Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,467,805 G→A 9.3% W17* (TGG→TAG) ‡ PP_3959 → voltage‑gated chloride channel family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,467,8050GA 9.3% 117.1 / 2.7 43W17* (TGG→TAG) ‡PP_3959voltage‑gated chloride channel family protein
Reads supporting (aligned to +/- strand):  ref base G (21/18);  new base A (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

GGATCGCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCTC  >  NC_002947/4467658‑4467947
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ggATCGCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTgg                                                                                                                                               <  6:200155/149‑1 (MQ=255)
  aTCGCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCGAAGTCAGCCATGTGTGGACAATGTCCGGCCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGc                                                                                                                                              >  7:112423/1‑148 (MQ=255)
        aCTCCCGGTGCGGCTACAACCTGTGTCGTGGAGGCCTGGGCGGGAAAACAAGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATg                                                                                                                                       <  4:180028/149‑1 (MQ=255)
            ccGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                   <  5:141626/149‑2 (MQ=255)
               gTGCGGCTACAACCTGTTTCGGGTAGGCCTGTGCTGGAAAACACGCCATACTCACGGATTTCCAAGTCAGCCATGTGTTGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAg                                                                                                                                <  3:166839/149‑1 (MQ=255)
                tGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGc                                                                                                                               <  8:81503/149‑1 (MQ=255)
                      tACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGc                                                                                                                                              <  1:111151/128‑1 (MQ=255)
                      tACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGc                                                                                                                                              >  2:111151/1‑128 (MQ=255)
                        cAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCg                                                                                                                       >  4:197957/1‑149 (MQ=255)
                         aaCCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGt                                                                                                                      >  4:226777/1‑149 (MQ=255)
                              gtgtCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTg                                                                                                                  >  7:25739/1‑148 (MQ=255)
                                   gggTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCgg                                                                                                            <  3:197957/149‑1 (MQ=255)
                                     gTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCgggg                                                                                                          <  1:1550/149‑1 (MQ=255)
                                                   gAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTgggg                                                                                            >  2:70746/1‑149 (MQ=255)
                                                       acacGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCg                                                                                        >  3:220730/1‑149 (MQ=255)
                                                         acGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATAGGGGCATTGCTGGTGGGGCTCGtt                                                                                      >  5:255582/1‑149 (MQ=255)
                                                           gCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTgc                                                                                    >  6:212518/1‑149 (MQ=255)
                                                              aTACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGTCGTCAGCGACTGGCGTTCTGGATCGTGGCATTGCTGGTGGGGCTCGTTGCGCt                                                                                 <  8:25739/149‑1 (MQ=255)
                                                                  tCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCt                                                                             >  4:125157/1‑149 (MQ=255)
                                                                        cTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGcc                                                                       <  1:70746/149‑1 (MQ=255)
                                                                             cAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                   >  3:208262/1‑83 (MQ=37)
                                                                             cAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                   <  4:208262/84‑2 (MQ=37)
                                                                              aaGTCAGCCATGTGTGGAGAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTGCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCt                                                                      <  6:57226/144‑1 (MQ=255)
                                                                              aaGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCACGTTGCGCTGGCTTTCGCCTgagaa                                                                 >  5:57226/1‑145 (MQ=255)
                                                                                            tgGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGtt                                                   >  1:95140/1‑149 (MQ=255)
                                                                                              gACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCAGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCTCCTGGCTAGCCGACGCGGCGTTCg                                                 >  8:128524/1‑149 (MQ=255)
                                                                                                 aaTGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCtt                                              >  1:163158/1‑149 (MQ=255)
                                                                                                 aaTGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCtt                                              >  7:38924/1‑149 (MQ=255)
                                                                                                        gACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                       <  4:185921/149‑1 (MQ=255)
                                                                                                        gACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                       <  3:226777/149‑1 (MQ=255)
                                                                                                           ccTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                       <  6:96129/146‑1 (MQ=255)
                                                                                                           ccTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                       >  5:96129/1‑146 (MQ=255)
                                                                                                           ccTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcg                                    >  6:1017/1‑149 (MQ=255)
                                                                                                                  gACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTg                             <  2:95140/149‑1 (MQ=255)
                                                                                                                      tGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAAt                         >  6:169351/1‑149 (MQ=255)
                                                                                                                           cTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcg                                    <  7:219604/133‑1 (MQ=255)
                                                                                                                           cTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcg                                    >  8:219604/1‑133 (MQ=255)
                                                                                                                              cACTCCCCCGCCGTTCACGCTGGCTAGAATGGCGTCATCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCAc                   <  6:255582/147‑1 (MQ=255)
                                                                                                                                 tCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTgg              >  6:256434/1‑149 (MQ=255)
                                                                                                                                  ctgacgccaTTCTCGCCAGCGTGAACGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGt             >  7:221877/5‑149 (MQ=255)
                                                                                                                                           ttCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtcttt    >  2:241432/1‑148 (MQ=255)
                                                                                                                                           ttCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtcttc    <  7:128524/149‑1 (MQ=255)
                                                                                                                                             cACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtctttt   <  1:241432/148‑3 (MQ=255)
                                                                                                                                              aCGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTtctc  <  8:38924/148‑1 (MQ=255)
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GGATCGCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCTC  >  NC_002947/4467658‑4467947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑