| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,022,306 | G→C | 14.3% | intergenic (‑1/+667) | PP_3547 ← / ← emrB | NAD(P)‑binding oxidoreductase/multidrug transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,022,306 | 0 | G | C | 14.3% | 117.8 / 13.7 | 49 | intergenic (‑1/+667) | PP_3547/emrB | NAD(P)‑binding oxidoreductase/multidrug transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (23/19); new base C (3/4); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
GCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGA > NC_002947/4022159‑4022453 | gcgcTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATgg < 6:222742/149‑1 (MQ=255) cgcTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGa > 7:31278/1‑149 (MQ=255) ttGGACTCACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGt < 4:37216/149‑1 (MQ=255) ggAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTc < 8:144085/148‑1 (MQ=255) gAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTc > 7:230918/1‑147 (MQ=255) cGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCt < 8:130064/148‑1 (MQ=255) aTCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCa < 6:153798/137‑1 (MQ=255) aTCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCa > 5:153798/1‑137 (MQ=255) aGCCCACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATg > 7:178889/1‑149 (MQ=255) ccAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGc > 6:81285/1‑138 (MQ=255) ccAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGc < 5:81285/138‑1 (MQ=255) aaCCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGc < 3:252596/149‑1 (MQ=255) ccactGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGcccc < 7:164427/133‑1 (MQ=255) ccactGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGcccc > 8:164427/4‑136 (MQ=255) tctGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGccc < 2:175926/133‑1 (MQ=255) tctGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGccc > 1:175926/1‑133 (MQ=255) tctGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCCGAAGGCCCCatcctg < 1:110951/141‑6 (MQ=255) tctGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCCGAAGGCCCCatcctg > 2:110951/1‑136 (MQ=255) cttttgatctggcttttgatctggCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCgg < 8:230918/146‑1 (MQ=255) tttgatctggcttttgatctggctTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCgg > 7:42179/1‑149 (MQ=255) tgatctggcttttgatctggctttTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGt < 6:158085/148‑1 (MQ=255) ctggcttttgatctggcttttgatCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑Ag > 5:27136/1‑117 (MQ=255) ctggcttttgatctggcttttgatCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑Ag < 6:27136/117‑1 (MQ=255) gcttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCgg > 8:239609/1‑149 (MQ=40) ttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCgg > 5:222950/1‑147 (MQ=38) tgctctggcttttgctctTGATCTTGCTCTTGATCTTCGCGACTTCCGGAGGCCGAACGCCGGCCTTGCGGAGGCAGGTGACGGGCCTGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGc < 1:128666/145‑1 (MQ=11) gatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCa < 2:12988/99‑1 (MQ=255) gcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCg > 5:196010/1‑148 (MQ=32) tgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCg > 6:103845/1‑147 (MQ=32) gatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGa < 6:196010/148‑1 (MQ=32) tcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcg > 5:220368/1‑149 (MQ=32) tgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgcag > 8:104836/1‑149 (MQ=32) tcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagggcagcg > 5:76917/1‑148 (MQ=17) ttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGcgcagcgcag > 6:64413/1‑149 (MQ=32) gACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGcc < 6:222950/149‑1 (MQ=32) ggaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCTTGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGgatgat < 6:78549/149‑1 (MQ=17) aggCCGAACGCTGGCCTTGCGGAGGCTGGTGACGGGTATGTATGCGCTTCAAGCGCTG‑AGGCCCCTGGTTGGGGCGTTCGGCGCGGTCCTGCCGGGAGCAAGGCGGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATgg < 8:42179/149‑1 (MQ=11) gAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgcagcg > 2:233937/1‑127 (MQ=32) gAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgcagcg < 1:233937/127‑1 (MQ=32) gCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑CGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGc < 5:103845/149‑1 (MQ=17) cTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGttttt > 7:95445/1‑149 (MQ=32) ttGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtttttt > 5:19895/1‑149 (MQ=32) tggCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGTTGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTAc < 8:31278/148‑1 (MQ=17) cttGACGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGa < 2:47037/122‑1 (MQ=12) cttGACGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGa > 1:47037/3‑124 (MQ=12) gACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtttttt > 8:201262/1‑135 (MQ=32) gACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtttttt < 7:201262/135‑1 (MQ=32) gACGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGCGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTc > 2:140254/1‑149 (MQ=12) gCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGtt > 6:55932/1‑149 (MQ=32) aTGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGttt > 1:190224/1‑148 (MQ=32) ggATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGCGCCTTCGGCGCGGTCCTGCCGGGACCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGCGGCCGGATGATGGGAGCGCAGCCTTTTTTCGTTACTTTTTGTCGCGATTGa > 8:68933/1‑148 (MQ=11) | GCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGA > NC_002947/4022159‑4022453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |