTCACTTCGGCGCGACCCGGGCTAAAACTCACTACGTCCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTCACGCCCAAAGTTCTAAGAAAGTGAAGCCTg                 <  4:9650/149‑1 (MQ=255)
                                                                                                                                      taatcCATTGTCTGCTTCAGTTCGTCGCGAGCCTGGCTAAAAGTCAGAACGTGCATTTGTCCATCCTACAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGAAGCCTGTCGAcc           <  8:252207/147‑1 (MQ=255)
                                                                                                                                         tcCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGAAGCCTGTCGACCCg         >  1:199489/1‑148 (MQ=255)
                                                                                                                                               gTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGAAGCCTGTCGACCCGCAcggcg  >  7:68555/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGCGCGAAGCGTTTTTGGACGATCCCAACAGGTGAATGGTCTCGTTCATCCCGTTGTAGTCCTCCAGAGACATCATCACTACGGGTTCGCCACGCTGTCGCGTGATTACGGCGGGCTCATGGTCCCGGCAAACATCATCCATTGTCTGCTTCAGTTCGGCGCGAGCCTGGCTAAAAGTCAGTACGTGCATTTGTCCATCCTCCAGGCTCTGCCCGGGTAAGGGTTGCTCTTTGGACCGCCACAGGTGACGCCCAAAGTTCTAAGAAAGTGAAGCCTGTCGACCCGCACGGCG  >  NC_002947/3342817‑3343108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑