Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,129,546 G→T 7.8% A55S (GCC→TCC)  PP_3633 → ArgC‑like N‑acetyl‑gamma‑glutamyl‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,129,5460GT 7.8% 162.1 / 2.8 51A55S (GCC→TCC) PP_3633ArgC‑like N‑acetyl‑gamma‑glutamyl‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (27/20);  new base T (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TTTTCATCGACGGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGC  >  NC_002947/4129400‑4129691
                                                                                                                                                   |                                                                                                                                                 
ttataaTCGACGGAGACCAAGGCACCACAGGCCTGCACATCCATCCCCGCCTGCAGGGCCCGAGCCATCTTCGCCTGCTCACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACCCCGC‑TGCGAACCGCTAAACAGTGCCGACATCGcc                                                                                                                                                 >  4:76478/6‑149 (MQ=255)
       cGACGGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCCATCAACAGTGCCGACATCGCCCTGCtgt                                                                                                                                          >  6:151534/1‑149 (MQ=255)
        gACGGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCtgt                                                                                                                                          <  1:39774/148‑1 (MQ=255)
         aCGGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGtgc                                                                                                                                        <  8:52834/149‑1 (MQ=255)
           ggCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGtgcct                                                                                                                                      >  4:187155/1‑149 (MQ=255)
                          acaGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGAcgccgc                                                                                                                       <  3:50802/149‑1 (MQ=255)
                                 tGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAg                                                                                                                >  7:125505/1‑149 (MQ=255)
                                   cAGATCCATGCCCGCATGCAGGGCCGGAGCGATCTTCGCCTGCTGACGCTCCCCGAGGCCGAGCGCAAGGACGCGCAACGCCGC‑GGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGc                                                                                                              <  6:254933/149‑1 (MQ=255)
                                           tGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGTCCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCg                                                                                                      >  6:42509/1‑149 (MQ=255)
                                            gCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGTTCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGa                                                                                                                 <  8:106892/137‑1 (MQ=255)
                                            gCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGCTCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGa                                                                                                                 >  7:106892/1‑137 (MQ=255)
                                                       gggCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAAcc                                                                                          <  2:28440/149‑1 (MQ=255)
                                                            ggAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCTCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAg                                                                                     >  8:238426/1‑149 (MQ=255)
                                                            ggAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCTCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAg                                                                                     >  8:235004/1‑149 (MQ=255)
                                                             gAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGAcgccg                                                                                                                        <  6:170132/113‑1 (MQ=255)
                                                                  aTCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGACCGTGGCCGCAATCCCCAACCCCCAGGTGCgg                                                                               >  1:107064/1‑149 (MQ=255)
                                                                       cGCCTGCTGTCCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGt                                                                             <  5:42509/146‑1 (MQ=255)
                                                                           tgctgACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGt                                                                                                           <  7:91829/112‑1 (MQ=255)
                                                                           tgctgACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGt                                                                                                           >  8:91829/1‑112 (MQ=255)
                                                                                 cccTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGc                                                                <  8:125505/149‑1 (MQ=255)
                                                                                  ccTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGa                                                                       <  3:91899/141‑1 (MQ=255)
                                                                                  ccTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGa                                                                       >  4:91899/1‑141 (MQ=255)
                                                                                   cTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCGTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCt                                                               >  1:44407/1‑149 (MQ=255)
                                                                                   cTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTc                                                              >  5:137457/1‑149 (MQ=255)
                                                                                      cccGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCgg                                                            <  2:126958/148‑1 (MQ=255)
                                                                                      cccGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCgg                                                            <  2:44407/148‑1 (MQ=255)
                                                                                       ccGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGgcg                                                          >  1:48570/1‑149 (MQ=255)
                                                                                            gCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGcgcaccg                                                     >  5:52412/1‑149 (MQ=255)
                                                                                                 gcgcAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGcacca                                                >  8:89757/1‑149 (MQ=255)
                                                                                                   gcAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGTTGCGGGTGATCGACGCCAGCTCGGCGCACCGcaccaca                                              <  2:48570/149‑1 (MQ=255)
                                                                                                       ggACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAg                                          <  6:137457/149‑1 (MQ=255)
                                                                                                       ggACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAg                                          >  6:56194/1‑149 (MQ=255)
                                                                                                          cccGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCt                                       >  6:75486/1‑149 (MQ=255)
                                                                                                           ccGCAACGCCGC‑TGCGAAGCGATCACCAGTGCCGACATCGCCCTGCTGTGCCTGCCCGCCGACGCCGCCCGCGAGGCCGTGGCCGCATTCCCCAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACAACAGGCTg                                      >  5:210856/1‑149 (MQ=255)
                                                                                                             gCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACaa                                                                                            >  2:101367/1‑93 (MQ=255)
                                                                                                             gCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACaa                                                                                            <  1:101367/93‑1 (MQ=255)
                                                                                                                aCGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGTACCGcaccaca                                              <  5:177720/136‑1 (MQ=255)
                                                                                                                aCGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGACAGCTCGGCGAACCGcaccaca                                              >  6:177720/1‑136 (MQ=255)
                                                                                                                     gc‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGc                            >  6:221667/1‑149 (MQ=255)
                                                                                                                     gc‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGc                            <  5:221667/149‑1 (MQ=255)
                                                                                                                         gCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGgt                                   <  3:51316/139‑1 (MQ=255)
                                                                                                                         gCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGgt                                   >  4:51316/1‑139 (MQ=255)
                                                                                                                          cGAAGCGATCAACAGTGCCGACATCTCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGc                        <  7:235004/149‑1 (MQ=255)
                                                                                                                          cGAAGCGATCAACAGTGCCGACATCTCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGc                        <  7:238426/149‑1 (MQ=255)
                                                                                                                            aaGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCg                      >  5:65145/1‑149 (MQ=255)
                                                                                                                               cGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAg                   >  6:213611/1‑149 (MQ=255)
                                                                                                                                  tCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCtt                >  7:188181/1‑149 (MQ=255)
                                                                                                                                    aaCAGTGCCAACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCgg                                                            >  2:188695/1‑103 (MQ=255)
                                                                                                                                    aaCAGTGCCAACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCgg                                                            <  1:188695/103‑1 (MQ=255)
                                                                                                                                             gACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGc      >  6:109088/1‑148 (MQ=255)
                                                                                                                                                aTCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGc  >  5:276402/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTTTCATCGACGGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGC‑TGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGC  >  NC_002947/4129400‑4129691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑