Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,548,090 C→A 9.1% A345A (GCC→GCA)  PP_3132 → polysaccharide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,548,0900CA 9.1% 134.9 / 4.5 44A345A (GCC→GCA) PP_3132polysaccharide transporter
Reads supporting (aligned to +/- strand):  ref base C (18/22);  new base A (2/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.69e-01

CAAGCCGCGCCTGAAAGCGCTGTATTTCCGCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGACAACATCATCCGCCTGGTCTCGTTATGGGGGCTGTT  >  NC_002947/3547955‑3548230
                                                                                                                                       |                                                                                                                                            
cAAGCCGCGCCTGAAAGCGCTGTATTTCCGCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGTGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTgg                                                                                                                                 <  7:104442/149‑1 (MQ=255)
   gCCGCGCCTGAAAGCGCTGTATTTCCGCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGa                                                                                                                              <  1:17189/149‑1 (MQ=255)
             aaaGCGCTGTATTTCCGCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCg                                                                                                                      <  3:44618/147‑1 (MQ=255)
                 cgcTGTATTTCCGCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCg                                                                                                                >  8:142185/1‑149 (MQ=255)
                             gCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTg                                                                                                    >  5:65739/1‑149 (MQ=255)
                               tttCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTgcg                                                                                                  >  3:97267/1‑149 (MQ=255)
                                 tCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTgcgc                                                                                                 <  4:232684/148‑1 (MQ=255)
                                  cGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCta                                                                                               >  1:245139/1‑149 (MQ=255)
                                     ctgctgGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATAc                                                                                            >  2:38909/1‑149 (MQ=255)
                                        ctgGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATAcc                                                                                           <  1:38909/147‑1 (MQ=255)
                                          ggTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGAtt                                                                                                                            >  8:25278/1‑112 (MQ=255)
                                          ggTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGAtt                                                                                                                            <  7:25278/112‑1 (MQ=255)
                                           gTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGc                                                                                      >  7:201080/1‑149 (MQ=255)
                                            tCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATc                                                                                                          >  1:225524/1‑128 (MQ=255)
                                            tCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATc                                                                                                          <  2:225524/128‑1 (MQ=255)
                                                  tgctgGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCAGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCtcat                                                                                  <  3:248311/146‑1 (MQ=255)
                                                  tgctgGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCAGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCtcat                                                                                  <  3:252293/146‑1 (MQ=255)
                                                  tgctgGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCAGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCtcat                                                                                  >  4:248311/1‑146 (MQ=255)
                                                  tgctgGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCAGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCtcat                                                                                  >  4:252293/1‑146 (MQ=255)
                                                    ctgGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGgt                                                                             <  7:142185/149‑1 (MQ=255)
                                                         gCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGa                                                                        >  8:84472/1‑149 (MQ=255)
                                                                   tgtgGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTgg                                                               <  8:201080/148‑1 (MQ=255)
                                                                    gtgGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTggg                                                              <  8:178986/148‑1 (MQ=255)
                                                                       gCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTg                                                                                        <  5:63119/119‑1 (MQ=255)
                                                                       gCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTg                                                                                        >  6:63119/1‑119 (MQ=255)
                                                                       gCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCt                                                           >  5:22874/1‑148 (MQ=255)
                                                                         ggTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGAc                                                        <  2:245139/149‑1 (MQ=255)
                                                                         ggTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGAc                                                        >  8:80449/1‑149 (MQ=255)
                                                                          gTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGAc                                                        >  7:2012/1‑148 (MQ=255)
                                                                             tCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCaa                                                     <  4:97267/148‑1 (MQ=255)
                                                                                tGACCGCCAGCCCGCTGCTGATAGGCTGGATGTATGACGATCGCTACCGCGAAGCCGCGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACAAAGTAc                                                 >  2:201653/1‑149 (MQ=255)
                                                                                      ccAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGc                                            >  1:211606/1‑148 (MQ=255)
                                                                                           ccGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGTTATCCTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGa                                      <  4:33388/149‑1 (MQ=255)
                                                                                            cGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGac                                     <  6:22874/149‑1 (MQ=255)
                                                                                            cGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGac                                     <  7:80449/149‑1 (MQ=255)
                                                                                                 ctgATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGacaaca                                 <  2:211606/148‑1 (MQ=255)
                                                                                                    aTCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGACAAcatc                               <  7:84472/147‑1 (MQ=255)
                                                                                                             aTGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGACAACATCATCCGCCTGGt                    >  1:13498/1‑149 (MQ=255)
                                                                                                                    aCGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGACAACATCATCCGCCTGGTCTCGtt              <  1:201653/148‑1 (MQ=255)
                                                                                                                         cGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTgg                                                               <  6:183401/94‑1 (MQ=255)
                                                                                                                         cGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTgg                                                               >  5:183401/1‑94 (MQ=255)
                                                                                                                               cgcgAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGACAACATCATCCGCCTGGTCTCGTTATGGGGGCTGtt  <  8:2012/149‑1 (MQ=255)
                                                                                                                                 cgAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTg                                                                <  2:158970/85‑1 (MQ=255)
                                                                                                                                 cgAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTg                                                                >  1:158970/1‑85 (MQ=255)
                                                                                                                                       |                                                                                                                                            
CAAGCCGCGCCTGAAAGCGCTGTATTTCCGCTTTCGGCTGCTGGTCGACCTGCTGGTGCTGTTCATTTGTGGCGGTTTCCTGACCGCCAGCCCGCTGCTGATCGGCTGGATGTATGACGATCGCTACCGCGAAGCCGGGCCGATGCTGGCGATTCTCTCGCTATCGTTCATCGTATTGCGCTATACCTTGGCTCATCAGGTGTGGATTGCCTTGGGCCTGACCAAGTACCAGGCCATGGACAACATCATCCGCCTGGTCTCGTTATGGGGGCTGTT  >  NC_002947/3547955‑3548230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑