Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,985,939 G→C 7.5% K72N (AAG→AAC) ‡ oplB → 5‑oxoprolinase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,985,9390GC 7.5% 163.2 / 2.6 52K72N (AAG→AAC) ‡oplB5‑oxoprolinase B
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base C (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAAAGACCGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTGGACCGTTTG  >  NC_002947/3985803‑3986085
                                                                                                                                         |                                                                                                                                                  
tgGAAAAGACGGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCTCGGCCACGC‑ACTTCATGGCCg                                                                                                                                          <  4:246594/147‑1 (MQ=255)
tgGAAAAGACCGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCTCGGCCACGC‑ACTTCATGGCCg                                                                                                                                          >  3:246594/1‑147 (MQ=255)
    aaaGACCGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGttct                                                                                                                                    >  7:142813/1‑149 (MQ=255)
       gACCGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCg                                                                                                                                 >  3:112053/1‑149 (MQ=255)
       gACCGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCg                                                                                                                                 >  5:238494/1‑149 (MQ=255)
         ccGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGc                                                                                                                               >  5:259739/1‑149 (MQ=255)
         ccGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGc                                                                                                                               <  2:97666/149‑1 (MQ=255)
                     gtgtgtTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCACGC‑ACTTCATGGCCggg                                                                                                                                        <  8:123983/128‑3 (MQ=255)
                     gtgtgtTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCAACCTGGGCTCGCTGATCCCGGCCACGC‑ACTTCATGGCCggg                                                                                                                                        >  7:123983/1‑126 (MQ=255)
                        tgtTCAACCTCGCCCACGACTATTCCAACTCACTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTCGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGATGTCGTCGCCGaa                                                                                                                <  5:222004/149‑1 (MQ=255)
                         gtTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAg                                                                                                               >  3:56243/1‑149 (MQ=255)
                          ttCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGc                                                                                                                               >  5:121753/1‑132 (MQ=255)
                          ttCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGc                                                                                                                               <  6:121753/132‑1 (MQ=255)
                          ttCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAg                                                                                                               <  1:35909/148‑1 (MQ=255)
                                   gCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGAt                                                                                                     <  6:259739/149‑1 (MQ=255)
                                         gACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGAAATCGCCGAAGGCGACGTGATCTACca                                                                                               >  2:177642/1‑149 (MQ=255)
                                         gACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGGGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACca                                                                                               <  1:242026/149‑1 (MQ=255)
                                              ttCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAAc                                                                                           >  6:45935/1‑148 (MQ=255)
                                                 cAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGAcc                                                                                       >  6:234215/1‑149 (MQ=255)
                                                 cAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGAc                                                                                        <  5:102744/148‑1 (MQ=255)
                                                              gACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAggg                                                                          <  5:45935/149‑1 (MQ=255)
                                                                ccACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGaa                                                                                                                >  2:271276/1‑109 (MQ=255)
                                                                ccACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGaa                                                                                                                <  1:271276/109‑1 (MQ=255)
                                                                  aCTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGc                                                                      <  6:238494/149‑1 (MQ=255)
                                                                   cTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGAAATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGcc                                                                     <  1:177642/149‑1 (MQ=255)
                                                                          gAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATtct                                                              >  5:108647/1‑149 (MQ=255)
                                                                           aaaTGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATtctc                                                             <  8:142813/149‑1 (MQ=255)
                                                                             aTGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGa                                                           >  4:111877/1‑149 (MQ=255)
                                                                                 tCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAAc                                                                                           <  1:71670/113‑1 (MQ=255)
                                                                                 tCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAAc                                                                                           >  2:71670/1‑113 (MQ=255)
                                                                                   cTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGActgct                                                      <  5:234215/148‑1 (MQ=255)
                                                                                    tGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGa                                                           >  1:257901/1‑142 (MQ=255)
                                                                                    tGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGa                                                           <  2:257901/142‑1 (MQ=255)
                                                                                      cAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACtgctgc                                                    <  4:112053/147‑1 (MQ=255)
                                                                                      cAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCAt                                                  <  6:108647/149‑1 (MQ=255)
                                                                                          gCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGAGAGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGActgct                                                      >  1:191619/1‑142 (MQ=255)
                                                                                          gCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGActgct                                                      <  2:191619/141‑1 (MQ=255)
                                                                                             aGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACaaa                                           >  4:172194/1‑149 (MQ=255)
                                                                                                aCATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCg                                        <  2:22039/149‑1 (MQ=255)
                                                                                                 cATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCTGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCgg                                       <  3:240386/149‑1 (MQ=255)
                                                                                                  aTTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGgt                                      <  3:172194/149‑1 (MQ=255)
                                                                                                       cATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTAt                                  >  4:31119/1‑148 (MQ=255)
                                                                                                        aTCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTAtt                                 >  6:26271/1‑148 (MQ=255)
                                                                                                        aTCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTa                                >  7:153801/1‑149 (MQ=255)
                                                                                                                gggCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGAc                         >  1:203847/1‑148 (MQ=255)
                                                                                                                 ggCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGa                       <  8:153801/149‑1 (MQ=255)
                                                                                                                       cTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGgt                 >  5:114583/1‑149 (MQ=255)
                                                                                                                              cGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTg           >  6:96791/1‑148 (MQ=255)
                                                                                                                               ggCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTGGa         <  4:56243/149‑1 (MQ=255)
                                                                                                                                   aTGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTGGACCGt     >  2:3949/1‑149 (MQ=255)
                                                                                                                                    tGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTGGACCGtt    <  5:96791/149‑1 (MQ=255)
                                                                                                                                        aGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTGGACCGTTTg  <  3:111877/148‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
TGGAAAAGACCGGGCGCTCCAGTGTGTTCAACCTCGCCCACGACTATTCCAACTCGCTGTTCGACCACTTGCCGGAAATGATCCTGCAGGGCCAGGACATTCCCATCCACCTGGGCTCGCTGATCCCGGCCATGA‑AGTGCGTGGCCGAGTTCTTCGGCGACGACATCGCCGAAGGCGACGTGATCTACCACAACGACCCCGCCTACAAGGGCAGCCATATTCTCGACTGCTGCATGTACAAACCGGTGTATTACCAGGACGAGCTGGTGTTCTGGACCGTTTG  >  NC_002947/3985803‑3986085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑