Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,996,897 G→T 8.0% P89H (CCT→CAT)  PP_3525 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,996,8970GT 8.0% 149.4 / 4.0 50P89H (CCT→CAT) PP_3525hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

ACCAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTG  >  NC_002947/3996750‑3997040
                                                                                                                                                   |                                                                                                                                               
aCCAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAgg                                                                                                                                                >  1:250223/1‑149 (MQ=255)
       cGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgcgc                                                                                                                                         <  3:61933/149‑1 (MQ=255)
                gcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                >  3:225574/1‑149 (MQ=255)
                 cAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                <  1:264497/148‑1 (MQ=255)
                            aCTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATAcc                                                                                                                    >  8:142889/1‑148 (MQ=255)
                             cTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACg                                                                                                                          >  8:27727/1‑142 (MQ=255)
                             cTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACg                                                                                                                          <  7:27727/142‑1 (MQ=255)
                             cTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATa                                                                                                                      >  5:91022/1‑146 (MQ=255)
                             cTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATa                                                                                                                      <  6:91022/146‑1 (MQ=255)
                                 aCCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAgcggc                                                                                                               <  8:15997/149‑1 (MQ=255)
                                        cctcctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTgcgc                                                                                                                                         <  7:165423/116‑1 (MQ=255)
                                        cctcctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTgcgc                                                                                                                                         >  8:165423/1‑116 (MQ=255)
                                          tcctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTc                                                                                                      >  4:236523/1‑149 (MQ=255)
                                            ctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTcc                                                                                                     <  3:236523/148‑1 (MQ=255)
                                               cccGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAg                                                                                                   <  2:258341/147‑1 (MQ=255)
                                               cccGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAg                                                                                                   >  1:258341/1‑147 (MQ=255)
                                               cccGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGt                                                                                                 <  5:257768/149‑1 (MQ=255)
                                                      tACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCtt                                                                                          <  3:200896/149‑1 (MQ=255)
                                                      tACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCtt                                                                                          <  2:250223/149‑1 (MQ=255)
                                                        ccGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTgcgc                                                                                                                                         <  5:121702/100‑1 (MQ=255)
                                                        ccGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTgcgc                                                                                                                                         >  6:121702/1‑100 (MQ=255)
                                                           gCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAg                                                                                                   <  6:147533/135‑1 (MQ=255)
                                                           gCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAg                                                                                                   >  5:147533/1‑135 (MQ=255)
                                                                  aCTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGATGCTCGGc                                                                              >  6:273963/1‑149 (MQ=255)
                                                                    tCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGc                                                                              >  3:234483/1‑147 (MQ=255)
                                                                    tCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGc                                                                              >  2:79246/1‑147 (MQ=255)
                                                                            cACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTgccgcc                                                                    >  7:16768/1‑149 (MQ=255)
                                                                               ggTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGt                                                                                                           <  8:7936/107‑1 (MQ=255)
                                                                               ggTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGt                                                                                                           >  7:7936/1‑107 (MQ=255)
                                                                                    agcagcACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAg                                                            >  3:157451/1‑149 (MQ=255)
                                                                                     gcagcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGc                                                           >  3:227329/1‑149 (MQ=255)
                                                                                       agcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCtt                                                         <  4:234483/149‑1 (MQ=255)
                                                                                        gcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTg                                                        <  1:50699/149‑1 (MQ=255)
                                                                                        gcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTg                                                        >  5:110416/1‑149 (MQ=255)
                                                                                        gcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTg                                                        >  6:12439/1‑149 (MQ=255)
                                                                                         caCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGt                                                                          <  6:67074/130‑1 (MQ=255)
                                                                                         caCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGt                                                                          >  5:67074/1‑130 (MQ=255)
                                                                                                     gTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCa                                              <  7:157606/146‑1 (MQ=255)
                                                                                                     gTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCa                                              >  8:157606/1‑146 (MQ=255)
                                                                                                            cgcATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCgcgcgc                                    <  1:79246/149‑1 (MQ=255)
                                                                                                               aTGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGccac                                 <  4:225574/149‑1 (MQ=255)
                                                                                                                   ccGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGt                             <  5:12439/149‑1 (MQ=255)
                                                                                                                         cagcgAACAGTTTGCTGATCAGCGCAGGCATGGTCTTAATGTCGGCCAAGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGAGCAGCTTGGCACCGTCGATATCGCGCGCCACCAGTAATATc                       <  6:110416/147‑1 (MQ=255)
                                                                                                                                   tttGCTGATCAGCGCAGGCATGCGCTGAAAGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCGTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCgg             >  4:46402/1‑149 (MQ=255)
                                                                                                                                      gCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATa                                                                                                                      >  2:159071/1‑41 (MQ=255)
                                                                                                                                       cTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCAc          <  5:273963/148‑1 (MQ=255)
                                                                                                                                        tGATCAGCGCAGGCATGCGCTGAATGTCGGCCACCAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGcca                                  >  1:75839/1‑123 (MQ=255)
                                                                                                                                        tGATCAGCGCAGGCATGCGCTGAATGTCGGCCACAAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGcca                                  <  2:75839/123‑1 (MQ=255)
                                                                                                                                               cgcAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGTACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTg  >  2:28897/1‑148 (MQ=255)
                                                                                                                                                  aGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCata                                            <  8:222902/103‑1 (MQ=255)
                                                                                                                                                  aGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCata                                            >  7:222902/1‑103 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACCAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTG  >  NC_002947/3996750‑3997040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑