Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,388,819 T→C 13.0% H287R (CAT→CGT) ‡ PP_2991 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,388,8190TC13.0% 114.7 / 7.8 46H287R (CAT→CGT) ‡PP_2991hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base C (3/3);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGACTTTTCCGGTCTGG  >  NC_002947/3388677‑3388965
                                                                                                                                              |                                                                                                                                                  
tttGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACg                                                                                                                                                >  4:230626/1‑147 (MQ=255)
tttGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCa                                                                                                                                              >  5:90907/1‑149 (MQ=255)
     gTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGc                                                                                                                                               <  6:11334/143‑1 (MQ=255)
     gTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGc                                                                                                                                               >  5:11334/1‑143 (MQ=255)
      tGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGc                                                                                                                                        >  8:114261/1‑149 (MQ=255)
      tGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCAga                                                                                                                                            >  1:148492/1‑144 (MQ=255)
      tGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCAga                                                                                                                                            <  2:148492/145‑2 (MQ=255)
            gagCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGaaa                                                                                                                                  <  6:90907/149‑1 (MQ=255)
                  aCTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCa                                                                                                                             >  4:9121/1‑148 (MQ=255)
                      tGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAAcc                                                                                                                        >  2:130506/1‑149 (MQ=255)
                      tGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCAga                                                                                                                                            >  1:240451/1‑128 (MQ=255)
                      tGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCATGGCTCGAGCCGCGACGCAga                                                                                                                                            <  2:240451/129‑2 (MQ=255)
                        aaaCGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCAt                                                                                                                            <  1:135051/143‑1 (MQ=255)
                        aaaCGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCAt                                                                                                                            >  2:135051/1‑143 (MQ=255)
                            gCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCg                                                                                                                                       >  2:186550/1‑128 (MQ=255)
                                 cACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACt                                                                                                             <  1:130506/149‑1 (MQ=255)
                                    gTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGc                                                                                                          <  4:44530/149‑1 (MQ=255)
                                    gTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGc                                                                                                          <  4:48223/149‑1 (MQ=255)
                                     tATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGGGGGTGAATCTGCGTCGCGGCTCTAGCCATGACGCATTTGGCTGGAAACCGCATAACCGTATTCCAACTGGcc                                                                                                         <  2:43448/149‑1 (MQ=255)
                                      aTTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCg                                                                                                                               <  3:27476/126‑1 (MQ=255)
                                      aTTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCg                                                                                                                               >  4:27476/1‑126 (MQ=255)
                                          gTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCtga                                                                                                     <  2:118767/148‑1 (MQ=255)
                                             cTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTgatgat                                                                                                 >  3:261687/1‑149 (MQ=255)
                                                 atcatTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGTCCTGATGATc                                                                                                >  1:228041/1‑146 (MQ=255)
                                                 atcatTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATc                                                                                                <  2:228041/146‑1 (MQ=255)
                                                          cccAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGc                                                                                                                  >  6:1566/1‑119 (MQ=255)
                                                          cccAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGc                                                                                                                  <  5:1566/119‑1 (MQ=255)
                                                           ccAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAg                                                                                   >  6:25105/1‑149 (MQ=255)
                                                           ccAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAg                                                                                   <  5:25105/149‑1 (MQ=255)
                                                                          ggAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGCGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGat                                                                     >  6:170285/1‑148 (MQ=255)
                                                                          ggAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTgg                                                                                        >  3:14692/1‑129 (MQ=255)
                                                                          ggAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTgg                                                                                        <  4:14692/129‑1 (MQ=255)
                                                                                  ttCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGGTGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTc                                                            >  5:231944/1‑149 (MQ=255)
                                                                                      aCAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGTga                                                        <  4:261687/149‑1 (MQ=255)
                                                                                                gTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGt                                              <  3:230626/149‑1 (MQ=255)
                                                                                                gTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGt                                              >  8:60043/1‑149 (MQ=255)
                                                                                                    ccTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCga                                                        <  4:173140/135‑1 (MQ=255)
                                                                                                    ccTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCga                                                        >  3:173140/1‑135 (MQ=255)
                                                                                                           tgAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCttcttc                                   <  7:60043/149‑1 (MQ=255)
                                                                                                               ggTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCtt                               <  7:114261/149‑1 (MQ=255)
                                                                                                                  ggCTGAAGCTGCGGCGCGGCTCTAGCCATGACGCAGTTGGCGGGAACCCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGa                            <  4:65541/149‑1 (MQ=255)
                                                                                                                   gCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGaa                           <  3:9121/149‑1 (MQ=255)
                                                                                                                     tGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAg                          >  3:131635/1‑148 (MQ=255)
                                                                                                                                           ccATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGACTTTTCCGGTCTg   >  3:229477/1‑149 (MQ=255)
                                                                                                                                            cATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGACTTTTCCGGTCTgg  <  5:170285/149‑1 (MQ=255)
                                                                                                                                            cATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGACTTTTCCGGTCTgg  <  6:231944/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TTTGGGTGCATAGAGCGCACTCTGAAACGCTGGCACGTATTCGTACTTCATCATTTTCCCCAACTTCAGGGTGCGGAGGTAATTCGACAGAGCGCCGTCGCCTAGTGTGAGGGTGGCTGAATCTGCGTCGCGGCTCGAGCCATGACGCAGTTGGCGGGAAACCGCATAACCGTATGCCAACTGGCCCTGATGATCGAGATTGGTAAAGCTGCTGGTGACGATATCGACTTCGCGAGAGAGCTTGTCGAGCTTCTTCGCTTTGAAGGACACGTCGACTTTTCCGGTCTGG  >  NC_002947/3388677‑3388965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑