Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,867,946 T→C 11.1% I71T (ATC→ACC)  gntT → D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,867,9460TC11.1% 92.4 / 2.9 36I71T (ATC→ACC) gntTD‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (19/13);  new base C (2/2);  total (21/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCGATGATGTTCGCCGCCTTCCTGGTGGGCATCCCGCT  >  NC_002947/3867807‑3868093
                                                                                                                                            |                                                                                                                                                   
aTCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTc                                                                                                                                              <  3:260065/147‑1 (MQ=255)
  cACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGccc                                                                                                                                          >  1:170863/1‑149 (MQ=255)
    cccACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTg                                                                                                                                        >  1:194820/1‑149 (MQ=255)
          tCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCAc                                                                                                                                   >  6:261395/1‑148 (MQ=255)
           cAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCa                                                                                                                                 >  6:105476/1‑149 (MQ=255)
                       cTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGc                                                                                                                     <  4:62992/149‑1 (MQ=255)
                           gtcgCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGAt                                                                                                                 <  2:194820/149‑1 (MQ=255)
                                    aCCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTc                                                                                                        >  4:262201/1‑149 (MQ=255)
                                       cTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCGAAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCATGGGCACCATGATCGGCAAGCTGATGGCCGACTCCg                                                                                                      >  1:56505/1‑149 (MQ=255)
                                                  cTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATgg                                                                                                               <  5:25422/128‑1 (MQ=255)
                                                  cTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTAGGCAAGCTGATgg                                                                                                               >  6:25422/1‑128 (MQ=255)
                                                      cTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGAt                                                                                      <  2:170863/149‑1 (MQ=255)
                                                        gggCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCAcc                                                                                                                                  >  8:222724/1‑103 (MQ=255)
                                                        gggCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCAcc                                                                                                                                  <  7:222724/103‑1 (MQ=255)
                                                         tgCCTGACTTCCGTCATGCCGGTGGCC‑AAGGTCATGAAATCGGTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACAAGATcg                                                                                    <  5:251658/147‑1 (MQ=255)
                                                         ggCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATcgc                                                                                   >  4:211039/1‑149 (MQ=255)
                                                                   ccGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCt                                                                          <  3:117090/148‑1 (MQ=255)
                                                                    cGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCa                                                                        >  1:47642/1‑149 (MQ=255)
                                                                               tGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTc                                                             <  2:56505/149‑1 (MQ=255)
                                                                                ggCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTACCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCg                                                            >  6:128933/1‑149 (MQ=255)
                                                                                 gCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCg                                                            <  7:64693/148‑1 (MQ=255)
                                                                                  cc‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTACCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGc                                                          <  5:128933/149‑1 (MQ=255)
                                                                                     aaGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGc                                                          >  4:41941/1‑147 (MQ=255)
                                                                                         tCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCgg                                                           >  8:198201/1‑142 (MQ=255)
                                                                                         tCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCgg                                                           <  7:198201/142‑1 (MQ=255)
                                                                                                       cAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGc                                      >  1:75308/1‑149 (MQ=255)
                                                                                                        aGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGc                                      >  1:149191/1‑148 (MQ=255)
                                                                                                           aCGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCgatg                                  <  3:262201/149‑1 (MQ=255)
                                                                                                           aCGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCgatg                                  <  3:255648/149‑1 (MQ=255)
                                                                                                           aCGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCgat                                   >  4:104341/1‑148 (MQ=255)
                                                                                                                    gcggcgTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCGATGATGTTcgcc                         >  6:172023/1‑149 (MQ=255)
                                                                                                                                  ttCGTCGGTACCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCAtt                                          <  1:184756/118‑1 (MQ=255)
                                                                                                                                  ttCGTCGGTACCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCAtt                                          >  2:184756/1‑118 (MQ=255)
                                                                                                                                          tATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCGATGATGTTCGCCGCCTTCCTGGTGGGCATCCCGc   >  6:35707/1‑149 (MQ=255)
                                                                                                                                          tATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCGATGATGTTCGCCGCCTTCCTGGTGGGCATCCCGc   >  6:31689/1‑149 (MQ=255)
                                                                                                                                           aTCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGAGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCGATGATGTTCGCCGCCTTCCTGGTGGGCATCCCGCt  >  7:63407/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
ATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCC‑AAGGTCATGAAATCGTTCCAGGACGGTTTTGGCGGCGTACTCGGCTTCGTCGGTATCGTGCTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGACTCCGGTGGTGCCGACCAGATCGCGCAAACCCTCATCCGCGCCTTCGGCAAGAAGAACGTGCATTGGGCGATGATGTTCGCCGCCTTCCTGGTGGGCATCCCGCT  >  NC_002947/3867807‑3868093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑