Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,924,613 A→G 8.3% D67D (GAT→GAC)  peaB ← quinohemoprotein amine dehydrogenase modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,924,6130AG 8.3% 132.0 / 2.7 48D67D (GAT→GAC) peaBquinohemoprotein amine dehydrogenase modification protein
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base G (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GCTGAGGTTGCAACCGGTGTTGACGTTGAGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGAACAGCATGCGGTCGGCA  >  NC_002947/3924469‑3924760
                                                                                                                                                 |                                                                                                                                                    
gCTGAGGTTGCAACCGGTGTTGACGTTGAGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCt                                                                                                                                                  >  8:46552/1‑149 (MQ=255)
          cAACCGGTGTTGACGTTGAGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCaaa                                                                                                                                        >  7:158278/1‑149 (MQ=255)
           aaCCGGTGTTGACGTTGAGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGAGATTTCAGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAAc                                                                                                                                       >  1:234690/1‑149 (MQ=255)
                gtgtTGACGTTGAGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAActgctg                                                                                                                                  <  7:146423/149‑1 (MQ=255)
                            aGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCt                                                                                                                       <  5:267766/148‑1 (MQ=255)
                                ccacGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCAt                                                                                                                   >  1:241176/1‑148 (MQ=255)
                                     gtgtTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑ctc                                                                                                            <  7:46552/149‑1 (MQ=255)
                                      tgtTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑ctcc                                                                                                           >  8:239281/1‑149 (MQ=255)
                                      tgtTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑ctcc                                                                                                           <  1:66390/149‑1 (MQ=255)
                                           aGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCgg                                                                                                       <  4:205819/148‑1 (MQ=255)
                                           aGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGc                                                                                                      >  3:24945/1‑149 (MQ=255)
                                            gcgcGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGcc                                                                                                     >  5:7034/1‑149 (MQ=255)
                                                      cTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCtt                                                                                           >  2:227231/1‑149 (MQ=255)
                                                       tCGACTTGTCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTg                                                                                          >  3:250755/1‑149 (MQ=255)
                                                       tCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTg                                                                                                                      <  5:262343/122‑1 (MQ=255)
                                                       tCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTg                                                                                                                      >  6:262343/1‑122 (MQ=255)
                                                              gCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGgc                                                                                     <  5:246097/147‑1 (MQ=255)
                                                               ccTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGgcgc                                                                                   >  7:66332/1‑148 (MQ=255)
                                                                       cGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTc                                                                           >  1:136888/1‑148 (MQ=255)
                                                                        gATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCga                                                                         >  7:22781/1‑149 (MQ=255)
                                                                        gATTTCCGGGGTACACGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCga                                                                         <  1:227231/149‑1 (MQ=255)
                                                                          tttCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCgatg                                                                       <  4:250755/149‑1 (MQ=255)
                                                                              cGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATgc                                                                   >  2:209883/1‑149 (MQ=255)
                                                                                 ggTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATgccgc                                                                <  4:26909/149‑1 (MQ=255)
                                                                                    cAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTg                                                                                                                      >  2:271042/1‑93 (MQ=255)
                                                                                    cAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTg                                                                                                                      <  1:271042/93‑1 (MQ=255)
                                                                                      gcggcgAGTCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATGTCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGt                                                            >  8:211342/1‑148 (MQ=255)
                                                                                      gcggcgAGTCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATGTCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTc                                                           >  8:207725/1‑149 (MQ=255)
                                                                                        ggcgAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCAc                                                         <  8:66332/149‑1 (MQ=255)
                                                                                           gAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACcgc                                                      <  2:136888/149‑1 (MQ=255)
                                                                                           gAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACcgc                                                      <  2:241176/149‑1 (MQ=255)
                                                                                                 tCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGc                                                >  8:29950/1‑149 (MQ=255)
                                                                                                         cAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACa                                        <  4:24945/149‑1 (MQ=255)
                                                                                                           gCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCTGGGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCACCCTTGATGGCGCAAGGTGTCGATGATGCCGCCGGt                                                            >  1:176256/1‑128 (MQ=255)
                                                                                                               cAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGaa                                   <  7:25554/148‑1 (MQ=255)
                                                                                                                aGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAAct                                 >  7:195937/1‑149 (MQ=255)
                                                                                                                    cgATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAActgctg                             <  1:209883/149‑1 (MQ=255)
                                                                                                                     gATCAGTTCGCGCAGGGTGTCCT‑GGATGTCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTgg                            <  7:211342/149‑1 (MQ=255)
                                                                                                                     gATCAGTTCGCGCAGGGTGTCCT‑GGATGTCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTgg                            <  7:207725/149‑1 (MQ=255)
                                                                                                                     gATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACCGCTgg                            <  7:239281/149‑1 (MQ=255)
                                                                                                                      aTCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTggg                           <  8:22781/149‑1 (MQ=255)
                                                                                                                       tCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAGACTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTggg                           >  1:215983/1‑149 (MQ=255)
                                                                                                                             cgcgCTGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGata                       >  4:193216/1‑146 (MQ=255)
                                                                                                                             cgcgCTGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGata                       <  3:193216/146‑1 (MQ=255)
                                                                                                                               cgcAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGaa                  >  7:70802/1‑149 (MQ=255)
                                                                                                                                     gtgtCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGAACAGCAt            <  7:29950/149‑1 (MQ=255)
                                                                                                                                          cT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGAACAGCATGCGGt       >  3:202610/1‑149 (MQ=255)
                                                                                                                                          cT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGAACAGCATGCGGt       >  8:54717/1‑149 (MQ=255)
                                                                                                                                                tatCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCTGCCGAGCAGCGTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCAGCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGAACAGCATGCGGTCGGCa  <  5:40532/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCTGAGGTTGCAACCGGTGTTGACGTTGAGCACCACGGTGTTGAGCGCGGTGCGCTCGACTTGCCTGAGGGCGATTTCCGGGGTCAGCGGCGAGCCGTCGCTGACCAGCTCCAGCGCGATCAGTTCGCGCAGGGTGTCCT‑GGATATCCTCGCCGGCAAACTGCTGGCCAAGGCGCTGCATCAG‑CTCCTCGGCCGAGCAGCCTTGCTGGCGCAAGGTGTCGATGATGCCGCCGGTCACCGCGTCAGCGGTGAACAGCGAACTGCTGGGGATATGGAACAGCATGCGGTCGGCA  >  NC_002947/3924469‑3924760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑