Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,430,575	T→G	18.4%	L265V (TTG→GTG)	PP_3045 →	ATP‑dependent protease ClpP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,430,575	0	T	G	18.4%	58.2 / 8.0	27	L265V (TTG→GTG)	PP_3045	ATP‑dependent protease ClpP
Reads supporting (aligned to +/- strand):  ref base T (11/11);  new base G (3/2);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAG  >  NC_002947/3430427‑3430713                                                                                                                                                     |                                                                                                                                           gATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCttgt                                                                                                                                            <  4:179795/149‑1 (MQ=255)       gcgTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCa                                                                                                                                      <  1:41035/149‑1 (MQ=255)                 aaTACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTGGGTGATCAACAAGGccaa                                                                                                                            <  5:162823/149‑3 (MQ=255)                   tACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTgca                                                                                                                          <  2:215847/149‑1 (MQ=255)                                      gATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCaa                                                                                                       >  3:113833/1‑149 (MQ=255)                                          aGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTCGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCtg                                                                                                   <  5:79046/149‑1 (MQ=255)                                                            aTGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGa                                                                                                              <  1:68240/120‑1 (MQ=255)                                                            aTGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGa                                                                                                              >  2:68240/1‑120 (MQ=255)                                                                  tGACCGAGCCGGTTGATACCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCATTGGCATTCTTCATCACACAGGCCTGCAGCAAGGCGGGCATCAGCAACCTGGTGGAGCCGCTGATTGCATCGAc                                                                            >  7:114085/1‑148 (MQ=255)                                                                        aGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCGGCTGATTGCATCGACCAAGCtt                                                                     >  4:3986/1‑149 (MQ=255)                                                                        aGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCtt                                                                     <  4:175873/149‑1 (MQ=255)                                                                         gCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCa                                                                                                           <  6:202441/110‑1 (MQ=255)                                                                         gCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCa                                                                                                           >  5:202441/1‑110 (MQ=255)                                                                                              cAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTgcagg                                               <  4:113833/149‑1 (MQ=255)                                                                                                   aGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGgcagca                                          >  6:78866/1‑149 (MQ=255)                                                                                                        gCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGAc                                     >  7:2281/1‑149 (MQ=255)                                                                                                            ccccAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCcgc                                 >  8:80986/1‑149 (MQ=255)                                                                                                              ccAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCt                                                                  >  7:52267/1‑114 (MQ=255)                                                                                                              ccAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCt                                                                  <  8:52267/114‑1 (MQ=255)                                                                                                                 gcgcCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCaa                            <  7:80986/149‑1 (MQ=255)                                                                                                                       aaTGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCgg                       >  7:106192/1‑148 (MQ=255)                                                                                                                                       cagcCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGtt      <  5:78866/149‑1 (MQ=255)                                                                                                                                       cagcCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGtt      <  8:114085/149‑1 (MQ=255)                                                                                                                                         ctgcaggcctggGTGATCAACAAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGTGACCTCTGCGTTg     >  8:193896/6‑148 (MQ=255)                                                                                                                                         ctgcaggcctggGTGATCAACAAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTg     >  8:197545/6‑148 (MQ=255)                                                                                                                                            ttggccttgTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCAACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAg  >  8:133616/1‑148 (MQ=255)                                                                                                                                            caggcctggGTGATCAACAAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAg                                                           >  5:97144/3‑91 (MQ=38)                                                                                                                                            caggcctggGTGATCAACAAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAg                                                           <  6:97144/89‑1 (MQ=38)                                                                                                                                                     |                                                                                                                                           GATGGCGCGTTACCGCAATACCCCGCAAGCCCTATTGGATCAGCTCAAGGCCAGTAGGGATGGGGTGACCGAGCCGGTTGATCCCGAACCCGACAGCGAGCCAGCTCCCCCAGCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAG  >  NC_002947/3430427‑3430713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑