Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,870,393 G→A 9.2% L176L (CTG→TTG)  PP_3419 ← sigma‑54 dependent transcriptional regulator/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,870,3930GA 9.2% 117.0 / 3.1 44L176L (CTG→TTG) PP_3419sigma‑54 dependent transcriptional regulator/response regulator
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base A (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

TTCTCGTGGCCAAACAGTTCGGCCTCAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTCGCGCTCCTGGTAGAACGA  >  NC_002947/3870245‑3870537
                                                                                                                                                    |                                                                                                                                                
tgcttgaggCCGAACTGTTTGGCCACAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAg                                                                                                                                                  >  3:62932/8‑149 (MQ=255)
                      ccTCAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCa                                                                                                                               >  5:230281/1‑146 (MQ=255)
                      ccTCAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCa                                                                                                                               <  6:230281/146‑1 (MQ=255)
                      ccTCAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTg                                                                                                                            >  2:128334/1‑149 (MQ=255)
                      ccTCAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTg                                                                                                                            <  3:76785/149‑1 (MQ=255)
                          aaGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCa                                                                                                                        >  2:75011/1‑149 (MQ=255)
                            gCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATg                                                                                                                      >  8:257/1‑149 (MQ=255)
                            gCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGAGCAGCACCGGTGGCAGGGCGTCACTGGCCATg                                                                                                                      >  6:194174/1‑149 (MQ=255)
                                      aGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCgg                                                                                                            >  5:144133/1‑149 (MQ=255)
                                           tCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCg                                                                                                       <  6:214760/149‑1 (MQ=255)
                                              aaGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAg                                                                                                     >  2:172913/1‑148 (MQ=255)
                                                gcgcAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCAc                                                                                                  <  4:205424/149‑1 (MQ=255)
                                                  gcAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCTGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCt                                                                                                <  5:194174/149‑1 (MQ=255)
                                                     aTTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAACACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGgc                                                                                             >  5:8454/1‑149 (MQ=255)
                                                     aTTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAACACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGgc                                                                                             >  5:4642/1‑149 (MQ=255)
                                                      ttGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGgcg                                                                                            >  2:205149/1‑149 (MQ=255)
                                                          aCTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAgg                                                                                        <  4:62932/149‑1 (MQ=255)
                                                          aCTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAgg                                                                                        <  1:128334/149‑1 (MQ=255)
                                                            tCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCa                                                                                          >  7:224415/1‑145 (MQ=255)
                                                            tCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCa                                                                                          <  8:224415/145‑1 (MQ=255)
                                                              gATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGgtgt                                                                                    >  1:31204/1‑149 (MQ=255)
                                                               aTGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTgg                                                                                                                           <  6:229517/109‑1 (MQ=255)
                                                               aTGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTgg                                                                                                                           >  5:229517/1‑109 (MQ=255)
                                                                    aGGCCCTTTACTGCGCGAAACATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGa                                                                              <  8:200925/149‑1 (MQ=255)
                                                                     ggCCCTTTACTGCGCGAACCATGGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAACACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAg                                                                             <  6:8454/149‑1 (MQ=255)
                                                                     ggCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAACACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAg                                                                             <  6:4642/149‑1 (MQ=255)
                                                                      gCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAgg                                                                            <  1:172913/149‑1 (MQ=255)
                                                                             aCTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGc                                                                      <  2:31204/148‑1 (MQ=255)
                                                                              cTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCAt                                                                    <  1:205149/149‑1 (MQ=255)
                                                                                    gAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGGCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCgg                                                              <  8:75419/149‑1 (MQ=255)
                                                                                     aaCCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGc                                                             <  7:257/149‑1 (MQ=255)
                                                                                       ccATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATc                                                                                                        >  7:102795/1‑104 (MQ=255)
                                                                                       ccATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATc                                                                                                        <  8:102795/104‑1 (MQ=255)
                                                                                             aaaTGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGcc                                                     >  5:40313/1‑149 (MQ=255)
                                                                                                        aCGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCt                                          <  6:144133/149‑1 (MQ=255)
                                                                                                        aCGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCt                                          >  5:93409/1‑149 (MQ=255)
                                                                                                              caccaGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAAcc                                    >  1:92214/1‑149 (MQ=255)
                                                                                                                 caGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCATCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCg                                  >  6:20516/1‑148 (MQ=255)
                                                                                                                          gCCAGTACCGGGCTCGCCGTCGATCAGCACCGGTGGCAGGGCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTgc                         <  5:221309/148‑1 (MQ=255)
                                                                                                                               tACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTcg                   >  4:105744/1‑149 (MQ=255)
                                                                                                                                         gCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCATCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTCGCGCTCCt            <  5:20516/146‑1 (MQ=255)
                                                                                                                                         gCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTCGCGCTCCTgg          >  1:217210/1‑148 (MQ=255)
                                                                                                                                          ccTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTCGCGCTCCTGGTa        <  1:75011/149‑1 (MQ=255)
                                                                                                                                           cTTAGATCATCACCGGTGGCATGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGGGCTTGATGGTGATCATCACCGGCTACTCGCCGATCAAAGCCTGCAACCCGGACTTCTTCGCCTCGCGCTCCTGGTAg       <  3:149873/149‑1 (MQ=255)
                                                                                                                                                 tCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTCGCGCTCCTGGTAGAACGa  >  6:81191/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTCTCGTGGCCAAACAGTTCGGCCTCAAGCAGGTTGGCAGGGATCGAAGCGCAATTGAACTCGATGAAAGGCCCTTTACTGCGCGAACCATCGAAATGCAGGGCACGGGCCACCAGTTCCTTGCCAGTACCGGTCTCGCCTTCGATCAGCACCGGTGGCAGGTCGTCACTGGCCATGCGCCGCTCGGCATCGAGCACCTGGCGCAGGGTGTGCTTGAGGGTGAGCATCACCGGCGACTCGCCGATCAAAGCCTGCAACCCGGACTTCTGCGCCTCGCGCTCCTGGTAGAACGA  >  NC_002947/3870245‑3870537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑