Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,053,483 C→T 12.5% G19G (GGC→GGT)  PP_3573 → monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,053,4830CT12.5% 77.8 / 4.1 32G19G (GGC→GGT) PP_3573monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (13/15);  new base T (2/2);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TCCACGGCATCCCTAGTCTGGCTCCAGGCAACGGCGGTCCTCCAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCGGGGCTG  >  NC_002947/4053344‑4053627
                                                                                                                                           |                                                                                                                                                
tCCACGGCATCCCTAGTCTGGCTCCAGGCAACGGCGGTCCTCCAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCAt                                                                                                                                          <  3:7993/148‑1 (MQ=255)
  cACGGCATCCCTAGTCTGGCTCCAGGCAACGGCGGTCCTCCAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGa                                                                                                                                        <  7:43878/148‑1 (MQ=255)
           ccTAGTCTTGCTGCATGCAACGGAGGTACTCCTAACCGAGCTGACAAAAACCGCGGGAAGAAGGGCTCGGAGTTGACTCTGAACAACCTCGAAATCGACTCCCTCGTCGTCGTGGCCTGCCTGGCCGGCGTGGCCATGAGCGAactgct                                                                                                                              <  6:216940/149‑5 (MQ=255)
                  tGGCTCCAGGCAACGGCGGTCCTCCAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGACCAAg                                                                                                                       >  7:83171/1‑149 (MQ=255)
                                ggcggTCCTCCAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCa                                                                                                         >  4:99305/1‑149 (MQ=255)
                                     tcctccAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTgg                                                                                                    <  6:105503/149‑1 (MQ=255)
                                          cAAACCGACCTGACAAAAACCGCCTTAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTAc                                                                                                 <  6:147519/147‑1 (MQ=255)
                                              ccGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAgc                                                                                           <  8:210514/149‑1 (MQ=255)
                                               cGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAgcg                                                                                          <  8:83171/149‑1 (MQ=255)
                                                    tGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAAcc                                                                                     >  7:46613/1‑149 (MQ=255)
                                                     gACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGTGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCg                                                                                    >  5:25548/1‑149 (MQ=255)
                                                     gACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGCCGCCGGCCAGGCCGGTGTGGCCATGAGCGAACACCTGAGCAAGCATGGCGAGCCGCACCTGGTACTGGAGCGCAACCg                                                                                    >  3:190036/1‑149 (MQ=255)
                                                        aaaaaCCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCCGCGTGGCCATGAGCGAACACCTGAGCAAGCGTGGCGGGCCGCCCCTGGGACAGGCGCGCCACCGCAt                                                                                 >  6:243437/1‑149 (MQ=255)
                                                                           gCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTAcc                                                              >  5:108237/1‑149 (MQ=255)
                                                                                    tGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTgg                                                     <  1:185737/149‑1 (MQ=255)
                                                                                     gACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGc                                                           <  6:196693/142‑1 (MQ=255)
                                                                                     gACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGc                                                           >  5:196693/1‑142 (MQ=255)
                                                                                        acTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACt                                                 <  6:108237/149‑1 (MQ=255)
                                                                                            aacaacCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCg                                               <  8:46613/147‑1 (MQ=255)
                                                                                            aacaacCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCt                                             <  5:243437/149‑1 (MQ=255)
                                                                                                acCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTc                                         <  3:99305/149‑1 (MQ=255)
                                                                                                                  tcgtcgtcgGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTgg                       >  1:79371/1‑149 (MQ=255)
                                                                                                                     tcgtcgGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCAc                    >  6:74129/1‑149 (MQ=255)
                                                                                                                      tggccacgcCGGCCTGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACg                   >  2:202809/7‑149 (MQ=255)
                                                                                                                           gcgcCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGc              >  7:204904/1‑149 (MQ=255)
                                                                                                                              ccGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTc           >  3:19018/1‑149 (MQ=255)
                                                                                                                              ccGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTc           <  5:74129/149‑1 (MQ=255)
                                                                                                                                   cAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCggg      >  8:26334/1‑149 (MQ=255)
                                                                                                                                   cAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCggg      >  1:10812/1‑149 (MQ=255)
                                                                                                                                    aGGCCGGTGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCAGCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCGGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCgggg     <  4:190036/149‑1 (MQ=255)
                                                                                                                                    aGGCCGGTGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCgggg     <  6:25548/149‑1 (MQ=255)
                                                                                                                                       ccGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCGGGGCTg  <  8:204904/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
TCCACGGCATCCCTAGTCTGGCTCCAGGCAACGGCGGTCCTCCAAACCGACCTGACAAAAACCGCCTGAACAAGGGCTCGGACATGACACTGAACAACCTCGAAATCGACACCCTCGTCGTCGGCGCCGGCCAGGCCGGCGTGGCCATGAGCGAACACCTGAGCAAGCTTGGCGTGCCGCACCTGGTACTGGAGCGCAACCGCATCGCGCAGGCCTGGCGTACCGGCCGCTGGGACTCGCTGGTCGCCAACGGCCCGGCCTGGCACGACCGCTTCCCGGGGCTG  >  NC_002947/4053344‑4053627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑