Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,571,676 G→C 11.1% P235P (CCG→CCC)  yneJ → LysR family DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,571,6760GC11.1% 98.0 / 4.8 36P235P (CCG→CCC) yneJLysR family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (14/18);  new base C (2/2);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

CGCCTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTGAAG  >  NC_002947/3571529‑3571821
                                                                                                                                                    |                                                                                                                                                 
cGCCTTCCGCGCCAACTTTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCcgc                                                                                                                                                  <  2:250434/149‑1 (MQ=255)
  ccTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCgcgc                                                                                                                                                <  8:201044/149‑1 (MQ=255)
   cTTCCCCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgca                                                                                                                                               <  7:56793/149‑1 (MQ=255)
         cgcCAACTGT‑‑ATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCATggcc                                                                                                                                       <  2:110487/149‑4 (MQ=255)
           ccAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTc                                                                                                                                       <  3:185323/149‑1 (MQ=255)
            cAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCATggcca                                                                                                                                      <  4:68922/149‑5 (MQ=255)
              aCTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCACTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGAc                                                                                                                                    <  7:137052/149‑1 (MQ=255)
                  ttCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgca                                                                                                                                               >  4:62779/1‑134 (MQ=255)
                  ttCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgca                                                                                                                                               <  3:62779/134‑1 (MQ=255)
                      tACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGcc                                                                                                                            >  8:183101/1‑149 (MQ=255)
                         cGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCgg                                                                                                                         <  3:119476/149‑1 (MQ=255)
                             aTCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAAACGGAGTAGTACCACGGGATTTTGGCCCGCCTAATTGCCGGTGCCGGCCTGGCCATTCTGCCCCGCAGCATGCTGCACAAAATGCCCGGGTg                                                                                                                      >  1:212527/1‑149 (MQ=255)
                             aTCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTgc                                                                                                                     <  7:180224/149‑1 (MQ=255)
                                                   gCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCaa                                                                                               >  1:103539/1‑149 (MQ=255)
                                                       gACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCt                                                                                                                                        <  4:23456/104‑1 (MQ=255)
                                                       gACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCt                                                                                                                                        >  3:23456/1‑104 (MQ=255)
                                                           aGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCggag                                                                                       <  6:120593/149‑1 (MQ=255)
                                                                        cAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATc                                                                          <  2:103539/149‑1 (MQ=255)
                                                                        cAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATc                                                                          <  7:183101/149‑1 (MQ=255)
                                                                                aCG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAcc                                                                  >  1:160246/1‑149 (MQ=255)
                                                                                  g‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctg                                                                >  5:13912/1‑149 (MQ=255)
                                                                                            tCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATg                                                       >  4:56999/1‑149 (MQ=255)
                                                                                                 ccACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcga                                                   <  3:56999/148‑1 (MQ=255)
                                                                                                        aTGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTAc                                           <  2:7196/149‑1 (MQ=255)
                                                                                                          gTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATg                                                       <  4:123212/135‑1 (MQ=255)
                                                                                                          gTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATg                                                       >  3:123212/1‑135 (MQ=255)
                                                                                                          gTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACgg                                         >  2:180838/1‑149 (MQ=255)
                                                                                                               ccTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTcg                                    <  1:180838/149‑1 (MQ=255)
                                                                                                                 tGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgacga                                                >  7:107421/1‑135 (MQ=255)
                                                                                                                 tGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgacga                                                <  8:107421/135‑1 (MQ=255)
                                                                                                                         tGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGa                          <  2:212527/149‑1 (MQ=255)
                                                                                                                         tGCGGGTGCCGGCCTGGCCAAGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGttt                            >  3:189969/1‑147 (MQ=255)
                                                                                                                              gTGCCGGACTGTCCATGCTGCCGCGCAGAATTCTCGACAATATGGCCGGGTGCAGCACCGTCTGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGTCGAGGTACGGtctc                                     <  4:84954/133‑1 (MQ=255)
                                                                                                                                         agcATGCTGCGCGGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGtctc                                     >  7:218370/3‑122 (MQ=255)
                                                                                                                                           cATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGc        >  2:245008/1‑149 (MQ=255)
                                                                                                                                            aTGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCt       <  2:160246/149‑1 (MQ=255)
                                                                                                                                                 gcCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTg     >  1:247408/1‑146 (MQ=255)
                                                                                                                                                  ccGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTGTGAGCATGTTCGTGAAGTTGCTTgaag  >  6:269140/1‑148 (MQ=255)
                                                                                                                                                   cgcgCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAg            >  7:241218/1‑137 (MQ=255)
                                                                                                                                                   cgcgCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAg            <  8:241218/137‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGCCTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACG‑AAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTGAAG  >  NC_002947/3571529‑3571821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑