Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,835,761 A→C 9.9% Q83H (CAA→CAC)  PP_3387 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,835,7610AC 9.9% 115.2 / 3.0 40Q83H (CAA→CAC) PP_3387hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/20);  new base C (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

AAGAACAACTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCACGGACGGCCACTCGTACCTGTTGCGCTTCCACACCGCCGCGG  >  NC_002947/3835616‑3835906
                                                                                                                                                 |                                                                                                                                                  
aaGAACAACTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCGGTGGCAACtt                                                                                                                                                 <  2:34847/149‑1 (MQ=255)
aaGAACAACTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGCAGCACCtt                                                                                                                                                 >  8:84094/1‑149 (MQ=255)
aaGAACAACTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGCAGCACCtt                                                                                                                                                 >  4:39368/1‑149 (MQ=255)
  gAACAACTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTg                                                                                                                                                >  5:180229/1‑148 (MQ=255)
        cTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAgg                                                                                                                                         <  3:137091/149‑1 (MQ=255)
          gACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGtg                                                                                                                                       >  5:208390/1‑149 (MQ=255)
           aCCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGtg                                                                                                                                       <  3:130912/148‑1 (MQ=255)
                   cTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGCAGCACCTTGTTCAAGTTGCCACTggaa                                                                                                                              <  3:39368/149‑3 (MQ=255)
                   cTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGCAGCACCTTGTTCAAGTTGCCACTggaa                                                                                                                              <  7:84094/149‑3 (MQ=255)
                     aaacGGGCCGCTGCTGGCCACTGGTGCAGAAAGCCCAGTTCAGCAACCTGCGGACCGAAGGCGCGTGGGGGTGCGGGGCGCGCCGCGGTTACGATGGCTAGGCCCAGTACGACTTCCAGTGGGAACGAGAAAAAGGTGCTGATTGCGcc                                                                                                                            >  4:150152/3‑149 (MQ=255)
                       acGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑gt                                                                                                                         <  5:138424/149‑1 (MQ=255)
                           gCCGGTGCTGGCGACTGCTGCCGCAAGCGCAGTTAAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCTCGCATAGGTTCCGAAGTCTCGGCCCAGTACGACTTCAAGTGGAAAATTGAAAATGGTTCTTCTTGCGCC‑gtgttc                                                                                                                     >  1:193399/1‑147 (MQ=255)
                           gCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGc                                                                                                                     >  1:100649/1‑149 (MQ=255)
                               ctgctgGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCt                                                                                                                 <  3:150152/149‑1 (MQ=255)
                                  ctgGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGa                                                                                                              <  6:208390/149‑1 (MQ=255)
                                   tgGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGa                                                                                                              >  4:97489/1‑148 (MQ=255)
                                    ggCCACTGCTGCAGGAAGCCCAGTTCAGCAGCCTGCGGACAGCAGGCCCTTGGCTGCTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGCTGCTGCTGGCGCC‑GTGTGCGGCTGGATa                                                                                                            <  5:245477/149‑1 (MQ=255)
                                          tgctgcATCAAGGCCAGTTCTGCAACCTGCGGACAGCATGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGgc                                                                                                                               <  8:252493/125‑1 (MQ=255)
                                          tgctgcAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGgc                                                                                                                               >  7:252493/1‑125 (MQ=255)
                                                  caAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGTCGCC‑GTGTGCGGCTGGATACTCAGCGCGCTTcc                                                                                              >  5:127320/1‑149 (MQ=255)
                                                    aGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCg                                                                                            <  6:180229/149‑1 (MQ=255)
                                                       ccAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCATCGCGCTTCCCGCTg                                                                                         <  6:64706/149‑1 (MQ=255)
                                                             cAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGTTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCgg                                                                                    <  2:193399/148‑1 (MQ=255)
                                                             cAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCgg                                                                                    <  6:15624/148‑1 (MQ=255)
                                                                              cagGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCt                                                                                                                 <  1:231545/102‑1 (MQ=255)
                                                                              cagGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCt                                                                                                                 >  2:231545/1‑102 (MQ=255)
                                                                                ggCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAGCAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCa                                                                <  1:172216/149‑1 (MQ=255)
                                                                                            ttcggttcgCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGt                                                    >  5:59732/1‑149 (MQ=255)
                                                                                              cggttcgCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTc                                                   <  3:97489/148‑1 (MQ=255)
                                                                                              cggttcgCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGAGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCACCATCGTCa                                                  >  1:197086/1‑149 (MQ=255)
                                                                                              cgggtcgCGCCTCGGTTCCGATGTCTCTGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTc                                                   <  3:150646/148‑1 (MQ=255)
                                                                                               ggttcgCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCa                                                  <  6:127320/148‑1 (MQ=255)
                                                                                                           ggTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCacgg                                         <  5:71266/145‑1 (MQ=255)
                                                                                                           ggTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCacgg                                         >  6:71266/1‑145 (MQ=255)
                                                                                                                 gATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCacggacgg                                     >  1:116573/1‑143 (MQ=255)
                                                                                                                 gATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCacggacgg                                     <  2:116573/143‑1 (MQ=255)
                                                                                                                 gATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCACGGACGGCCACTc                               >  3:23777/1‑149 (MQ=255)
                                                                                                                      ctcGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCACGGACGGCCACTCGTAcc                          >  3:151906/1‑149 (MQ=255)
                                                                                                                       tcGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCCGGGGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCACGGACGGCCACTCGTAcc                          >  1:70889/1‑149 (MQ=255)
                                                                                                                                              gCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCACGGACGGCCACTCGTACCTGTTGCGCTTCCACACCGCCGCgg  <  7:243983/149‑1 (MQ=255)
                                                                                                                                                 aCTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACgg                                               >  4:239826/1‑101 (MQ=255)
                                                                                                                                                 aCTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACgg                                               <  3:239826/101‑1 (MQ=255)
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AAGAACAACTGACCGGGCACTACACGGGCCGCTGCTGGCCACTGCTGCAGCAAGCCCAGTTCAGCAACCTGCGGACAGCAGGCCCTTGGTTGTTCGGTTCGCGCCTCGGTTCCGATGTCTCGGCCCAGTACGACTTCCAGTGGCAACTTGAACAAGGTGCTGCTGGCGCC‑GTGTGCGGCTGGATAGTCAGCGCGCTTCCCGCTGCCCGGCTCGCCGGACACTTGAGCCACGCCAACATCGTCACGGGCACGGACGGCCACTCGTACCTGTTGCGCTTCCACACCGCCGCGG  >  NC_002947/3835616‑3835906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑