| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,160,661 | G→C | 10.8% | intergenic (+27/+94) | PP_3662 → / ← PP_3663 | hypothetical protein/GGDEF domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,160,661 | 0 | G | C | 10.8% | 103.2 / 4.7 | 37 | intergenic (+27/+94) | PP_3662/PP_3663 | hypothetical protein/GGDEF domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (16/17); new base C (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01 | |||||||||||
CACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCCC > NC_002947/4160515‑4160809 | cACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGa < 6:31129/149‑1 (MQ=255) ggTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGc < 2:217462/149‑1 (MQ=255) aCAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCg < 2:42976/148‑1 (MQ=255) cAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTc < 1:142050/149‑1 (MQ=255) cTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCgggg < 6:207974/149‑1 (MQ=255) gACCCGCTGGGCCGGCTACTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCggggg < 7:154226/147‑1 (MQ=255) gCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCCGCGCGGCGTATCTACCGTGCTATCAGGTGGCATCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTg > 5:62591/1‑149 (MQ=255) gctgctGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCggg > 3:262933/1‑131 (MQ=255) gctgctGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCggg < 4:262933/131‑1 (MQ=255) tgcAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTg > 8:64418/1‑148 (MQ=255) ttCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCa > 8:68962/1‑149 (MQ=255) ttCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGt > 8:218062/1‑108 (MQ=255) ttCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGt < 7:218062/108‑1 (MQ=255) gtcgACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCAccc < 8:101914/149‑1 (MQ=255) gAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGa > 6:47369/1‑147 (MQ=255) aaGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGaaa < 7:68962/148‑1 (MQ=255) ccGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTcct > 8:252800/1‑81 (MQ=255) ccGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTcct < 7:252800/83‑3 (MQ=255) cggcggCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCca < 3:177409/124‑1 (MQ=255) cggcggCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCca > 4:177409/1‑124 (MQ=255) gcgcggcgTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAg < 7:64418/149‑1 (MQ=255) cgTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGCGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACt > 1:84734/1‑149 (MQ=255) tGTACGGTGCGATGAGGGCGCCACCTGACCCGCTGCTTTACTGCGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACGCACATGGGTTACATGAAAGGTCCCCCACCTAGGTGACTCGCt < 6:62591/149‑1 (MQ=255) aCCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCAc > 4:52617/1‑149 (MQ=255) aCCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCAc > 4:35362/1‑149 (MQ=255) tATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCg > 6:251979/1‑147 (MQ=255) aGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGtt < 3:35362/148‑1 (MQ=255) tGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGc > 4:179020/1‑138 (MQ=255) tGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGc < 3:179020/138‑1 (MQ=255) aaCCTGACCCGCTGCTTTACTGAGGAC‑GGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCaa > 6:264576/1‑143 (MQ=255) aaCCTGACCCGCTGCTTTACTGAGGAC‑GGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCaa < 5:264576/143‑1 (MQ=255) ccGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGAc < 4:257222/115‑1 (MQ=255) ccGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGAc > 3:257222/1‑115 (MQ=255) ccGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTc > 5:128291/1‑149 (MQ=255) aCTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCGGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCCACTAGGTGACTCGCTCACTGCCGGAGGTCAACAACCGCCCGCTCGAAAAACCg > 2:91472/1‑148 (MQ=255) gCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCAccgc < 6:227496/122‑1 (MQ=255) gCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCAccgc > 5:227496/1‑122 (MQ=255) ggATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGcccc > 2:249715/1‑149 (MQ=255) | CACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCCC > NC_002947/4160515‑4160809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |