Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,932,044 C→A 7.5% R478L (CGC→CTC)  PP_3466 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,932,0440CA 7.5% 166.9 / 3.9 53R478L (CGC→CTC) PP_3466ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (28/21);  new base A (2/2);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

GCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACGGAACAACA  >  NC_002947/3931909‑3932191
                                                                                                                                       |                                                                                                                                                   
gCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGc                                                                                                                                          >  3:151496/1‑147 (MQ=255)
  cgcgATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGCGCGGCCAGCagtt                                                                                                                                      <  5:141418/149‑4 (MQ=255)
      aTCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATc                                                                                                                                  >  8:243692/1‑149 (MQ=255)
          cTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAgg                                                                                                                              <  2:84156/149‑1 (MQ=255)
           ttCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGTCGAGCGCGGCCAGCagtt                                                                                                                                      <  4:200760/140‑4 (MQ=255)
           ttCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGCGCGGCCAGCagtt                                                                                                                                      >  3:200760/1‑137 (MQ=255)
           ttCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGc                                                                                                                             >  7:231717/1‑149 (MQ=255)
               cGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAg                                                                                                                         >  7:6062/1‑149 (MQ=255)
                 cTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCg                                                                                                                       <  4:215446/149‑1 (MQ=255)
                      tGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAgg                                                                                                                  <  5:155479/149‑1 (MQ=255)
                                 aCTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAg                                                                                                       >  2:182611/1‑149 (MQ=255)
                                   tGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGc                                                                                                     >  3:137739/1‑149 (MQ=255)
                                       gAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAgg                                                                                                                              >  4:9592/1‑120 (MQ=255)
                                       gAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAgg                                                                                                                              <  3:9592/120‑1 (MQ=255)
                                       gAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTc                                                                                                 >  6:14909/1‑149 (MQ=255)
                                       gAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTc                                                                                                 <  7:243692/149‑1 (MQ=255)
                                           tGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGc                                                                                             <  8:6062/149‑1 (MQ=255)
                                                tCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCgcgc                                                                                        >  3:5857/1‑149 (MQ=255)
                                                tCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCgcgc                                                                                        >  3:188578/1‑149 (MQ=255)
                                                  gTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCgcgc                                                                                        <  7:32144/147‑1 (MQ=255)
                                                                  gCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTc                                                                      <  1:182611/149‑1 (MQ=255)
                                                                   ccGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCAACCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTc                                                                      >  7:18939/1‑148 (MQ=255)
                                                                             cAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCAc                                                           >  7:189259/1‑149 (MQ=255)
                                                                                   tGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGTCAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCCCCACCCGTTCCAGTTCATCACGGCTgg                                                     >  6:192979/1‑149 (MQ=255)
                                                                                   tGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCCCCCGTTCCAGTTCATCACGGCTgg                                                     >  7:248268/1‑149 (MQ=255)
                                                                                   tGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTgg                                                     >  6:82366/1‑149 (MQ=255)
                                                                                   tGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTgg                                                     >  1:148086/1‑149 (MQ=255)
                                                                                     cACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGc                                                                                                                     <  1:183193/83‑1 (MQ=255)
                                                                                     cACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGc                                                                                                                     >  2:183193/1‑83 (MQ=255)
                                                                                        gAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCg                                                >  6:23804/1‑149 (MQ=255)
                                                                                            aaTGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCa                                            >  8:34384/1‑149 (MQ=255)
                                                                                             aTGGCCATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCAc                                           <  5:82366/149‑1 (MQ=255)
                                                                                              tGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACg                                          <  4:151496/149‑1 (MQ=255)
                                                                                                 cGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCACAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACGAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCa                                       <  8:189259/149‑1 (MQ=255)
                                                                                                        ttgttTCTGGCCCCCGGCCAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCa                                <  5:14909/149‑1 (MQ=255)
                                                                                                        ttgttTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGc                                  >  5:57856/1‑147 (MQ=255)
                                                                                                        ttgttTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCa                                <  5:23804/149‑1 (MQ=255)
                                                                                                         tgttTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAg                               >  4:56997/1‑149 (MQ=255)
                                                                                                           tttCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAgg                              <  3:56997/148‑1 (MQ=255)
                                                                                                             tCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGtt                            <  7:34384/148‑1 (MQ=255)
                                                                                                                 gCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTgcg                       >  8:70868/1‑149 (MQ=255)
                                                                                                                 gCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTgcg                       >  3:242869/1‑149 (MQ=255)
                                                                                                                 gCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTgc                        <  4:188578/148‑1 (MQ=255)
                                                                                                                  cccccGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTgcgc                      >  4:163463/1‑149 (MQ=255)
                                                                                                                           aaCTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGcc             >  7:48433/1‑149 (MQ=255)
                                                                                                                           aaCTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGcc             >  5:78616/1‑149 (MQ=255)
                                                                                                                            cttgctgGGCGAGCGCGGCCAGCAGGCGATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCa            >  1:148675/3‑149 (MQ=255)
                                                                                                                             ctgctgGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCAc           >  2:229482/1‑149 (MQ=255)
                                                                                                                             ctgctgGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCAc           <  4:5857/149‑1 (MQ=255)
                                                                                                                              tgctgGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACg          >  2:12966/1‑149 (MQ=255)
                                                                                                                                ctgGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTTTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACgg         <  2:148086/148‑1 (MQ=255)
                                                                                                                                ctgGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACGGa        <  3:163463/149‑1 (MQ=255)
                                                                                                                                   gCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACGGaa       <  1:12966/147‑1 (MQ=255)
                                                                                                                                      gcgcTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACGGAacaaca  >  7:237907/1‑149 (MQ=255)
                                                                                                                                       |                                                                                                                                                   
GCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACGGAACAACA  >  NC_002947/3931909‑3932191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑