Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,191,401 C→T 11.4% intergenic (‑733/‑424) PP_5612 ← / → PP_5613 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,191,4010CT11.4% 89.4 / 3.1 35intergenic (‑733/‑424)PP_5612/PP_5613hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (13/18);  new base T (2/2);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CGCGCCCTTACGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGTCCTCTGTCAAACCTACGAACGCCGAAA  >  NC_002947/4191258‑4191538
                                                                                                                                                |                                                                                                                                         
cgcgcCCTTACGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCACGCCGAt                                                                                                                                       >  4:236680/1‑148 (MQ=255)
     ccTTACGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAATCAACCTGATcggctt                                                                                                                                 >  7:192562/1‑144 (MQ=255)
       ttACGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAg                                                                                                                                    >  8:214224/1‑144 (MQ=255)
       ttACGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAg                                                                                                                                    <  7:214224/144‑1 (MQ=255)
          cGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGAtt                                                                                                                             <  6:57693/148‑1 (MQ=255)
            gggCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATtcat                                                                                                                          <  1:74840/149‑1 (MQ=255)
              gcggcTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAATCAACCTGATCGGCTTGCTtcatca                                                                                                                        <  8:192562/149‑1 (MQ=38)
              gcggcTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATtcatca                                                                                                                        <  4:15904/149‑1 (MQ=255)
                     tCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCTAATCGCATGCCCGCTCCGCTTGCGTCGAAGGTTGGTAGTTCGCCGGAGACGACAGAGCGTGATGAACCAGGCCGATCAGGTTGATTCATCACACAag                                                                                                                  >  1:205454/1‑149 (MQ=255)
                              gAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATcc                                                                                                        >  6:47343/1‑149 (MQ=255)
                                  ccGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGccc                                                                                                    >  1:177806/1‑149 (MQ=255)
                                          ggCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGTCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTgg                                                                                            >  1:173682/1‑149 (MQ=255)
                                          ggCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATtcatca                                                                                                                        >  7:26560/1‑121 (MQ=255)
                                          ggCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATacatca                                                                                                                        <  8:26560/121‑1 (MQ=255)
                                                    ggCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCt                                                                                    <  6:170056/147‑1 (MQ=255)
                                                       ccTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCAc                                                                               >  8:88116/1‑149 (MQ=255)
                                                            gggCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGc                                                                           <  2:205454/148‑1 (MQ=255)
                                                                     tCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGgc                                                                 >  3:101125/1‑149 (MQ=255)
                                                                           tcGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGa                                                            >  1:114138/1‑149 (MQ=255)
                                                                            cGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGAt                                                           <  2:114138/149‑1 (MQ=255)
                                                                              ccTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGTCGATCAGGTTGATTCATCACACAaggagg                                                                                                              <  5:26140/96‑1 (MQ=255)
                                                                              ccTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGTCGATCAGGTTGATTCATCACACAaggagg                                                                                                              >  6:26140/1‑96 (MQ=255)
                                                                               cTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGAcc                                                                                     <  5:247982/120‑1 (MQ=255)
                                                                               cTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGAcc                                                                                     >  6:247982/1‑120 (MQ=255)
                                                                                    cgcTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCa                                                   <  5:47343/149‑1 (MQ=255)
                                                                                       tCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCt                                                >  2:104450/1‑149 (MQ=255)
                                                                                         cGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCtt                                               <  2:13925/148‑1 (MQ=255)
                                                                                              gCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCa                                          <  2:177806/148‑1 (MQ=255)
                                                                                                          ggTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGTCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCaaa                               <  2:173682/147‑1 (MQ=255)
                                                                                                           gTAGTTCGCCGGAGACGACAGAGCCTGAGGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGt                             <  5:187879/148‑1 (MQ=255)
                                                                                                               ttCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGTCctct                        <  4:101125/149‑1 (MQ=255)
                                                                                                               gtCGCCGGCGACGACAGAGCCTGACGCAGCAAGCCGATCAGGCTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGTCctct                        <  4:96877/148‑1 (MQ=255)
                                                                                                                  gCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGTCCTCTGt                      <  1:104450/148‑1 (MQ=255)
                                                                                                                        gacgacAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGTCCTCTGTCAAAcc                >  4:186889/1‑148 (MQ=255)
                                                                                                                                      atgtAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGGCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACGAGGACGCCCAAAGTCCTCTGTCAAACCTACGAACGCCGaaa  <  5:139686/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                         
CGCGCCCTTACGGGGCGGCTGTCGTAAACCGAGCCCGCCTGGGGCAGGTCTCGGCCCTTCGGGCGACCATCCT‑CTCGCCTGCGCGCTCCGCTTGCGTCGAAGCTTGGTAGTTCGCCGGAGACGACAGAGCCTGATGAAGCAAGCCGATCAGGTTGATTCATCACACAAGGAGGGCATCCGCCCCCCCTTGGGTCGACCTGCCACTTGCGTCCCCTGGCGCTGATGCTCTTCATCTTTACCAGCACGCCCAAAGTCCTCTGTCAAACCTACGAACGCCGAAA  >  NC_002947/4191258‑4191538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑