Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,091,457	G→T	6.3%	V22V (GTG→GTT)	garD →	galactarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,091,457	0	G	T	6.3%	200.5 / 3.8	63	V22V (GTG→GTT)	garD	galactarate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base T (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-01

GAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCAAAT  >  NC_002947/4091313‑4091597                                                                                                                                                 |                                                                                                                                             gAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTTATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGgtcg                                                                                                                                          >  3:119556/1‑149 (MQ=255)     aaaCACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGgtcgtcgt                                                                                                                                      <  1:122337/149‑1 (MQ=255)      aaCACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACCACGACGTTgtcgtcgtc                                                                                                                                     <  3:77560/149‑1 (MQ=255)        cacGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCaa                                                                                                                                   <  3:233823/149‑1 (MQ=255)         acGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGgtcgtcgtc                                                                                                                                     <  8:170196/146‑1 (MQ=255)         acGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGgtcgtcgtc                                                                                                                                     >  7:170196/1‑146 (MQ=255)            cccGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAacgac                                                                                                                               <  3:59160/149‑1 (MQ=255)            cccGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGGCGTGGTCGTCATCAacgac                                                                                                                               >  5:145601/1‑149 (MQ=255)             ccGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCCCGACGACGACAACGTCGTGGTCGTCGTCAacgacg                                                                                                                              >  4:141756/1‑149 (MQ=255)              cgcgCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGACCCGGACAAGTAGGGCCCAAACATGCAGTGGATCGAACCTGCCGACTCGCCCCGCTCCGGCCGCCTGCCCGACGACGACCACGTCGTGGTCGTCGGCAacgacgc                                                                                                                             >  3:194137/1‑148 (MQ=255)              cgcgCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTTATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGAcgg                                                                                                                             <  4:119556/149‑1 (MQ=255)                    gggTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCCACGACGGCGGCtt                                                                                                                       >  8:162795/1‑149 (MQ=255)                       tCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATACCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCCTGGTCGTCGTCAACGACGCCGGCTTggg                                                                                                                    >  8:121849/1‑149 (MQ=255)                          cccGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCg                                                                                                                  >  7:191459/1‑148 (MQ=255)                          cccGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCg                                                                                                                  >  5:209585/1‑148 (MQ=255)                                             cAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGAc                                                                                              >  3:218680/1‑149 (MQ=255)                                              aaaaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACCACGACGTTgtcgtc                                                                                                                                        <  5:201358/106‑1 (MQ=255)                                              aaaaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACCACGACGTTgtcgtc                                                                                                                                        >  6:201358/1‑106 (MQ=255)                                              aaaaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGc                                                                                                  <  5:50543/144‑1 (MQ=255)                                              aaaaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGc                                                                                                  >  6:50543/1‑144 (MQ=255)                                               aaaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACgg                                                                                            >  3:228417/1‑149 (MQ=255)                                                aaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGc                                                                                           <  6:209585/149‑1 (MQ=255)                                                              gACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCg                                                                              >  5:277138/1‑148 (MQ=255)                                                               aCAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCg                                                                              <  4:115181/147‑1 (MQ=255)                                                                cAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAg                                                                           <  4:228417/149‑1 (MQ=255)                                                                 aaGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAgg                                                                          >  3:95085/1‑149 (MQ=255)                                                                 aaGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAgg                                                                          >  5:170943/1‑149 (MQ=255)                                                                  aGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGc                                                                         >  4:6461/1‑149 (MQ=255)                                                                    tAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAg                                                                           >  8:222493/1‑145 (MQ=255)                                                                    tAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAg                                                                           <  7:222493/145‑1 (MQ=255)                                                                    tAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGt                                                                       >  4:195931/1‑149 (MQ=255)                                                                    tAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGt                                                                       <  3:34018/149‑1 (MQ=255)                                                                       ggCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGcc                                                                    <  6:277138/149‑1 (MQ=255)                                                                            aaaCATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCaaa                                                               <  1:107097/149‑1 (MQ=255)                                                                                    aGTTGATCGAACATTCTGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAg                                                       >  1:156812/1‑149 (MQ=255)                                                                                      ttGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGt                                                     >  4:58572/1‑149 (MQ=255)                                                                                          tCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGc                                                  >  4:222864/1‑148 (MQ=255)                                                                                             aaCATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCAcc                                              <  6:170943/149‑1 (MQ=255)                                                                                              aCATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCg                                             >  8:57887/1‑149 (MQ=255)                                                                                                aTTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCt                                                                                         >  2:197104/1‑103 (MQ=255)                                                                                                aTTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCt                                                                                         <  1:197104/103‑1 (MQ=255)                                                                                                aTTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCgtg                                           <  7:57887/149‑1 (MQ=255)                                                                                                 ttCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCgtgt                                          <  3:58572/149‑1 (MQ=255)                                                                                                     gACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAg                                                       >  5:267729/1‑132 (MQ=255)                                                                                                     gACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAg                                                       <  6:267729/132‑1 (MQ=255)                                                                                                          gCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGcc                                  >  7:77376/1‑148 (MQ=255)                                                                                                           ccccGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCaa                                <  3:6461/149‑1 (MQ=255)                                                                                                             ccGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAgg                              <  3:222864/149‑1 (MQ=255)                                                                                                               gCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGc                            <  4:218680/149‑1 (MQ=255)                                                                                                               gCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGc                            <  3:195931/149‑1 (MQ=255)                                                                                                                   cGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAg                         <  4:95085/148‑1 (MQ=255)                                                                                                                         ccTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTgc                  >  4:189074/1‑149 (MQ=255)                                                                                                                         ccTGCACGACGACGACAACGGCGTGGTCGTCGTCAACGACGACGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTgc                  <  4:194137/149‑1 (MQ=255)                                                                                                                            gCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCAt                                      >  6:122163/1‑126 (MQ=255)                                                                                                                            gCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCAt                                      <  5:122163/126‑1 (MQ=255)                                                                                                                             cACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTg                                                    <  4:246178/111‑1 (MQ=255)                                                                                                                             cACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTg                                                    >  3:246178/1‑111 (MQ=255)                                                                                                                                    acgacAACGTCGTGGTCGTCATCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACggg       <  6:145601/149‑1 (MQ=255)                                                                                                                                      gacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGc      >  7:101909/1‑148 (MQ=255)                                                                                                                                      gacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGc      >  1:207513/1‑148 (MQ=255)                                                                                                                                          ccgtcgtTGACGACGACCACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCAAat  >  7:182184/2‑148 (MQ=255)                                                                                                                                             tcgtGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCaaa   <  5:110718/144‑1 (MQ=255)                                                                                                                                             tcgtGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCaaa   >  6:110718/1‑144 (MQ=255)                                                                                                                                                 |                                                                                                                                             GAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCAAAT  >  NC_002947/4091313‑4091597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑