Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,330,602 T→A 9.7% D16E (GAT→GAA)  PP_3802 → cation ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,330,6020TA 9.7% 111.5 / 4.0 41D16E (GAT→GAA) PP_3802cation ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (17/20);  new base A (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

ACGCATATCTCCTACGGCGATTACACCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGC  >  NC_002947/4330457‑4330741
                                                                                                                                                 |                                                                                                                                           
aCGCATATCTCCTACGGCGATTACACCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGAttt                                                                                                                                           >  8:140022/1‑148 (MQ=255)
    atatCTCCTACGGCGATTACACCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTg                                                                                                                                      >  7:88078/1‑149 (MQ=255)
        ctcCTACGGCGATTACACCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCAt                                                                                                                                  >  6:54199/1‑149 (MQ=255)
         tcCTACGGCGATTACACCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATc                                                                                                                                 >  8:90634/1‑149 (MQ=255)
          ccTACGGCGATTACACCAAGGAAAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATc                                                                                                                                 >  2:253808/1‑148 (MQ=255)
                       cacCAAGTACAAGTAGTATGTGGAAATGAAGCGCAACCTCGACACTGTGGTCCGCGCAATTCAGGAGAACCGCGCATGACCTCTGCCGGCAAGCTGGCGACAGCCTGCGGGCCGCGCATCTATTTCACTGGCATCGACCTGACCTTtgg                                                                                                                   <  7:140022/149‑1 (MQ=255)
                        acCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTgggg                                                                                                                  <  6:953/149‑1 (MQ=255)
                           aaGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGt                                                                                                               <  1:253808/149‑1 (MQ=255)
                                cAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCGTTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTAcgcg                                                                                                          <  3:137017/149‑1 (MQ=255)
                                          gTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGaa                                                                                                <  8:88078/149‑1 (MQ=255)
                                           tGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGaa                                                                                                >  5:210257/1‑148 (MQ=255)
                                               aaTGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGt                                                                                           >  1:1448/1‑149 (MQ=255)
                                                 tGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCa                                                                                         <  2:150987/149‑1 (MQ=255)
                                                       gcAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCa                                                                                   <  6:210257/149‑1 (MQ=255)
                                                            cTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTg                                                                              >  7:200220/1‑149 (MQ=255)
                                                            cTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTg                                                                              <  2:95757/149‑1 (MQ=255)
                                                                    ggtggtCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGAATTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAgcagg                                                                      >  7:134625/1‑149 (MQ=255)
                                                                    ggtggtCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGAATTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAgcagg                                                                      >  7:130941/1‑149 (MQ=255)
                                                                         tCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCg                                                                 >  6:107762/1‑149 (MQ=255)
                                                                           cgcgCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTg                                                               >  6:111756/1‑149 (MQ=255)
                                                                                  ttCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCa                                                        <  2:1448/149‑1 (MQ=255)
                                                                                     aGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCAtc                                                      <  5:111756/148‑1 (MQ=255)
                                                                                          aaCCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGAATTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGc                                                <  8:134625/149‑1 (MQ=255)
                                                                                          aaCCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGAATTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGc                                                <  8:130941/149‑1 (MQ=255)
                                                                                              gcgcATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGcccc                                             >  3:54487/1‑148 (MQ=255)
                                                                                                 cATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcg                                         >  3:30602/1‑149 (MQ=255)
                                                                                                  aTGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcgg                                        >  3:78341/1‑149 (MQ=255)
                                                                                                      ccGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcggcggc                                    <  4:54487/149‑1 (MQ=255)
                                                                                                      ccGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcggcggc                                    <  2:267356/149‑1 (MQ=255)
                                                                                                      ccGCTGCCGCAAAGCTGGCGAGAGGCTGCGGGCCGCGCATCGATTTGACTGGCATCGACCGGACGTTGGGGCGTACGCGCATCCTCGATCAGGTGAGTTTCAGAGTGGCAGCATGGCGCGTGCACGCGTTCGGCGGCGCCAAaggctgg                                    >  4:183521/1‑146 (MQ=255)
                                                                                                        gCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcggcggcgg                                  >  1:199580/1‑149 (MQ=255)
                                                                                                            ccgccAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAag                              <  8:200220/149‑1 (MQ=255)
                                                                                                              gccAAGCTGGCGACAGCCTGCGGGCTGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcggcg                                      <  7:163860/139‑1 (MQ=255)
                                                                                                              gccAAGCTGGCGACAGCCTGCGGGCTGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcggcg                                      >  8:163860/1‑139 (MQ=255)
                                                                                                                        cGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATc                  <  7:90634/149‑1 (MQ=255)
                                                                                                                        cGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATc                  <  4:78341/149‑1 (MQ=255)
                                                                                                                              ccTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGAcc            <  2:199580/149‑1 (MQ=255)
                                                                                                                                 gCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCaa                               >  7:166069/1‑127 (MQ=255)
                                                                                                                                 gCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCaa                               <  8:166069/127‑1 (MQ=255)
                                                                                                                                        gcgcATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGGCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGc  <  5:54199/149‑1 (MQ=255)
                                                                                                                                               gATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAc                                          >  2:27690/1‑102 (MQ=255)
                                                                                                                                               gATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAc                                          <  1:27690/102‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
ACGCATATCTCCTACGGCGATTACACCAAGGACAAGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGC  >  NC_002947/4330457‑4330741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑