| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,466,843 | C→A | 17.6% | intergenic (‑18/+106) | PP_3081 ← / ← PP_5560 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,466,843 | 0 | C | A | 17.6% | 54.4 / 3.2 | 30 | intergenic (‑18/+106) | PP_3081/PP_5560 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (8/15); new base A (3/2); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTGGCTCCG > NC_002947/3466704‑3466991 | ggCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCGCAGGCGGCCaa < 5:215990/149‑1 (MQ=255)ggCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCGCAGGCGGCCaa < 3:265169/149‑1 (MQ=255) cAGGTTGTCCCAGCTAAGTTGCGGGGTCCGCTCTTCACCGGCTCCGGATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACTAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACg < 4:212757/149‑1 (MQ=255) gTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCtg > 7:5014/1‑149 (MQ=255) cccAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCCCCAGAGGCGGCCAACCCAGTGCAg > 8:219210/1‑149 (MQ=255) cGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAg > 5:234319/1‑149 (MQ=255) gCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGc < 7:219210/149‑1 (MQ=255) ctctTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCggcggc < 7:159777/114‑1 (MQ=255) ctctTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCggcggc > 8:159777/1‑114 (MQ=255) cACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCATTGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACTTCGCGCGTTGGCGGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAgt < 2:3447/147‑1 (MQ=255) cACCGGCACCGCATGCCGGGTCCGGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAgt < 2:224603/147‑1 (MQ=255) gCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGAGCCTTGGCCCCCGTAGGCCGCCAACGAGTAGCAAGCCAGCCAAGCAGCGAACAGCCAGAGTCTggg > 8:155276/1‑147 (MQ=255) cATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCAt < 6:138585/149‑1 (MQ=255) cGGGTCCTGGCCGACCTGTTGCATGGCAATGACGCGGCCATCTGTGAAAGACGAGTAACTGACCGACATCGCGCCTTGGCCGCCTGGGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACa < 4:33315/125‑1 (MQ=255) gCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTgg > 5:245806/1‑148 (MQ=255) cGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCCCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAg > 8:29215/1‑149 (MQ=255) gTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTgcggcg > 4:113018/1‑74 (MQ=255) gTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTgcggcg < 3:113018/74‑1 (MQ=255) gCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCa < 5:203618/149‑1 (MQ=255) gacATCAGTTAAAGACGATGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCTTCCAACGCTGTGCAGGGCAGCAAAGCAGCGATCAGCCAGTGTCTGAGGTGCATGTTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAg < 7:155276/147‑1 (MQ=255) aGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCACCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAg > 8:22290/1‑118 (MQ=255) aGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCACCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAg < 7:22290/118‑1 (MQ=255) aCGAGGAACTGACCGACATCGCGCATTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCgg < 3:63726/149‑1 (MQ=255) cTGACCGACATCGCGCCTTGGGCCCCAGCGGCGGCCAACCCGGTGGAGGGCAGCAAAGCAGCGACAAGCCAGTGTCGGGGGTGCATGGGCAATACATGGCAGGGCGTAACCGCGCAGTCTAGCCCCGGGCTGCTTAAGCGGCGGTCGTg > 8:9791/1‑149 (MQ=255) acacCGCCCCTTGCCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCg < 3:20989/149‑1 (MQ=255) cATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCgg < 6:234319/149‑1 (MQ=255) tCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCa < 7:234223/149‑1 (MQ=255) gcgcCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCa < 7:111960/114‑1 (MQ=255) gcgcCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGt < 6:245806/149‑1 (MQ=255) ttgttgggCCGCCGCTGGTGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGTTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGt < 7:52233/142‑1 (MQ=255) cgttGGCCGCCGCAGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAAAAGCCAGTGTCTGAGGTGCATGGTAAAACCTTGCCAGGCCGTCACCCCTCACTCTAGCCCACGTCTACTTCAGAGGCTATCCTGGTCCTAGGCAGt > 8:52233/3‑146 (MQ=255) gccTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGATGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTgg > 1:74542/1‑149 (MQ=255) cggcggCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTGGCTCCg < 7:169117/149‑1 (MQ=255) | GGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTGGCTCCG > NC_002947/3466704‑3466991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |