Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,475,491 G→T 22.1% H408N (CAC→AAC) ‡ PP_3087 ← excinuclease ABC subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,475,4910GT22.1% 33.7 / 3.2 18H408N (CAC→AAC) ‡PP_3087excinuclease ABC subunit A
Reads supporting (aligned to +/- strand):  ref base G (11/3);  new base T (2/2);  total (13/5)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CGCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGC  >  NC_002947/3475344‑3475628
                                                                                                                                                   |                                                                                                                                         
cgccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc                                                                                                                                          <  6:85350/149‑1 (MQ=255)
  ccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCaa                                                                                                                                        <  4:227165/149‑1 (MQ=255)
       ttCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgccgg                                                                                                                                   >  3:155822/1‑149 (MQ=255)
             ggCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca                                                                                                                             <  6:51573/149‑2 (MQ=255)
              gCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACgg                                                                                                                            >  1:124224/1‑149 (MQ=255)
                aGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCt                                                                                                                          >  5:132907/1‑149 (MQ=255)
                        cTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACCGTCGGCCGGCTTGCACCCGGCCGAcagtga                                                                                                                         >  8:73842/1‑137 (MQ=255)
                        cTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACCGTCGGCCGGCTTGCACCCGGCCGAcagtga                                                                                                                         <  7:73842/142‑6 (MQ=255)
                                accaGCCAGTCGGCGCGGCGCATGGTGTCCAGATCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCtcgtc                                                                                                                      >  1:33256/1‑137 (MQ=255)
                                    gccagTCGGCGCGGCGCATGCTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGCGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCCACGGCACGTCGAGTACGTACaccccc                                                                                                      >  6:6325/1‑149 (MQ=255)
                                                                                                    tttCAGGCGCTGCAGGGCTACAACCAGCGATTCACTGCCGGCCGGTTGCACGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACaa                                        <  1:166763/147‑1 (MQ=255)
                                                                                                        aGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCgg                                  >  3:170296/1‑149 (MQ=255)
                                                                                                          gcgcTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCgggg                                >  4:186586/1‑149 (MQ=255)
                                                                                                                      ttCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTc                      >  5:186547/1‑147 (MQ=255)
                                                                                                                                    cACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGACGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccg      >  5:274328/1‑149 (MQ=255)
                                                                                                                                       tGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGc                                                         >  3:36538/1‑95 (MQ=255)
                                                                                                                                        gTCGGCCGGGTGCAAGCCGGCCGACAGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAg             >  3:15254/1‑138 (MQ=255)
                                                                                                                                        gTCGGCCGGCTTGCACCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccgaggc  >  6:13632/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
CGCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGC  >  NC_002947/3475344‑3475628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑