Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,380,707 C→G 9.8% Q127E (CAG→GAG) ‡ tnpT‑I → cointegrate resolution protein T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,380,7070CG 9.8% 151.5 / 7.3 51Q127E (CAG→GAG) ‡tnpT‑Icointegrate resolution protein T
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base G (3/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATC  >  NC_002947/3380562‑3380854
                                                                                                                                                 |                                                                                                                                                   
gCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg                                                                                                                                                  >  6:219117/1‑149 (MQ=255)
      ggaggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTc                                                                                                                                            <  1:78787/149‑1 (MQ=255)
      ggaggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCCTGAAGCTGTTc                                                                                                                                            <  2:224687/149‑1 (MQ=255)
                 aGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCaa                                                                                                                                       >  1:118787/1‑143 (MQ=255)
                 aGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCaa                                                                                                                                       <  2:118787/143‑1 (MQ=255)
                 aGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTa                                                                                                                                 <  4:186098/149‑1 (MQ=255)
                   gCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg                                                                                                                                                  <  7:134361/130‑1 (MQ=255)
                   gCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg                                                                                                                                                  >  8:134361/1‑130 (MQ=255)
                       ccGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTTCCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAcagcag                                                                                                                           >  1:190302/1‑149 (MQ=255)
                             cccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcag                                                                                                                     <  1:204614/149‑1 (MQ=255)
                              ccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAca                                                                                                                               <  4:120260/138‑1 (MQ=255)
                              ccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAca                                                                                                                               >  3:120260/1‑138 (MQ=255)
                               cAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcagca                                                                                                                   <  6:272848/149‑1 (MQ=255)
                               cAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcag                                                                                                                     >  5:251741/1‑147 (MQ=255)
                                    gcgcGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCTGCAAGCGCTACAGCAGGAACAACAGCAAc                                                                                                              >  3:197890/1‑149 (MQ=255)
                                            aacTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCaa                                                                                                         >  7:226853/1‑146 (MQ=255)
                                               tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGAcc                                                                                                   >  5:1351/1‑149 (MQ=255)
                                                  aaCGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAg                                                                                                <  6:1351/149‑1 (MQ=255)
                                                    cgcgAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCaa                                                                                                         >  3:229829/1‑138 (MQ=255)
                                                    cgcgAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCaa                                                                                                         <  4:229829/138‑1 (MQ=255)
                                                     gcgAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGtt                                                                                             >  6:245463/1‑149 (MQ=255)
                                                       gAACGGCTAGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTga                                                                                           <  2:235998/149‑1 (MQ=255)
                                                              tCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTgaga                                                                                         >  4:51301/1‑144 (MQ=255)
                                                              tCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTgaga                                                                                         <  3:51301/144‑1 (MQ=255)
                                                                     ccAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcagc                                                                                                                    <  7:17395/110‑1 (MQ=255)
                                                                     ccAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAAcagc                                                                                                                    >  8:17395/1‑110 (MQ=255)
                                                                      cAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGgc                                                                            <  4:127445/149‑1 (MQ=255)
                                                                       aGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGc                                                                                                            <  4:250272/116‑1 (MQ=255)
                                                                       aGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGc                                                                                                            >  3:250272/1‑116 (MQ=255)
                                                                       aGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGgcg                                                                           <  5:219117/149‑1 (MQ=255)
                                                                           ccAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAgg                                                                                                                          >  7:174248/1‑98 (MQ=255)
                                                                           ccAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAgg                                                                                                                          <  8:174248/98‑1 (MQ=255)
                                                                               gCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTaa                                                                   <  2:190302/149‑1 (MQ=255)
                                                                                cTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAAc                                                                  <  6:251741/149‑1 (MQ=255)
                                                                                   ggCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACgg                                                                <  6:126766/148‑1 (MQ=255)
                                                                                   ggCTGGCCGAAAGCAGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACgg                                                                <  8:137563/148‑1 (MQ=255)
                                                                                     cTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGa                                                             <  5:245463/149‑1 (MQ=255)
                                                                                          cGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCagg                                                        <  8:226853/149‑1 (MQ=255)
                                                                                                  gAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTgcgc                                                >  5:241411/1‑149 (MQ=255)
                                                                                                        agcagTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTg                                             >  6:224310/1‑146 (MQ=255)
                                                                                                        agcagTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCa                                           <  5:224310/148‑1 (MQ=255)
                                                                                                                gAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGCCCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGa                                  >  6:253739/1‑149 (MQ=255)
                                                                                                                gAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGa                                  >  2:114751/1‑149 (MQ=255)
                                                                                                                  aaGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCTGCAAGCGCTACAGCAGGAACAACAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACa                                <  4:197890/149‑1 (MQ=255)
                                                                                                                           cagcGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAACAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATtc                       >  8:113505/1‑149 (MQ=255)
                                                                                                                                   tCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTg               >  4:56034/1‑149 (MQ=255)
                                                                                                                                     gctgAACAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTg               >  8:11298/4‑147 (MQ=255)
                                                                                                                                       tgAACAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag            >  5:33738/2‑148 (MQ=255)
                                                                                                                                         aaCAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTTAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATcg                                     <  3:159316/121‑1 (MQ=255)
                                                                                                                                         aaCAGCCTGAAGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATcg                                     >  4:159316/1‑121 (MQ=255)
                                                                                                                                                tCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCAt   >  5:200936/1‑148 (MQ=255)
                                                                                                                                                 cAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCTATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATc  <  3:56034/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTGGCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATC  >  NC_002947/3380562‑3380854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑