Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,022,807 C→G 9.5% intergenic (‑502/+166) PP_3547 ← / ← emrB NAD(P)‑binding oxidoreductase/multidrug transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,022,8070CG 9.5% 122.9 / 4.9 42intergenic (‑502/+166)PP_3547/emrBNAD(P)‑binding oxidoreductase/multidrug transporter permease
Reads supporting (aligned to +/- strand):  ref base C (19/19);  new base G (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

TCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCG  >  NC_002947/4022662‑4022951
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tCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATg                                                                                                                                               >  1:65469/1‑149 (MQ=255)
  aaaCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGc                                                                                                                                              <  8:24386/148‑1 (MQ=255)
     cgcgACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGt                                                                                                                                          <  3:56411/149‑1 (MQ=255)
       cgACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCa                                                                                                                                        <  5:94325/149‑1 (MQ=255)
          cAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATGGATGCCCGTCaagc                                                                                                                                     <  7:240168/149‑4 (MQ=255)
           aaaaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATGGATGCCCGTCaagc                                                                                                                                     >  8:240168/1‑145 (MQ=255)
           aaaaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTc                                                                                                                                    <  2:65469/149‑1 (MQ=255)
           aaaaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTc                                                                                                                                    >  5:207442/1‑149 (MQ=255)
                gTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc                                                                                                                               <  3:53082/149‑1 (MQ=255)
                gTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCCCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc                                                                                                                               >  6:224518/1‑149 (MQ=255)
                     cAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATGGATGCCCGTc                                                                                                                                         >  3:42605/1‑134 (MQ=255)
                     cAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATGGATGCCCGTc                                                                                                                                         <  4:42605/134‑1 (MQ=255)
                                 gCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTc                                                                                                                <  6:207442/147‑1 (MQ=37)
                                 gCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCgg                                                                                                              <  6:47418/149‑1 (MQ=37)
                                      tATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTACCGGGAGGACCGCGCTGCCGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATGCCTGCCCGTCCCCTCCCTTCGCAACCCCAGCGGTCGgccgcc                                                                                                         >  7:21963/1‑149 (MQ=11)
                                                        cTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCgcgg                                                                                       >  8:74325/1‑149 (MQ=38)
                                                         tACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCt                                                                                                                      >  8:174945/1‑117 (MQ=35)
                                                         tACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCt                                                                                                                      <  7:174945/117‑1 (MQ=35)
                                                              gccGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTg                                                                                 <  8:230561/148‑1 (MQ=39)
                                                                cGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTgg                                                                                <  8:29513/148‑1 (MQ=40)
                                                                        ccTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgcg                                                                       >  1:256595/1‑149 (MQ=255)
                                                                         cTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgcgc                                                                      <  7:220246/149‑1 (MQ=255)
                                                                         cTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgcgc                                                                      >  3:238011/1‑149 (MQ=255)
                                                                         cTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCAGCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgcgc                                                                      >  5:91760/1‑149 (MQ=255)
                                                                              tCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCt                                                                   >  6:156080/1‑147 (MQ=255)
                                                                                    cTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCa                                                           <  2:256595/149‑1 (MQ=255)
                                                                                           ccGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCa                                                     >  2:181792/1‑148 (MQ=255)
                                                                                             gggAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCaaaa                                                  <  5:253587/149‑1 (MQ=255)
                                                                                              ggaggaCCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAg                                                 <  5:224518/149‑1 (MQ=255)
                                                                                                     cgcgCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagc                                          <  7:74325/149‑1 (MQ=255)
                                                                                                          gctgggCCCCAGGATGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagag                                     >  1:34209/5‑149 (MQ=255)
                                                                                                            cAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagc                                    >  2:227556/1‑148 (MQ=255)
                                                                                                               gCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAAt                                                       >  6:161429/1‑126 (MQ=255)
                                                                                                               gCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAAt                                                       <  5:161429/126‑1 (MQ=255)
                                                                                                                   cATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagc                              >  3:243572/1‑147 (MQ=255)
                                                                                                                              ccAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcga                      >  2:223757/1‑144 (MQ=255)
                                                                                                                              ccAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcga                      <  1:223757/144‑1 (MQ=255)
                                                                                                                                aGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcgagagcgag               <  7:36845/149‑1 (MQ=255)
                                                                                                                                       gACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcgagagcgagagcga          >  2:215575/1‑147 (MQ=255)
                                                                                                                                        aCGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAgcgagagcgagagcgagagcgagagcgagagcgaga        <  5:156080/148‑1 (MQ=255)
                                                                                                                                          gggCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCa     >  3:171429/1‑149 (MQ=255)
                                                                                                                                             cATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCg  >  2:155700/1‑149 (MQ=255)
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TCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCG  >  NC_002947/4022662‑4022951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑