| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,974,681 | C→T | 10.8% | intergenic (‑209/‑132) | PP_3503 ← / → PP_3504 | sigma‑54 dependent transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,974,681 | 0 | C | T | 10.8% | 93.9 / 3.3 | 37 | intergenic (‑209/‑132) | PP_3503/PP_3504 | sigma‑54 dependent transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (16/17); new base T (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01 | |||||||||||
CAGTAGCAACGAAGTTGACGGTTCCCAGTACCGTCTGTCTGCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCGATGTTTGGGACCATAGAGAGACCGAACCA > NC_002947/3974538‑3974813 | cAGTAGCAACGAAGTTGACGGTTCCCAGTACCGTCTGTCTGCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAAtg > 8:42537/1‑149 (MQ=255) gCAACGAAGTTGACGGTTCCCAGTACCGTCTGTCTGCCGAATCATTTTTTTAAAAACCGCCAAGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAAtgtgtg > 1:204099/1‑148 (MQ=255) cAACGAAGTTGACGGTTCCCAGTACCGTCTGTCTGCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGAc > 7:85760/1‑149 (MQ=255) gTTGACGGTTCCCAGTACCGTCTGTCTGCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATGACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCa < 8:85760/149‑1 (MQ=255) tACCGTCTGTCTGCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATc < 6:134966/149‑1 (MQ=255) gtctgCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACa > 7:25166/1‑149 (MQ=255) gCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAAc > 8:52691/1‑149 (MQ=255) gAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAAtgtgt < 4:241993/109‑1 (MQ=255) gAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAAtgtgt > 3:241993/1‑109 (MQ=255) aCAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGAc < 5:146149/128‑1 (MQ=255) aCAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGAc > 6:146149/1‑128 (MQ=255) cAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTcg < 7:52691/148‑1 (MQ=255) aaaCCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTcgc > 4:258662/1‑148 (MQ=255) cACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATg < 7:42537/149‑1 (MQ=255) cGGGAATTTTCGCACTTTATCGAAATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACACACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAg < 1:73642/149‑1 (MQ=255) ggAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGc > 6:24573/1‑149 (MQ=255) gAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGc > 8:25090/1‑148 (MQ=255) tCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTTAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCACGTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACtt < 1:191584/148‑1 (MQ=255) gCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGaa < 8:25166/149‑1 (MQ=255) tATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAAc < 5:24573/148‑1 (MQ=255) tCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAAcgcg > 1:183768/1‑149 (MQ=255) tCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAAcgc < 2:204099/148‑1 (MQ=255) tcATCACATGGCGGCGCATGAAAGAACAGCGTAGACCCTGAAAAATATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGaa < 7:223337/137‑1 (MQ=255) aCATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGac > 6:263099/1‑149 (MQ=255) aCATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCAGAAAAAAATTTACAGCCAATCAGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCCCTGCACTTCGCCATCAATGAGGCGAAATTGAAgatatagg > 8:223337/1‑139 (MQ=255) acaTGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGacttca < 7:25090/148‑1 (MQ=255) ggcggcGCGGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGacttcacttc > 8:232547/1‑148 (MQ=255) aGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCGATGTTTgg < 2:183768/149‑1 (MQ=255) gCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCGATGTTTGGGa > 4:171157/1‑149 (MQ=255) aaGAACAGCGTCGACCCTGAAAAAAATTTACAGTCAATGTGTGACTTACCCACGaacaac > 8:87636/1‑60 (MQ=255) aaGAACAGCGTCGACCCTGAAAAAAATTTACAGTCAATGTGTGACTTACCCACGaacaac > 6:171770/1‑60 (MQ=255) aaGAACAGCGTCGACCCTGAAAAAAATTTACAGTCAATGTGTGACTTACCCACGaacaac < 7:87636/60‑1 (MQ=255) aaGAACAGCGTCGACCCTGAAAAAAATTTACAGTCAATGTGTGACTTACCCACGaacaac < 5:171770/60‑1 (MQ=255) aCAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCGATGTTTGGGACCATa < 5:263099/148‑1 (MQ=255) ccTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCGATGTTTGGGACCATAGAGAGACCGAAc < 3:258662/149‑1 (MQ=255) tGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGATGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACCAGGACTTCACTTCGATGTTTGGGACCATATAGAGACCGAACCa > 7:43796/1‑149 (MQ=255) aTTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCCATGTTTgg < 8:190226/120‑1 (MQ=255) | CAGTAGCAACGAAGTTGACGGTTCCCAGTACCGTCTGTCTGCCGAATCATTTTTTTACAAACCGCCACGGGAATTTTCGCACTTTATCGACATCACATGGCGGCGCAGGCAAGAACAGCGTCGACCCTGAAAAAAATTTACAGCCAATGTGTGACTTACCCACGAACAACGAGCATCA‑GTAGACATAACCCCGCGCTGCACTTCGCGATCAATGAGGCGACTTTGAAGCAACGCGGACTTCACTTCGATGTTTGGGACCATAGAGAGACCGAACCA > NC_002947/3974538‑3974813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |