Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,380,705 T→C 10.0% L126P (CTT→CCT)  tnpT‑I → cointegrate resolution protein T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,380,7050TC10.0% 135.8 / 5.3 50L126P (CTT→CCT) tnpT‑Icointegrate resolution protein T
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base C (3/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAG  >  NC_002947/3380558‑3380844
                                                                                                                                                   |                                                                                                                                           
aTCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCtt                                                                                                                                            >  1:33907/1‑149 (MQ=255)
    gCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg                                                                                                                                        >  6:219117/1‑149 (MQ=255)
          ggaggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTc                                                                                                                                  <  1:78787/149‑1 (MQ=255)
          ggaggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCCTGAAGCTGTTc                                                                                                                                  <  2:224687/149‑1 (MQ=255)
                     aGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCaa                                                                                                                             >  1:118787/1‑143 (MQ=255)
                     aGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCaa                                                                                                                             <  2:118787/143‑1 (MQ=255)
                     aGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTa                                                                                                                       <  4:186098/149‑1 (MQ=255)
                       gCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg                                                                                                                                        <  7:134361/130‑1 (MQ=255)
                       gCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg                                                                                                                                        >  8:134361/1‑130 (MQ=255)
                           ccGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTTCCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAcagcag                                                                                                                 >  1:190302/1‑149 (MQ=255)
                                 cccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcag                                                                                                           <  1:204614/149‑1 (MQ=255)
                                  ccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAca                                                                                                                     >  3:120260/1‑138 (MQ=255)
                                  ccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAca                                                                                                                     <  4:120260/138‑1 (MQ=255)
                                   cAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcagca                                                                                                         <  6:272848/149‑1 (MQ=255)
                                   cAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcag                                                                                                           >  5:251741/1‑147 (MQ=255)
                                        gcgcGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCTGCAAGCGCTACAGCAGGAACAACAGCAAc                                                                                                    >  3:197890/1‑149 (MQ=255)
                                                aacTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCaa                                                                                               >  7:226853/1‑146 (MQ=255)
                                                   tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGAcc                                                                                         >  5:1351/1‑149 (MQ=255)
                                                      aaCGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAg                                                                                      <  6:1351/149‑1 (MQ=255)
                                                        cgcgAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCaa                                                                                               <  4:229829/138‑1 (MQ=255)
                                                        cgcgAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCaa                                                                                               >  3:229829/1‑138 (MQ=255)
                                                         gcgAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGtt                                                                                   >  6:245463/1‑149 (MQ=255)
                                                           gAACGGCTAGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTga                                                                                 <  2:235998/149‑1 (MQ=255)
                                                                  tCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTgaga                                                                               >  4:51301/1‑144 (MQ=255)
                                                                  tCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTgaga                                                                               <  3:51301/144‑1 (MQ=255)
                                                                         ccAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcagc                                                                                                          >  8:17395/1‑110 (MQ=255)
                                                                         ccAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcagc                                                                                                          <  7:17395/110‑1 (MQ=255)
                                                                          cAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGgc                                                                  <  4:127445/149‑1 (MQ=255)
                                                                           aGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGc                                                                                                  >  3:250272/1‑116 (MQ=255)
                                                                           aGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGc                                                                                                  <  4:250272/116‑1 (MQ=255)
                                                                           aGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGgcg                                                                 <  5:219117/149‑1 (MQ=255)
                                                                               ccAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAgg                                                                                                                <  8:174248/98‑1 (MQ=255)
                                                                               ccAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAgg                                                                                                                >  7:174248/1‑98 (MQ=255)
                                                                                   gCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTaa                                                         <  2:190302/149‑1 (MQ=255)
                                                                                    cTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAAc                                                        <  6:251741/149‑1 (MQ=255)
                                                                                       ggCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACgg                                                      <  6:126766/148‑1 (MQ=255)
                                                                                       ggCTGGCCGAAAGCAGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACgg                                                      <  8:137563/148‑1 (MQ=255)
                                                                                         cTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGa                                                   <  5:245463/149‑1 (MQ=255)
                                                                                              cGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCagg                                              <  8:226853/149‑1 (MQ=255)
                                                                                                      gAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTgcgc                                      >  5:241411/1‑149 (MQ=255)
                                                                                                            agcagTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTg                                   >  6:224310/1‑146 (MQ=255)
                                                                                                            agcagTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCa                                 <  5:224310/148‑1 (MQ=255)
                                                                                                                    gAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGCCCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGa                        >  6:253739/1‑149 (MQ=255)
                                                                                                                    gAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGa                        >  2:114751/1‑149 (MQ=255)
                                                                                                                      aaGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCTGCAAGCGCTACAGCAGGAACAACAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACa                      <  4:197890/149‑1 (MQ=255)
                                                                                                                               cagcGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAACAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATtc             >  8:113505/1‑149 (MQ=255)
                                                                                                                                       tCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTg     >  4:56034/1‑149 (MQ=255)
                                                                                                                                         gctgAACAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTg     >  8:11298/4‑147 (MQ=255)
                                                                                                                                           tgAACAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag  >  5:33738/2‑148 (MQ=255)
                                                                                                                                             aaCAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTTAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATcg                           <  3:159316/121‑1 (MQ=255)
                                                                                                                                             aaCAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATcg                           >  4:159316/1‑121 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
ATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAG  >  NC_002947/3380558‑3380844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑